Direct Sequencing
Comparison of PCR Restriction Fragment Length Polymorphism Analysis and PCR Direct Sequencing Methods for Differentiating Helicobacter pylori ureB Gene Variants
... Most of the strains isolated from the patients with duodenal ulcer or gastric ulcer were type A, with ratios of 10/12 (83.3%) and 6/6 (100%), respectively. In contrast, 15 of 23 (65.2%) strains isolated from patients ...
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A comparison of Direct sequencing, Pyrosequencing, High resolution melting analysis, TheraScreen DxS, and the K-ras StripAssay for detecting KRAS mutations in non small cell lung carcinomas
... that direct sequencing is only of limited utility when trying to detect mutations in the KRAS gene in cancer tissues, since this method only detected KRAS mutations in 6 of the 131 DNA samples tested, even ...
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Molecular diagnosis of bacterial endocarditis by broad range PCR amplification and direct sequencing
... by direct sequencing is a relatively easy and reliable method that gives accurate results when applied to surgically removed heart valves of patients with IE and may be used for such patients as an adjunct ...
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Identification of Clinically Relevant Enterococcus Species by Direct Sequencing of groES and Spacer Region
... We determined the groESL sequences (groES, groEL, and the intergenic spacer) of 10 clinically relevant Enterococcus species and evaluated the feasibility of identifying Enterococcus species on the basis of these ...
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Direct sequencing for rapid detection of multidrug resistant <em>Mycobacterium tuberculosis</em> strains in Morocco
... Direct sequencing of amplicons was performed using a Big Dye Terminator kit version 3.1 (Applied Biosystems, Foster City, CA, USA) that includes dideoxynucleotides labeled with four fluorochromes of ...
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Direct Sequencing and Characterization of a Clinical Isolate of Epstein-Barr Virus from Nasopharyngeal Carcinoma Tissue by Using Next-Generation Sequencing Technology
... Direct sequencing of the clinical EBV isolate in the tumor reflected the natural frequencies of the EBV repertoire and thus enabled us to test the hypothesis of monoclonal expansion of EBV in NPC tumors in ...
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Direct Sequencing of Legionella pneumophila from Respiratory Samples for Sequence Based Typing Analysis
... of sequencing of Legionella pneumophila genes directly from respiratory samples and have compared the results with those derived from cultured ...of sequencing from respiratory secretions was higher than ...
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Polymorphism In Growth Hormone Gene Sequence From Microminipig (MMP) With Direct Sequencing PCR
... Abstract: Pig Growth Hormone regulates growth, development and various metabolic activities in body. It releases from anterior pituatory gland of hypothalamus region of brain. Till now the sequence variation for GH has ...
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Detection of rubella virus gene sequences by enzymatic amplification and direct sequencing of amplified DNA
... PCR was done in 100-,ul reaction mixtures containing as target cDNA reverse transcribed from RNA extracted from purified stock RV 100 ,ul lanes 1 and 2, total cytoplasmic RNA 1 pug extra[r] ...
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Evaluation of multiplex ARMS-PCR for detection of Helicobacter pylori mutations conferring resistance to clarithromycin and levofloxacin
... our assay is based on a multiplex PCR format, it has inherent capacity for expansion to detect other antibiotic resistant H. pylori, by incorporating ARMS-PCR primers for other clinically significant mutations with ...
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Detection of R882 Mutations in DNMT3A Gene in Acute Myeloid Leukemia: A Method Comparison Study
... and direct sequencing, a dilution series of DNA from patients with DNMT3A R882H mutation was used and mixed with wild-type ...Both direct sequencing and HRM analysis were performed on serially ...
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C26232T Mutation in Nsun7 Gene and Reduce Sperm Motility in Asthenoteratospermic Men
... 5. Direct sequencing of exon7 of the Nsun7 gene in asthenoteratospermic men: (A), The wild type sequence showing ‘C’ at the nucleotide position 26232 in the Nsun7 gene; (B), Sequence of a ...
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Rapid Detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum Organisms in Genitourinary Samples by PCR Microtiter Plate Hybridization Assay
... the direct sequencing method, because there was no need to subclone the amplified products when multiple strains of mycoplasmas or ureaplasmas were present in clinical speci- ...
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Stock Structure and Homing Fidelity in Gulf of Mexico Sturgeon (Acipenser oxyrinchus desotoi) Based on Restriction Fragment Length Polymorphism and Sequence Analyses of Mitochondrial DNA
... Population structure: RFLP analysis of the entire mtDNA genome and direct sequencing analysis of the mtDNA control region provided congruent results and indicated extensiv[r] ...
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Original Article ROS1 gene rearrangement and clinicopathological characteristics in Chinese NSCLC patients
... and direct sequencing technique, we iden- tified nine cases with CD74-ROS1, seven with SLC34A2-ROS1, eight with SDC4-ROS1 and seven with TPM3-ROS1 fusion in the 31 ROS1- fusion positive NSCLC ...
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Molecular basis of the human dihydropyrimidine dehydrogenase deficiency and 5 fluorouracil toxicity
... activity. Direct sequencing of lymphocyte DNA from these subjects revealed the presence of a G to A point mutation at the 5'-splicing site consensus sequence (GT to AT) that leads to skipping of the entire ...
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Dynamic analysis of heterogeneous hepatitis C virus populations by direct solid phase sequencing
... somewhat higher than determined. More frequent sampling and shorter time intervals would be necessary to establish the actual mutation rate or change in virus population constitution in a patient over time. It must be ...
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Direct Whole Genome Sequencing of Sputum Accurately Identifies Drug Resistant Mycobacterium tuberculosis Faster than MGIT Culture Sequencing
... MGIT sequencing could still be the best possible workaround (15). Direct sequencing of ...pathogen sequencing, this could be offset, as dem- onstrated in our study, by only enriching areas of ...
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Expression and Characterization of Six Mutations in the Protoporphyrinogen oxidase gene among Finnish Variegate Porphyria Patients
... by direct sequencing of the patients’ genomic ...by sequencing the re- verse transcriptase polymerase chain reaction (RT-PCR) product amplified from total RNA extracted from the pa- tients’ ...
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Mutations in the histone methyltransferase gene KMT2B cause complex early onset dystonia
... Direct sequencing was undertaken to (i) screen the entire coding region of KMT2B for 13 cases from the GOS-ICH cohort (Supplementary Table 1), (ii) confirm variants identified on next generation ...
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