Discordant results
Molecular characterization of Mycobacterium avium complex isolates giving discordant results in AccuProbe tests by PCR restriction enzyme analysis, 16S rRNA gene sequencing, and DT1 DT6 PCR
... A detailed analysis of all DT1-positive isolates (14 discrep- ant strains plus 5 M. intracellulare strains) was performed by PRA, a method which was recently reported to be useful in identifying several mycobacterial ...
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Detection of Human Papillomavirus DNA in Urine Specimens from Human Immunodeficiency Virus Positive Women
... the results between the samples was compared, the results of the test with urine were not statistically different from those of tests with cer- vical swabs for the detection of any high-risk or low-risk HPV ...
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Safety and Immunogenicity of a Meningococcal Quadrivalent Conjugate Vaccine in Five- to Eight-Year-Old Saudi Arabian Children Previously Vaccinated with Two Doses of a Meningococcal Quadrivalent Polysaccharide Vaccine
... These discordant results between the two assays for sero- group A suggest that prior vaccination had an adverse effect on anticapsular antibody functional activity; that is, on average, twice the ...
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Performance Characteristics of FilmArray Respiratory Panel v1 7 for Detection of Adenovirus in a Large Cohort of Pediatric Nasopharyngeal Samples: One Test May Not Fit All
... ProAdeno PCR assay. The Prodessa ProAdeno ⫹ assay (ProAdeno PCR assay; Hologic/Gen-Probe, San Diego, CA) is an FDA-cleared, singleplex, real-time PCR assay. For a subset of specimens with discordant results ...
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Screening NK , B and T cell phenotype and function in patients suffering from Chronic Fatigue Syndrome
... with discordant results: CFS and control indi- viduals had similar expression of the T-cell immunosenes- cence marker CD57 [46] in CD4 and CD8 T cells, while differences were observed in the expression of ...
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Use of Amplification Refractory Mutation System PCR Assay as a Simple and Effective Tool To Detect HIV 1 Drug Resistance Mutations
... revealed discordant results for the different muta- tions; some were identified by ARMS-PCR as positive results whereas they were negative on sequence ...few results that were false negatives ...
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Performance of a nonisotopic DNA probe for detection of Chlamydia trachomatis in urogenital specimens
... After the resolution of discordant results, sensitivity, specificity, and predictive values of a positive and a negative result were calculated for both cell culture and GP Table 4 on th[r] ...
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Oligonucleotide Chip for Detection of Lamivudine Resistant Hepatitis B Virus
... the results of the oligonucleotide chip assay and those of PCR-RFLP analysis was ...with discordant results showed that the PCR-RFLP and sequencing methods could detect minority populations in ...
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Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis
... The results shown in Table 3 indicate that the Western blot assay and FTA-ABS DS were in close agreement, except for the following discordant results: the Western blot assay detected all[r] ...
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Detection of Immunoglobulin G Antibodies to Neospora caninum in Humans: High Seropositivity Rates in Patients Who Are Infected by Human Immunodeficiency Virus or Have Neurological Disorders
... concordant results or discordant results for IgG ...concordant results (ELISA positive and IFAT posi- tive) recognized at least one of two (29- and 35-kDa) immuno- dominant antigens of ...with ...
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Mycobacterium tuberculosis Strains with Highly Discordant Rifampin Susceptibility Test Results
... The coordinating SRL in Antwerp, Belgium, constituted a panel of 19 M. tuberculosis strains isolated from retreatment cases (Table 1), selected either on the basis of discordant results in earlier PT or ...
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Multiplex 5′ Nuclease Quantitative Real Time PCR for Clinical Diagnosis of Malaria and Species Level Identification and Epidemiologic Evaluation of Malaria Causing Parasites, Including Plasmodium knowlesi
... Molecular diagnosis of malaria offers many potential advantages over microscopy, including identification of malaria to the spe- cies level in an era with few experienced microscopists. We developed high-throughput ...
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New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a
... the results showed concordant results for 539 samples ...with discordant results, the nucleotide sequences of the HCV isolates were determined in 23, and their genotypes were determined by ...
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Comparison of Premier CAMPY Enzyme Immunoassay (EIA), ProSpecT Campylobacter EIA, and ImmunoCard STAT! CAMPY Tests with Culture for Laboratory Diagnosis of Campylobacter Enteric Infections
... gave discordant results by culture and any of the three EIAs were sent to the NYSDOH for arbitration by per- forming an in-house multiplex real-time PCR assay that was developed and validated for detecting ...
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Massively Parallel Sequencing (MPS) of Cell-Free Fetal DNA (cffDNA) for Trisomies 21, 18, and 13 in Twin Pregnancies
... test results, acatastatic sonographic signs, family genetic history, maternal age, and history of acatastatic fetal preg- ...karyotype results or clinical examination and follow-up were excluded from this ...
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Diagnosing Genital Ulcer Disease in a Clinic for Sexually Transmitted Diseases in Amsterdam, The Netherlands
... When the ulcers were suspected to have been caused by T. pallidum (estimated at 10% of the patients), dark-field microscopy was performed. Clinical manage- ment and treatment schemes were registered, and the diagnosis ...
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Identification of various medically important Candida species in clinical specimens by PCR restriction enzyme analysis
... However, the occurrence of false-positive or false-negative PCR results must be investigated with a larger number of specimens from patients at risk of developing disseminated fungal infections. To validate our ...
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Evaluation of performance of the RIBA processor system for automated analysis of the strip immunoblot assay for detection of antibodies to hepatitis C virus
... the results consists of capturing strip images, locating bands, measuring the density of reflectance (DR) for each band, calculating relative intensity ...the results, and determining the number on each ...
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Evaluation of a Tetraplex Microsphere Assay for Bordetella pertussis Antibodies
... CVR results. To demonstrate that pertussis antibody results determined by MAID were accurate, the MAID and Focus ELISA procedures were used to measure pertussis an- tibodies in 20 sera supplied by the UCLA ...
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Identification of Two Novel Mycobacterium avium Allelic Variants in Pig and Human Isolates from Brazil by PCR Restriction Enzyme Analysis
... Our results are relevant because an increase in the occurrence of mycobacteriosis was observed in the last years in the southern region of Brazil, where the pig isolates were ...
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