Disulfide Bond
Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli
... the disulfide bonds was initially postu- lated and later ...necessiting disulfide isomerization ...of disulfide-bond dependent proteins in the ...
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The Putative Herpes Simplex Virus 1 Chaperone Protein UL32 Modulates Disulfide Bond Formation during Infection
... the disulfide bond status of viral proteins, including the viral protease ...by disulfide bonds (9) and that the UL6 portal ring contains intersubunit di- sulfide bonds ...how disulfide ...
11
Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin.
... intramolecular disulfide bond ...the disulfide-dependent folding of E2 occurs rapidly and appears to be complete upon cleavage of the precursor ...
9
ATP Is Required for Correct Folding and Disulfide Bond Formation of Rotavirus VP7
... correct disulfide bonds without the pres- ence of additional rotavirus ...correct disulfide bonds in the absence of additional rotavirus proteins, VP7 was cloned and expressed by an SFV expression ...
5
Carbohydrates Facilitate Correct Disulfide Bond Formation and Folding of Rotavirus VP7
... The fact that PDI only interacted with correctly N-linked glycosylated VP7 suggests that carbohydrates and PDI are closely associated with correct folding of the ER-resident VP7. The exact role of the N-linked ...
6
Baculovirus GP64 Disulfide Bonds: the Intermolecular Disulfide Bond of Autographa californica Multicapsid Nucleopolyhedrovirus GP64 Is Not Essential for Membrane Fusion and Virion Budding
... intermolecular disulfide bond ...intermolecular disulfide bond, suggesting that the GP64 trimers were relatively thermostable in the absence of the intermo- lecular disulfide ...
12
Enhanced production of a single domain antibody with an engineered stabilizing extra disulfide bond
... a disulfide bond mutant of a single domain antibody specific for the L1 antigen of the vaccinia virus led to approximately ...second disulfide bond yielded only slight increases in protein ...
8
Human Immunodeficiency Virus Type 1 Env with an Intersubunit Disulfide Bond Engages Coreceptors but Requires Bond Reduction after Engagement To Induce Fusion
... engineered disulfide bond between the gp120 and gp41 subunits (SOS-Env) was expressed on cell ...the disulfide bond intact, these cells did not fuse to target cells expressing CD4 and CCR5, ...
8
Evolution of the HIV 1 envelope glycoproteins with a disulfide bond between gp120 and gp41
... the disulfide bond is lost by the C605Y ...the disulfide bond can be partially overcome by acquisition of the compen- satory Q591L substitution, as exemplified by two virus cultures that ...
11
The Fusion-Controlling Disulfide Bond Isomerase in Retrovirus Env Is Triggered by Protein Destabilization
... SU-TM disulfide bond isomerization in Env and facilitated virus-mediated cell-cell fusion and ...intersubunit disulfide bond, but require low pH in the endo- some, we suggest that the SU ...
8
A Prediction Method of Protein Disulfide Bond Based on Hybrid Strategy
... The important protein structure information is contained in protein evolution in- formation. Multiple protein sequences were compared, common conserved regions between these sequences with evolutionary relationships can ...
6
Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli
... ulfide bond formation in the periplasm [16] which trans- fers electrons to menaquinone or ...of disulfide bond formation are thought to act via an intermediary protein, protein disulfide iso- ...
9
Disulfide Bond Configuration of Human Cytomegalovirus Glycoprotein B
... no disulfide bond could be assigned to this cysteine ...the disulfide bond-dependent ...between disulfide bonds that are intermolecular and those that are intramolecular for cysteine ...
10
Disulfide Bond Formation Contributes to Herpes Simplex Virus Capsid Stability and Retention of Pentons
... that disulfide linkages are present in UL6, the HSV-1 portal protein ...the disulfide bonds detected between capsid proteins, those in UL6 can be detected in cells pretreated with NEM, suggesting that they ...
10
Native Conformation and Canonical Disulfide Bond Formation Are Interlinked Properties of HIV-1 Env Glycoproteins
... fide bond scrambling during ...epitopes, disulfide bond scrambling will inevitably affect antige- nicity and may also have a substantial influence on immunogenic- FIG 3 Protein processing in the ER ...
11
HcpE, a potential immuno-modulatory protein from Helicobacter pylori that is dependent on the Disulfide bond protein DsbHP
... Helicobacter pylori disulfide bond protein DsbHP: role in disulfice bond formation, redox stress resistance and production of Helicobacter cystein-rich protein HcpE. (Submitted)[r] ...
191
Disulfide bond structure of glycoprotein D of herpes simplex virus types 1 and 2.
... Cysteine mutants of herpes simplex virus type 1 glycoprotein D exhibit temperature-sensitive properties in structure and function.. Glycoprotein D protects mice against lethal challenge [r] ...
18
Nonself Recognition Through Intermolecular Disulfide Bond Formation of Ribonucleotide Reductase in Neurospora
... disulfide bond formation. Sub- sequently, the active site disulfide bond is transferred to C754 and C759 of the C terminus region, which is then reduced by glutaredoxin or thioredoxin to regenerate the enzyme ...
12
Disulfide bond formation in the human immunodeficiency virus type 1 Nef protein.
... To evaluate the effect of wild-type and cysteine mutant ELI and LAI Nef proteins on virus replication, the CD4+ T-cell line Jurkat and primary peripheral blood mononuclear cells PBMCs we[r] ...
5
The structure of the Bach2 POZ-domain dimer reveals an intersubunit disulfide bond.
... disulfide bond. The intersubunit disulfide bond is present both in bacterially expressed Bach2 POZ domain in solution and in protein expressed in trans- fected eukaryotic ...
10