Disulfide bond-containing protein
Localization of the labile disulfide bond between SU and TM of the murine leukemia virus envelope protein complex to a highly conserved CWLC motif in SU that resembles the active-site sequence of thiol-disulfide exchange enzymes.
... known protein sequences. A major class of proteins containing such a sequence consists of the thiol-disulfide exchange enzymes, such as DsbA, DsbB, thiore- doxin, and protein disulfide ...
5
Human Immunodeficiency Virus Type 1 Env with an Intersubunit Disulfide Bond Engages Coreceptors but Requires Bond Reduction after Engagement To Induce Fusion
... samples containing equal amounts of total cellular ...with protein A-agarose beads for 4 h at 4°C, and then the beads were washed extensively and boiled in 2⫻ SDS-PAGE sample loading buffer with and without ...
8
The Fusion-Controlling Disulfide Bond Isomerase in Retrovirus Env Is Triggered by Protein Destabilization
... SU-TM disulfide bond isomerization and enhance the fusion activity of ...SU-TM disulfide bond in Mo-MLV Env indicated that the reaction could be triggered by heat (48 and 53°C) or 2 M urea ...
8
The Putative Herpes Simplex Virus 1 Chaperone Protein UL32 Modulates Disulfide Bond Formation during Infection
... quired viral gene products, six of which are transiently or stably associated with capsids. The UL6 portal ring is found at a unique vertex on the procapsid and acts as a channel through which DNA is taken up (11–15). ...
11
Disulfide Bond Formation in the Herpes Simplex Virus 1 UL6 Protein Is Required for Portal Ring Formation and Genome Encapsidation
... this region were unable to form rings; instead, L429E L436E and D-LZ formed polymorphic aggregates. In the context of viral infection, leucine zipper mutants as well as C166A and C254A assemble B capsids that contain ...
9
Role of Conserved Cysteines in the Alphavirus E3 Protein
... binding protein and the E1 fusion ...precursor containing E3 and E2 ...E3 protein in Sindbis virus contains one disulfide bond between residues Cys19 and ...nested disulfide ...
8
Disulfide bonds of herpes simplex virus type 2 glycoprotein gB.
... secreted protein, which contains virtually the entire 702-amino-acid res- idue extracellular domain of HSV gB2, was purified by a procedure which consisted of chromatographic steps on S-Sepharose and Fractogel ...
9
Functional Implications of the Human T-Lymphotropic Virus Type 1 Transmembrane Glycoprotein Helical Hairpin Structure
... the disulfide- bonded ...the disulfide-bonded loop of the TM ...the disulfide bridge ...of protein backbones by restricting rotation about the N-C␣ bond (46), led to the complete ...
8
Reversible Inhibition of Fusion Activity of a Paramyxovirus Fusion Protein by an Engineered Disulfide Bond in the Membrane-Proximal External Region
... FIG 4 Reduction of disulfide bond in F T481C/T482C by TCEP restores fusion. (A) Chicken RBCs labeled with the lipidic dye R-18 (red) or the aqueous dye Calcein-AM (green) were bound to CV1 cells transfected ...
5
Disulfide Bond Configuration of Human Cytomegalovirus Glycoprotein B
... tag was constructed by PCR amplification using the following primer pair: 5⬘- CGG GAT CCG ACG AAC ATG GAA-3⬘ and 5⬘-GCT CTA GAA TTA TGA TGA TGA TGA TGA TGG GGG TTT TTG AGG AAG-3⬘ (accession number X04606). This fragment ...
10
HcpE, a potential immuno-modulatory protein from Helicobacter pylori that is dependent on the Disulfide bond protein DsbHP
... the Sec secretion signal due to the fact that they contain a positively charged N-region, a hydrophobic h-region as well as a short polar C-region that containes the cleavage site. The Tat signal sequence is recognized ...
191
Pre-expression of a sulfhydryl oxidase significantly increases the yields of eukaryotic disulfide bond containing proteins expressed in the cytoplasm of E.coli
... few disulfide bonds ...of disulfide bonds ...active protein produced up to 1000-fold over the wild-type ...of disulfide bonds is more effec- tive than the removal of the reducing pathways for ...
13
Magnetic nanoparticles containing labeling reagents for cell surface mapping
... affecting protein structure is another challenge in the labeling approaches to study ...the protein/peptide reactive group could help to release the biotinylated proteins/peptides from immobilized avidin ...
122
Carbohydrates Facilitate Correct Disulfide Bond Formation and Folding of Rotavirus VP7
... whereas disulfide bond formation is ...a protein with a molecular mass of around 40 kDa. This protein most likely represents VP6 (41 kDa), the major inner capsid protein of rotavirus ...
6
Binding of Host Cell Surface Protein Disulfide Isomerase by Anaplasma phagocytophilum Asp14 Enables Pathogen Infection
... antibodies were BD34 (BD Biosciences), 2B1 (ThermoFisher Scientific, Rockford, IL), anti-PDI antibody (MilliporeSigma, St. Louis, MO), anti-  -actin antibody (Santa Cruz Biotechnology, Dallas, TX), anti-GAPDH ...
16
Native Conformation and Canonical Disulfide Bond Formation Are Interlinked Properties of HIV-1 Env Glycoproteins
... by disulfide bond scram- bling (6, 23, 24, ...by disulfide bond scrambling, we find that aberrant disul- fide bonds are present in a substantial proportion of nonnative gp140 proteins ...
11
The Structure of G4, the Poxvirus Disulfide Oxidoreductase Essential for Virus Maturation and Infectivity
... X-ray data were collected (Table 1) and then integrated and scaled using HKL2000 (30). Five of the nine selenium positions were found by SOLVE (44) using the two datasets from the same crystal. Density modification and ...
8
Zika Virus Envelope Protein Forms Disulfide Bond-dependent Polymers in Connection with a Persistent ER Stress in A549 Cells
... and protein load in the ER lumen are biological reporters of ER stress, influencing UPR transduction and subsequent cell ...the protein (Oligomer, ...E protein fusion loop [24], revealed mainly a ...
14
ATP Is Required for Correct Folding and Disulfide Bond Formation of Rotavirus VP7
... secretory protein that undergoes a series of trimming and folding events during its transport through the secretory pathway, whereas rotavirus VP7 remains in the ER and only contains a few N-linked ...
5
The structure of the Bach2 POZ-domain dimer reveals an intersubunit disulfide bond.
... 2009). In addition to the classic POZ-domain dimerization interfaces, the mouse Bach1 POZ-domain structure revealed an interaction between the N-terminal six residues of one chain and the very C-terminal residues of the ...
10