Disulfide bond formation
Disulfide Bond Formation in the Herpes Simplex Virus 1 UL6 Protein Is Required for Portal Ring Formation and Genome Encapsidation
... the formation of disulfide bonds that could occur upon cell lysis and exposure to oxidizing ...the disulfide bonds in these capsids have already formed at the time of ...allows disulfide ...
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Disruption of reducing pathways is not essential for efficient disulfide bond formation in the cytoplasm of E. coli
... To further characterize the system, PhoA from all four expression conditions was purified by immobilised metal affinity chromatography. This one step purification resulted in highly purified protein (Figure 2A). Reduced ...
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Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin.
... oligomer formation, the rate of stable E1E2 complex formation, which is low, may be limited by the rate of HCV E1 and/or E2 ...intramolecular disulfide bond ...the disulfide-dependent ...
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Carbohydrates Facilitate Correct Disulfide Bond Formation and Folding of Rotavirus VP7
... interdisulfide bond aggregation. While formation of VP7 aggre- gates could be prevented under reducing conditions, they reoccurred in less than 30 min after a shift to an oxidizing ...protein ...
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ATP Is Required for Correct Folding and Disulfide Bond Formation of Rotavirus VP7
... In summary, we report for the first time that metabolic energy is required to ensure the correct folding and disulfide bond formation of ER-associated proteins, such as rotavirus VP7. We have also ...
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Disulfide Bond Formation Contributes to Herpes Simplex Virus Capsid Stability and Retention of Pentons
... that disulfide linkages are present in UL6, the HSV-1 portal protein ...the disulfide bonds detected between capsid proteins, those in UL6 can be detected in cells pretreated with NEM, suggesting that they ...
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Disulfide bond formation is a determinant of glycosylation site usage in the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus.
... Intramolecular disulfide bond formation might be such a folding ...6C. Formation of the intramolec- ular disulfide bond would serve to sequester the site, prevent- ing its usage ...
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The Putative Herpes Simplex Virus 1 Chaperone Protein UL32 Modulates Disulfide Bond Formation during Infection
... the disulfide bond status of viral proteins, including the viral protease ...by disulfide bonds (9) and that the UL6 portal ring contains intersubunit di- sulfide bonds ...how disulfide ...
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Imbalance of heterologous protein folding and disulfide bond formation rates yields runaway oxidative stress
... and disulfide bond formation, all with a layer of quality control at key check ...while disulfide bonds are ...[8]. Disulfide bond forma- tion requires the removal of electrons ...
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Antifungal Activity of the Enterococcus faecalis Peptide EntV Requires Protease Cleavage and Disulfide Bond Formation
... biofilm formation by inhib- iting hyphal ...disulfide bond that forms within EntV is neces- sary for antifungal ...this bond by chemical treatment or genetic modification rendered EntV inactive against ...
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Native Conformation and Canonical Disulfide Bond Formation Are Interlinked Properties of HIV-1 Env Glycoproteins
... aberrant) disulfide bonds can indeed be present in HIV-1 Env ...The formation of aberrant inter- molecular disulfide bonds causes the cross-linking of Env proteins into dimers and aggregates (31, ...
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Intracellular manipulation of disulfide bond formation in rotavirus proteins during assembly.
... To further explore the effects of DPT treatment of rotavirus-infected cells on individual rotavirus proteins, radiolabeled RRV-infected MA104 cell lysates were produced by adding 1 mM DP[r] ...
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Addition of high-mannose sugars must precede disulfide bond formation for proper folding of Sendai virus glycoproteins.
... If correct disulfides are essential for the formation of native protein structures, we would predict that i the reduction of disulfide bonds would abolish the native immunoreactivities, [r] ...
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Nonself Recognition Through Intermolecular Disulfide Bond Formation of Ribonucleotide Reductase in Neurospora
... H YPHAL fusion between conspecific fungi can result in the formation of heterokaryons: cells with more than one nuclear type. Growth of heterokaryotic cells is governed by nonself recognition loci (Glass and Kaneko ...
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Disulfide bond formation in the human immunodeficiency virus type 1 Nef protein.
... To evaluate the effect of wild-type and cysteine mutant ELI and LAI Nef proteins on virus replication, the CD4+ T-cell line Jurkat and primary peripheral blood mononuclear cells PBMCs we[r] ...
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A Conformational Change of C Fragment of Tetanus Neurotoxin Reduces Its Ganglioside-Binding Activity but Does Not Destroy Its Immunogenicity
... from disulfide bond formation did not destroy TeNT-Hc immunogenicity as a potent vaccine ...by disulfide bond formation, which did not affect the key amino acids to elicit strong ...
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CMD: a database to store the bonding states of cysteine motifs with secondary structures
... cysteine/ disulfide bond formation and its motif sequence compo- sition analysis by providing (1) a query tool for cysteine motifs based upon a comprehensive cysteine motif database curated from PDB ...
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Structure, mechanism and regulation of the clathrin coated vesicle and yeast vacuolar H(+) ATPases
... of disulfide bond formation in the regulation of vacuolar pH in the Golgi apparatus (Liu et ...inhibitory disulfide bond at the active site would be predicted to give rise to such a ...
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A Prediction Method of Protein Disulfide Bond Based on Hybrid Strategy
... structures, Disulfide bonds to maintain a stable three-dimensional structure of the protein and to remain active and function of the protein has a very im- portant ...The formation of disulfide ...
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Strategies for successful recombinant expression of disulfide bond-dependent proteins in Escherichia coli
... the formation of disulfide bonds for reaching its native structure can be produced in a functional form in the ...of disulfide bonds and native, func- tional structures was exploited by Beckwith and ...
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