DMEM/F12
Original Article Influence of different culture media on isolation of murine BMSCs
... α-MEM, DMEM and DMEM/F12, each was supplemented with 10% ...and DMEM media but just a few in DMEM/F12 medium at 5 days of ...in DMEM medium and there was no living cell in ...
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Human uterine leiomyoma-derived fibroblasts stimulate uterine leiomyoma cell proliferation and collagen type I production, and activate RTKs and TGF beta receptor signaling in coculture
... Primary cultured fibroblasts were taken from leiomyoma tissue samples obtained from women undergoing hyster- ectomy at Duke University Medical Center, in accordance with guidelines by the NIH Office of Human Subjects ...
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Endometrial mesenchymal stem cells isolated from menstrual blood repaired epirubicin-induced damage to human ovarian granulosa cells by inhibiting the expression of Gadd45b in cell cycle pathway
... assays; DMEM/ F12: Dulbecco ’ s modified Eagle ’ s medium; E2: Estradiol; ELISA: Enzyme-linked immunosorbent assay; FSHR: Follicle-stimulating hormone receptors; Gadd45: Growth arrest and DNA damage ...
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Secreted herpes simplex virus-2 glycoprotein G alters thermal pain sensitivity by modifying NGF effects on TRPV1
... Abbreviations: CGRP, Calcitonin gene-related peptide; DMEM-F12, Dulbecco ’ s modified Eagle medium with nutrient mixture F-12; FNE, Free nerve endings; HEPES, (4-(2-Hydroxyethyl)-1-piper[r] ...
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A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering
... AV: Aloe vera ; BCC: Basal cell carcinoma; DAPI: 4,6-diamidino-2-phenylindole; DMEM-F12: Dulbecco ’ s modified eagle medium; DMSO: Dimethyl sulfoxide; ECM: Extracellular matrix; ESM: Egg[r] ...
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In vitro induction of human embryonal carcinoma differentiation by a crude extract of Rhazya stricta
... Abbreviations DMEM/F12: Dulbeccoʼs Modified Eagle Medium: Nutrient Mixture F-12; DMSO: Dimethyl sulfoxide; EC: Embryonal carcinoma; ES: Embryonic stem; FBS: Fetal bovine serum; GC-MS: Ga[r] ...
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Influence of erythropoietin on microvesicles derived from mesenchymal stem cells protecting renal function of chronic kidney disease
... The mice primary bone marrow mesenchymal stem cells (BM-MSCs) were obtained from four-week-old male C57BL/6 mice, cultured and characterized as previ- ously described [20]. The extracted MSCs were cultured to the P3 ...
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Key genes and integrated modules in hematopoietic differentiation of human embryonic stem cells: a comprehensive bioinformatic analysis
... Before differentiation, H9 cell media was changed from E8 medium to Dulbecco’s modified Eagle medium/ nutrient mixture F-12 (DMEM/F12) complete medium prior to co-culture with irradiated (30 Gy) mouse em- ...
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Characterisation of feline renal cortical fibroblast cultures and their transcriptional response to transforming growth factor beta 1
... CKD: Chronic kidney disease; CKD-FCF: Feline cortical fibroblasts from cats with CKD; CTGF: Connective tissue growth factor; DMEM/F12: Dulbecco ’ s modified Eagle medium: Nutrient mixtur[r] ...
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Original Article Prognostic value of Thy1 in non-small cell lung cancer: a RNA-Seq transcriptome analysis
... sphere 95D cells were cultured under the stem cell conditions: serum-free DMEM/F12 contain- ing 0.4% BSA (sigma), 20 ng/ml basic fibroblast growth factor (bFGF) (Peprotech Rocky Hill, NJ, USA), 20 ng/ml ...
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Repair of human periodontal bone defects by autologous grafting stem cells derived from inflammatory dental pulp tissues
... Inflamed pulps from two patients were extirpated and placed in D-Hank’s solution. A routine RCT was per- formed. Inflamed pulps were then quickly placed in cul- ture medium for cell isolation. Each sample was first ...
9
Original Article Screening flavonoids and their synthetic analogs to target liver cancer stem-like cells
... liver cancer stem-like cells (LCSLCs) play key roles in individual programs of liver cancer clini- cal treatment. At present, there are several methods for separating and identifying LCSLCs including serum-free ...
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In Vitro Differentiation of Neural Stem Cells into Noradrenergic-Like Cells
... Medium F12 (DMEM /F12) (Gibco, USA) with 2% B-27 serum- free supplement (Gibco, USA), 2 mM L- glutamine (Gibco, USA), 1% penicillin (Invitrogen, UK), and 1% streptomycin (Invitrogen, UK) for ...
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Intravenous multipotent adult progenitor cell therapy after traumatic brain injury: modulation of the resident microglia population
... (DMEM/ F12) supplemented with 10% FBS, 100units/ml penicil- lin, 100 g/ml streptomycin and 5 ng/ml of granulocyte macrophage colony stimulating factor (GM-CSF) (415- ML-010/CF; R&D Systems, Minneapolis, ...
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Enteric nervous system abnormalities are present in human necrotizing enterocolitis: potential neurotransplantation therapy
... MA, USA) and cultured for an additional 12 hours. To detect nestin expression, neurospheres were incubated with mouse monoclonal antibody Rat-401 (1:100; De- velopmental Studies Hybridoma Bank of the University of Iowa, ...
13
The Cultivation of Human Granulosa Cells
... of DMEM/F12, 2 % FCS, ascorbic acid, dexamethasone, L-glu- tamine, gentamycine, penicillin, streptomycin and growth factors (EGF, ...containing DMEM/F12 medium supplemented with follicular ...
8
Production of interleukin 1 receptor antagonist by human articular chondrocytes
... bp = base pairs; C/EBP = CCAAT/enhancer binding protein; DMEM = Dubecco’s modified Eagle’s medium; ELISA = enzyme-linked immunosorbent assay; F12 = NUT.MIX.F-12 HAM Media; FCS = fetal ca[r] ...
8
Novel action of apolipoprotein E (ApoE): ApoE isoform specifically inhibits lipid-particle-mediated cholesterol release from neurons
... later, these neurons were washed three times with 1.5 ml of DMEM and incubated in DMEM containing reagents such as apoE3-HDL, apoE4-HDL, apoE3-EM, or apoE4- EM at various concentrations. Aliquots of 1.0 ml ...
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Computing Vertex PI, Omega and Sadhana Polynomials of F12(2n+1) Fullerenes
... Diudea, Computing PI and Omega Polynomials of an Infinite Family of Fullerenes, MATCH Commun. Ashrafi, Counting the number of hetero fullerenes, J[r] ...
6
Polymorphisms in Chlamydia trachomatis tryptophan synthase genes differentiate between genital and ocular isolates
... complete DMEM-10 as a control or with complete DMEM-10 further supple- mented with 5 ng/ml recombinant human IFN- γ with or without the addition of 50 µ M indole or 1 mg/ml ...
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