DNA amplification from the BAC clone
A BAC clone fingerprinting approach to the detection of human genome rearrangements
... sampling clone inserts is restriction fragment clone fingerprinting, which has been used by us and others to produce redundant clone maps of whole genomes ...Whole-genome clone mapping ...
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A BAC clone fingerprinting approach to the detection of human genome rearrangements
... sampling clone inserts is restriction fragment clone fingerprinting, which has been used by us and others to produce redundant clone maps of whole genomes ...Whole-genome clone mapping ...
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Rescue of a duck circovirus from an infectious DNA clone in ducklings
... the clone was used to confirm that the progeny virus was derived from the DNA clone and not from a contaminating ...rescued from ducklings infected with this mutated, full-length ...
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Microfluidic DNA amplification - a review
... To date, a single-phase containing PCR components is used to fill the channel in most continuous-flow microPCR devices, which can lead to carryover contamination between successive samples, adsorption at the surface, and ...
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Methods for microbial DNA extraction from soil for PCR amplification
... microorganisms from environmental samples is a fundamental obstacle to understanding microbial ecology and diversity ...of DNA-based techniques can overcome this limitation by allowing the fate of ...
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Isolation, amplification, and identification of ancient copepod DNA from lake sediments
... sediment from the center of the core should have eliminated this mode of ...Terrasovoje from a second species not currently found in the lake indicates that it is unlikely that contamination with recent ...
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Optimisation of DNA Extraction from Individual Sand Flies for PCR Amplification
... fly; DNA extraction; Calcium; PCR, lysis buffer, Lutzomyia ...of DNA from phlebotomines, including methods that eliminate DNA-associated proteins by using detergents and salts [7,8], or with ...
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Methods for the extraction, storage, amplification and sequencing of DNA from environmental samples
... article bau061. Schoch CL, Seifert KA, Huhndorf S, Robert V, Spouge JL, Levesque CA, Chen W, Bolchacova E, Voigt K, Crous PW, Miller AN, Wingfield MJ, Aime MC, An KD, Bai FY, Barreto RW, Begerow D, Bergeron MJ, Blackwell ...
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A Gossypium BAC clone contains key repeat components distinguishing sub-genome of allotetraploidy cottons
... repetitive DNA sequences and their evolu- tion in polyploidy ...repeats-rich BAC clone combining FISH verification with sequence analysis, and identified the key elements resulting in specific FISH ...
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Comparison of DNA Amplification, mRNA Amplification, and DNA Hybridization Techniques for Detection of Cytomegalovirus in Bone Marrow Transplant Recipients
... assay, DNA hybridization is followed by signal amplification and chemiluminescence ...CMV DNA test panel as ...571,514 DNA copies per ml); this was altered to ...571,514 DNA copies per ...
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Amplification of Borrelia burgdorferi DNA in skin biopsies from patients with Lyme disease
... To determine whether the polymerase chain reaction could contribute to a better diagnosis of Lyme disease, skin biopsy samples from patients suffering from erythema chronicum migrans or [r] ...
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Amplification and characterization of herpesvirus DNA in cerebrospinal fluid from patients with acute encephalitis
... A single pair of oligonucleotide primers selected within a highly conserved region of the DNA polymerase gene of the herpesviruses was designed to amplify related viral genomes, i.e., he[r] ...
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Dichlorvos exposure impedes extraction and amplification of DNA from insects in museum collections
... insects from natural history ...the DNA of the specimens in the ...on DNA of insects (Musca domestica) by extracting and amplifying DNA from specimens exposed to insecticides in two ...
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Strategies for the Extraction, Purification and Amplification of Metagenomic DNA from Soil Growing Sugarcane
... acids from environmental samples have proven to be po- werful tools for comparing and for exploring the ecology and metabolic profiling of complex environmental mi- crobial communities, as well as for identifying ...
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DNA fingerprinting by infrequent restriction site amplification
... of DNA from Neisseria meningitidis and Staphylococcus epidermidis isolates by using identical parame- ters suggests that IRS-PCR will be useful with these species as well (data not ...
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DNA amplification on chromosome 20q13 is commonly detected in
... It is more likely, however, that the patients exam- ined in the current study, the majority of whom were diagnosed at an early stage, already have a good prog- nosis and that a relatively small number of disease ...
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Evaluation-independent system for DNA section amplification
... differs from conventional systems in that it uses a side‑illumination optical detec‑ tion system and a temperature adjustment coefficient for DNA ...
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Integrated DNA extraction and amplification on a microfluidic device
... samples from both suspect and victims which were subjected to analysis using the integrated microfluidic ...system. DNA extraction and pre-concentration was performed ‘off-chip’ using commercial ...The ...
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A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchange
... of BAC/BIBAC clones of libraries originating from organisms with low GC con- tent results in a few large insert bands per clone when viewed on a CHEF ...for BAC/BIBAC clones that originate ...
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Design and validation of a pericentromeric BAC clone set aimed at improving diagnosis and phenotype prediction of supernumerary marker chromosomes
... Apart from ad hoc combinations of different methods, performed when requested, FISH analysis is currently the only available technique for analyzing sSMCs in low or cryptic ...the BAC clones and prepare the ...
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