DNA aptamer
Development of a DNA Aptamer for Screening Neisseria meningitidis Serogroup B by Cell SELEX
... values have previously been reported for whole cells specific aptamers [24-26] . In the present work, eight specific sequences were initially chosen, of which the aptamers K3 and K4 showed the highest activity (28.3 ± ...
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Design and Characterization of DNA Aptamer for Breast Tumor Marker by an Advantageous Method
... ABSTRACT : Aptamers are agents able to bind tightly and selectively to disease markers, offering great potential for applications in disease diagnosis and therapy. Functionally they are ssDNA or RNA oligonucleotides ...
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<p>Selection and Characterization of a Novel DNA Aptamer, Apt-07S Specific to Hepatocellular Carcinoma Cells</p>
... However, RNA aptamers are easily degraded by RNase, which undoubtedly increases the dif fi culty of its screening and application. 37 By contrast, it has been gradually realized that DNA aptamer is more ...
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Development, screening, and analysis of DNA aptamer libraries potentially useful for diagnosis and passive immunity of arboviruses
... process. DNA supernate (25 μl per tube) was siphoned out of the hot tubes without removing the target-MBs before the tubes cooled significantly and the DNA was ...hot DNA was added to 100 μl of fresh ...
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Potential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
... the DNA aptamer as an alternative element for upstream preparation in food analysis specifically to separate and concentrate Salmonella ...The aptamer was modified with biotin at 5’ end to complement ...
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A Novel DNA Aptamer for Dual Targeting of Polymorphonuclear Myeloid-derived Suppressor Cells and Tumor Cells
... aptamer-injected and control mice, respectively. Tumor samples were frozen and processed for confocal fluorescence microscopy to detect Cy5-labeled aptamers. Single-cell suspensions were prepared from blood, lung, ...
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Robust, specific ratiometric biosensing using a copper-free clicked quantum dot-DNA aptamer sensor
... CFCC and its use in label- and label-free- detection of DNA and protein targets via target binding induced changes to the QD sensitized dye FRET signals. First, a multi-functional ligand, containing a ...
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DNA Aptamer Selected against Pancreatic Ductal Adenocarcinoma for in vivo Imaging and Clinical Tissue Recognition
... Cy5-labeled aptamer or library, ...of aptamer XQ-2d was also ex- pressed in patients with ...Therefore, aptamer XQ-2d holds potential as an effective molecular di- agnostic reagent for recognizing ...
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Beyer, Stefan (2006): DNA-based molecular templates and devices. Dissertation, LMU München: Fakultät für Physik
... of DNA which can be controllably switched between different ...specific DNA operator strands [4– ...for DNA nanodevices can also be utilized for the construction of a switchable DNA ...
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Aptamers-the Alternative To Antibodies Conjugated Gold Nanoparticles For Detection Of Staphylococcus Aureus
... whereas DNA aptamer are extremely stable in both hydrated and lyophilized form ...of aptamer as an alternative to antibody conjugated gold nanoparticles for detection of ...
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Electrochemical Detection of VEGF165 Lung Cancer Marker Based on Au-Pd Alloy Assisted Aptasenor
... employed aptamer [32, 33]. In particular, the distinct fold of this DNA aptamer inspired us to develop a sensor which could directly transform the change of conformation induced by the target into ...
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Combining functionalised nanoparticles and SERS for the detection of DNA relating to disease
... specific DNA sequences by using a biomolecular recognition event to ‘turn on’ a SERS response through a controlled assembly process of the DNA functionalised ...prepared DNA aptamer ...
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Anti-FGFR1 aptamer-tagged superparamagnetic conjugates for anticancer hyperthermia therapy
... the aptamer is only used as a targeting ...a DNA aptamer that specifically binds to the extracellular domain of recombinant fibroblast growth factor receptor type-1 (FGFR1) with a nanomolar ...
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1 DNA Aptamers for Functionalisation of DNA
... of aptamer use in controlling DNA origami structural ...designing aptamer-based fluorescent reporters that function by switching structures from DNA/DNA duplex to DNA/target ...
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Theophylline IMAGEtags (Intracellular Multi Aptamer Genetic tags): the development and evaluation of an RNA reporter system based on the theophylline aptamer
... The DNA stain Hoechst 33258 binds to AT rich regions of double stranded DNA (dsDNA), whereby its fluorescence is ...A DNA aptamer was then selected for this derivative and binding to the ...
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RNA aptamers specifically interact with the prion protein PrP.
... crude protein extracts, brain homogenates from mice were assayed for PrP-RNA interactions in gel retardation assays. Brain homogenates from wild-type mice incubated with radio- labelled RNA aptamer Ap1 yielded a ...
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Aptamer-based sandwich-type biosensors
... developing aptamer pairs successfully ...of aptamer pairs for one protein target and two kinds of virus targets were reported based on the use of GO-SELEX so far ...for aptamer pair is multivalent ...
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Recent Microdevice-based Aptamer Sensors
... Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of 749. Hybridization Chain Reaction[r] ...
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Aptamer–drug conjugate: targeted delivery of doxorubicin in a HER3 aptamer-functionalized liposomal delivery system reduces cardiotoxicity
... the aptamer was incubated with proteins for 1 h at 25 ° C, shaking ...protein-bound aptamer–streptavidin complex was quantified by determining the absorbance at 450 nm using a SpectraMax Plus (Thermo Fisher ...
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APTAMER: A MIRACLE TOOL FOR TARGETED DRUG DELIVERY
... research. Aptamer-conjugated, multifunctional liposomes have been fabricated to encapsulate and deliver cisplatin to breast cancer cells ...multiple aptamer- receptor interactions, provides better binding ...
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