DNA-binding Affinity and Subcellular Localization of
Dynamic changes to survivin subcellular localization are initiated by DNA damage
... and DNA-PK) phosphorylate a variety of effector substrates that propagate DNA damage signals, ultimately resulting in various biological outputs influencing cell cycle arrest, transcription, DNA ...
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Accurate and sensitive quantification of protein-DNA binding affinity
... NRLB Model Construction for HT-SELEX Data. NRLB models were built for 30 of the 35 HT-SELEX datasets used in the DeepBind study (30) (European Nu- cleotide Archive identifiers ERP001824 and ERP001826). Of the five that ...
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Localization of DNA binding polyamides in living cells
... chromosomal DNA sequence-specifically and with high affinity would be important tools for gene ...of DNA in a sequence-specific fashion according to a set of pairing rules, and with affinities ...
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High affinity RNA binding by a hyperthermophilic single stranded DNA binding protein
... in DNA replication, recombination, and ...bind DNA, and typically oligomer- ise in solution to bring multiple OB fold domains together in the functional ...where DNA and RNA damage is a more frequent ...
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High affinity binding of MEF-2C correlates with DNA bending
... contrast, binding of MEF-2C to the MEF-4G,5C site led to a slight blue shift ...heterologous DNA also underwent a conformational change upon ...mutant DNA appears to be much less dramatic and of a ...
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The Oxidation State of [4Fe4S] Clusters Modulates the DNA-Binding Affinity of DNA Repair Proteins
... on DNA-modified electrodes is a diffusionless or a diffusive process, we conducted Randles-Sevcik analysis by measuring the peak current responses at various scan rates while holding the concentration of EndoIII ...
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High-Affinity Rb Binding, p53 Inhibition, Subcellular Localization, and Transformation by Wild-Type or Tumor-Derived Shortened Merkel Cell Polyomavirus Large T Antigens
... Consequently, it is thought that T-Ags encoded by Merkel cell polyomavirus (MCPyV), a virus integrated in ⬃90% of all Merkel cell carcinoma (MCC) cases, are major contributors to tumorigenesis. The MCPyV large T-Ag ...
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Probing the DNA-Binding Affinity and Specificity of Designed Zinc Finger Proteins
... site-specific DNA-binding domains based on the zinc finger architecture can be fused to an appropriate transcription effector domain to yield ‘‘custom’’ transcription factors suitable for the directed regu- ...
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Cell- and Virus-Mediated Regulation of the Barrier-to-Autointegration Factor's Phosphorylation State Controls Its DNA Binding, Dimerization, Subcellular Localization, and Antipoxviral Activity
... a DNA binding protein with important functions in mitosis, the antiviral response, and likely other cellular processes, it is nec- essary that BAF is tightly ...its DNA binding activity and ...
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Arginine methylation of the cellular nucleic acid binding protein does not affect its subcellular localization but impedes RNA binding
... acid binding protein (CNBP) contains seven zinc finger (ZF) repeats and an arginine and glycine (RG) rich sequence between the first and the second ...nuclear localization of ...the subcellular ...
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Studies on the binding affinity of anticancer drug mitoxantrone to chromatin, DNA and histone proteins
... higher affinity than free ...The binding of mitoxantrone to chromatin produces a very compact structure that prevents the release or extraction of histone proteins from such drug treated ...the ...
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Identification of a gC1q binding protein (gC1q R) on the surface of human neutrophils Subcellular localization and binding properties in comparison with the cC1q R
... mannose binding protein, surfactant protein-A, surfactant protein-D, or conglutinin under normal ionic conditions, suggesting a greater specificity for C1q than the "collectin" type receptor ...the binding ...
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Binding properties of avian myeloblastosis virus DNA polymerases to nucleic acid affinity columns.
... at 0.2 M KCI, approximately 20% of the enzyme also bound nonspecifically to blank cellulose with a broad peak of activity eluting between 0.30 and 0.6 M KCI.. Recovery of enzymatic activ[r] ...
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Study on Binding Affinity of a Glutathione S Transferase (GST) Fusion Protein to DNA Probe
... specific DNA-binding sequences, shown in the ...actual binding takes place. DNA-binding proteins may have one or more subdomains: one containing a conserve motif that contacts the ...
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Protein targeting to subcellular organelles via mRNA localization
... mRNA localization to mitochondria Proteins are imported from the cytoplasm into chloroplasts, mitochondria, and peroxisomes by the recognition of a transit peptide sequence, generally located at their N- terminus, ...
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Dynamic visualization of transcription and RNA subcellular localization in zebrafish
... ABSTRACT Live imaging of transcription and RNA dynamics has been successful in cultured cells and tissues of vertebrates but is challenging to accomplish in vivo. The zebrafish offers important advantages to study these ...
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Subcellular Localization in Bacteria: From EnvZ/OmpR to Transertion
... known binding sites within the chromosome, the localization pattern was not obviously due to OmpR binding at individual ...the DNA specific dye, ...
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Subcellular Localization of Survivin Determines Its Function in Cardiomyocytes
... the localization of SVV was able to depict its bioactivity in cardiomyocytes after MI, we expected that the presence of nuclear localization signal on NLS-SVV would localize SVV exclusively in the nucleus ...
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Membrane Binding and Subcellular Localization of Retroviral Gag Proteins Are Differentially Regulated by MA Interactions with Phosphatidylinositol (4,5) Bisphosphate and RNA
... intracellular localization via VLP endocytosis for these GagLZ proteins would have to be an MA-specific process rather than nonspecific ...membrane binding, HTLV-1 MA GagLZ and MLV MA GagLZ are able to bind ...
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The C-terminal extension of human telomerase reverse transcriptase is necessary for high affinity binding to telomeric DNA
... telomeric DNA, but the magnitude of this impact varies for primers with different 3 0 ...the DNA-RNA helix differentially with different DNA ...in DNA binding affinity, a function ...
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