DNA Cloning and analysis
Cloning of the DNA of mycobacterium tuberculosis and analysis of the expressed protein antigens
... Several non-i mrnu no logical teats useful for the diagnosis of tuberculosis are available: measurement of adenosine. deaminase (ADA) activity in pleural, peritoneal, [r] ...
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Cloning, sequencing, and functional analysis of a Marek's disease virus origin of DNA replication.
... Neither the replication origins of Epstein-Barr virus (oriP and oriLyt) nor those of cytomegalovirus contained sequences similar to the putative origin of serotype 2 MDV replication (1, [r] ...
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Virion DNA of ground squirrel hepatitis virus: structural analysis and molecular cloning.
... The structure of the encapsidated DNA genome of ground squirrel hepatitis virus (GSHV) has been examined by restriction endonuclease cleavage, nucleic acid hybridization, and molecular c[r] ...
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Repertoire cloning of lupus anti DNA autoantibodies
... *These data represent results of hybridization of membrane lifts of bacterial colonies with 150 to 250 colonies per plate for each V H gene probe, with subsequent stripping and reprobing with a constant region-specific ...
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Cloning and Functional Analysis of Kaposi’s Sarcoma-Associated Herpesvirus DNA Polymerase and Its Processivity Factor
... The DNA synthesis activities of Pol-8, PF-8, and Pol-8 and PF-8 together were compared on a 15% urea-polyacrylamide ...The DNA synthesis assay employed M13 ssDNA template with an annealed 59-end 32 ...
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Molecular cloning of proviral DNA and structural analysis of the transduced myc oncogene of avian oncovirus CMII.
... cloning of CMII proviral DNA, we compare its genetic structure and particularly its transforming gene with those of the other members of the MC29 subgroup, and we present evidence from n[r] ...
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Avian oncovirus MH2: molecular cloning of proviral DNA and structural analysis of viral RNA and protein.
... Avian acute leukemia viruses MC29 and MH2 share specific RNA sequences: evidence for a second class of transforming genes. Restriction[r] ...
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Cloning and DNA sequence analysis of the region containing attp of the temperate phage 4AR29 of Prevotella ruminicola AR29
... In summary, D N A encoding the integration site of 4AR29 was isolated, and appears to contain a module composed of an int gene, an ORF suitably located to be an xis gene, a[r] ...
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Cloning and Characterization of a Complex DNA Fingerprinting Probe for Candida parapsilosis
... 15-kb DNA fingerprinting probe, Cp3-13, was therefore isolated from a library of ...genomic DNA fragments. The efficacy of Cp3-13 for DNA fingerprinting was verified by a comparison of its clustering ...
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Investigating a Eukaryotic Genome: Cloning and Sequencing a Fragment of Yeast DNA
... of DNA fragments recovered is designed to gain some insight into the organization of eukaryotic ...bacterial cloning, and molecular ...bacterial cloning and cycle ...
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NEBuilder. HiFi DNA Assembly Master Mix/NEBuilder HiFi DNA Assembly Cloning Kit
... HiFi DNA Assembly Master Mix and NEBuilder ® HiFi DNA Assembly Cloning Kit are warranted to perform according to specifications stated on the certificate of ...
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Cloning and Expression Analysis of GmCYP78A5 Promoter
... technology. DNA sequence alignment indicated that the amplified fragment (1650bp) was ...Bioinformatics analysis showed that the GmCYP78A5 promoter contains a lot of inducible or tissue-specific expression ...
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Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad range PCR, cloning and sequencing
... derived DNA. Thus, the presence of P. acnes DNA in these patients is likely to be either disease specific or due to opportunistic colonization of the inflamed ...
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Directional cloning of DNA fragments using deoxyinosine-containing oligonucleotides and endonuclease V
... Germany). DNA concentrations were determined using a NanoDrop 2000 micro-volume UV-Vis spectrophotometer (Thermo Fisher Scientific, Schwerte, ...product analysis or purification, respectively, were prepared ...
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Molecular Cloning and Expression Analysis of PgLAC in Pomegranate
... DOI: 10.4236/ajmb.2018.83012 147 American Journal of Molecular Biology sequence, 5’ end specific primers LAC-5’GSP1, LAC-5’GSP2 and 3’ end specific primers LAC-3’GSP1, LAC-3’GSP2 were designed. LAC-5’GSP1/UPM Long were ...
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GENE CLONING AND RECOMBINANT DNA TECHNOLOGY
... Genetically modified rice carrying Xa21 gene were tested in the field and compared with wildtype rice plants. Wildtype rice GM Rice[r] ...
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LAB 7 DNA RESTRICTION for CLONING
... plasmid DNA is run on an agarose gel, many bands are usually ...the DNA that determines how quickly it can migrate through a gel in an electric ...plasmid DNA can take allows it to move through ...
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Cloning and characterization of complementary DNA for human tryptase
... Asp217 in the substrate binding pocket of human tryptase is consistent with a specificity for Arg and Lys residues at the site of cleavage (P1), whereas Glu245 is consistent with the kno[r] ...
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Cloning human telomeric DNA fragments into Saccharomyces
... Unequivocal proof that the cloned fragments represent sequences that start at an internal EcoRI site and end within the (TTAGGG)" repeat of a natural human telomere will require the [r] ...
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Molecular cloning and physical mapping of murine cytomegalovirus DNA.
... The results obtained after hybridiza- tion of 32P-labeled cloned fragments to Southern blots of MCMV DNA cleaved with HindIII, XbaI, EcoRI, BamHI, ApaI, ClaI, EcoRV, or KpnI allowed us t[r] ...
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