DNA dependent RNA polymerase modification and replacement
Evidence for a unique DNA-dependent RNA polymerase in cereal crops
... DNA-dependent RNA polymerase (Pol) II subunits within plants led to the emergence of RNA Pol IV and V complexes, each of which possess unique functions necessary for RNA-directed ...
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RNA-dependent DNA polymerase associated with equine infectious anemia virus.
... equine infectious anemia virus and an antigen ex- tracted from infected horse spleen. Physi- cochemical studies of equine infectious anemia virus[r] ...
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Purification and Characterization of a DNA-dependent RNA Polymerase from Vaccinia Virions*
... To determine the polypeptide composition of the vaccinia virus RNA polym- erase, fractions obtained during successive steps in purification and after glycerol gradie[r] ...
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Use of DNA, RNA, and Chimeric Templates by a Viral RNA-Dependent RNA Polymerase: Evolutionary Implications for the Transition from the RNA to the DNA World
... progenitor polymerase. Viral RNA-dependent RNA polymerases (RdRp) are expected to have properties comparable to those from this progenitor and therefore may offer insight into the common- ...
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RNA-Dependent DNA Polymerase Activity of RNA Tumor Viruses II. Directing Influence of RNA in the Reaction
... The polymerase catalyzes the synthesis of DNA in the presence of four deoxynucleoside triphosphates, Mg2+, and a variety of RNA templates including those isolated from avian myeloblastos[r] ...
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Crystal structures of the RNA-dependent RNA polymerase
... molecular replacement with the CNS package (28) using the unliganded 1b genotype polymerase struc- ture (8) (Protein Data Bank code 1C2P) as the search ...
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Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase
... disrupted, RNA replication was greatly reduced (18), whereas disruption of the putative minus-strand stem-loop structure was not correlated with effects on viral replication ...plus-strand RNA is involved ...
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RNA-dependent DNA polymerase activity of RNA tumor virus. VI. Processive mode of action of avian myeloblastosis virus polymerase.
... (A) Each polymerpair was incubated with polymerase separately; (B) both polymerpairs were incubated with polymerase together; (C) enzyme was incUbated with poly(I) *poly(dC) and poly(A) [r] ...
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DNA-dependent RNA polymerase subunits encoded within the vaccinia virus genome.
... Cell-free translation of early and late mRNAs selected by hybridization to cloned DNA fragments derived from the left 14 million to 72 million daltons of the vaccinia virus genome. Homol[r] ...
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Development of a Challenge-Protective Vaccine Concept by Modification of the Viral RNA-Dependent RNA Polymerase of Canine Distemper Virus
... Proliferation potencies of PHA-stimulated PBMCs from individual animals in the group primarily infected with rCDV 5804P (C) and in the groups infected with rCDV 5804P -L(CC EGFP C) (D) a[r] ...
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Antiviral Effects of a Transgenic RNA-Dependent RNA Polymerase
... Viral infections cause many chronic devastating diseases, and acute viral infections can be life threatening. Currently, vaccinations are the main strategy for preventing viral epidem- ics. Although vaccination regimens ...
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Binding and transcription of simian virus 40 DNA by DNA-dependent RNA polymerase from Escherichia coli.
... Specificity of initiation of transcription of simian virus 40 DNA I by Escherichia coli RNA polym- erase: identification and localization of five sites for initiation with [y-3P]ATP. Ani[r] ...
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DNA-Dependent RNA Polymerase Activity Associated with Subviral Particles of Polyhedral Cytoplasmic Dexoyribovirus
... b The standard assay procedure (see Materials and Methods) was performed by using 10 Mg of subviral protein per enzyme reaction. The specific activity of the control reaction was 1,575 p[r] ...
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Intramolecular and Intermolecular Uridylylation by Poliovirus RNA-Dependent RNA Polymerase
... 3D polymerase also catalyzes inter- and intramolecular uridylylation of 3D polymerase mol- ecules ...3D polymerase occurs by a single-step mechanism, as does the uridylylation of VPg, suggesting that ...
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Structural and Functional Characterization of Sapovirus RNA-Dependent RNA Polymerase
... the polymerase activity of sapovirus in vitro, a 3D pol domain encompassing the predicted sapovirus RNA polymerase motifs A to E was delineated in the polyprotein ...other RNA-dependent ...
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Vaccinia virus gene encoding a 30-kilodalton subunit of the viral DNA-dependent RNA polymerase.
... Homology between RNA polymerase of poxviruses, prokaryotes, and eukaryotes: nucle- otide sequence and transcription analysis of vaccinia virus genes encoding 147-kDa and 22-kDa subunits.[r] ...
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RNA-dependent DNA polymerase of an endogenous type C virus of mice: purification and partial characterization.
... purification and the specific activity of the final enzyme preparation were below those obtained in the purification of the DNA polymerase from avian sarcoma virus (ASV) (5, 9, 10), muri[r] ...
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Rational Attenuation of a Morbillivirus by Modulating the Activity of the RNA-Dependent RNA Polymerase
... Negative-strand RNA viruses encode a single RNA-dependent RNA polymerase (RdRp) which transcribes and replicates the ...resulting polymerase was autofluo- rescent, and its ...
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RNA-Dependent DNA Polymerase Activity of RNA Tumor Viruses I. Directing Influence of DNA in the Reaction
... After chromatography of the ammonium sulfate fraction on phospho- cellulose, there was no detectable activity with native DNA as primer; the activity with poly (I) poly (dC) was unchange[r] ...
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Norovirus Proteinase-Polymerase and Polymerase Are Both Active Forms of RNA-Dependent RNA Polymerase
... viral RNA-dependent RNA polymerase (RdRp) domains: ProPol (a precursor comprised of both the proteinase and polymerase) and Pol (the mature ...
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