DNA DNA hybridization
Detection of Corynebacterium kutscheri in animal tissues by DNA DNA hybridization
... kutscheri were isolated, radiolabeled with 32p, and used in DNA-DNA hybridization assays with blots of impression smears of animal tissues on membrane filters.. kutscheri probes did not [r] ...
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Characterization of Vibrio metschnikovii and Vibrio gazogenes by DNA DNA hybridization and phenotype
... gazogenes strains were tested at 25°C on Mueller-Hinton agar with 1.5% NaCl added, because they did not grow without the additional NaCl.. Sizes of zones of inhibition are given for V.[r] ...
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COMPARISON OF THE DNA OF SIX VICIA SPECIES BY THE METHOD OF DNA-DNA HYBRIDIZATION
... If the difference in DNA content per cell between two species were due to uneven multipli- cation of DNA messages (local multiplicity), more labeled DNA would he hybridi[r] ...
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PubMedCentral-PMC5168558.pdf
... Over the past two decades, root canal treatment failure has been attributed to the presence of E. faecalis (2, 33, 34). In this study, E. faecalis was detected at a low mean proportion (0.52±0.26; Fig. 1). Recently, ...
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Bacillus subtilis bacteriophages SP82, SPO1, and phie: a comparison of DNAs and of peptides synthesized during infection.
... The extent of relatedness of these three phages has been examined by means of DNA-DNA hybridization, by comparing the fragments produced by digestion of phage DNAs with four restriction [r] ...
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Distribution of Staphylococcus Species among Human Clinical Specimens and Emended Description ofStaphylococcus caprae
... The type strains used for DNA-DNA hybridization were as follows: S. aureus subsp. aureus ATCC 12600, S. capitis subsp. capitis ATCC 9121, S. cohnii subsp. cohnii ATCC 29974, S. epidermidis ATCC ...
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Clinical and epidemiological investigations of Acinetobacter genomospecies 3 in a neonatal intensive care unit
... by DNA-DNA hybridization showed that most of the isolates in our study belonged to the unnamed genomo- species 3, which is closely related to genomospecies 2 ...by DNA- DNA ...
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Integration of the Deoxyribonucleic Acid of Adenovirus Type 12 into the Deoxyribonucleic Acid of Baby Hamster Kidney Cells
... However, when the DNA of N-BHK-21 cells infected with 3HAdl2 is analyzed by zonal sedimentation in alkaline sucrose density gradients and DNA-DNA hybridization, it is found that the 3H l[r] ...
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Investigation of association of mycobacteria with inflammatory bowel disease by nucleic acid hybridization
... We report here the results of studies in which DNA-DNA hybridization techniques were used to characterize strain Linda and to search for mycobacterial DNA sequences in tissues obtained f[r] ...
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Identification of clinically relevant viridans group streptococci to the species level by PCR
... We finally investigated our strategy by testing 91 character- ized strains of viridans group streptococci. The PCR results confirmed the identification of 60 strains (10 S. gordonii, 10 S. mitis, 10 S. mutans, 10 S. ...
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Exclusion of bacteriophage T1 by bacteriophage lambda. II. Synthesis of T1-specific macromolecules under N-mediated excluding conditions.
... gene products data not DISCUSSION From DNA-DNA hybridization experiments with DNA extracted from excluding and control cultures labeled with [3H]thymidine by either prelabeling or contin[r] ...
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Supragingival plaque microbial analysis in reflection to caries experience
... “checkerboard DNA-DNA hybridization” based panel of caries-related bacteria, could reflect the caries experience in a manner similar to saliva samples analysed using a chair-side method in a previous ...
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Characterization of Aeromonas schubertii strains recently isolated from traumatic wound infections
... Their identification was confirmed by DNA-DNA hybridization to the Centers for Disease Control definition strain for group 12 ATCC 43700.. These strains were additionally evaluated for t[r] ...
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Amplified Fragment Length Polymorphism Analysis: the State of an Art
... one DNA hybridization group, while different strains within this group could be ...14 hybridization groups as well as four species not yet allocated to a hybridization group revealed a strong ...
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Hybridization Selection and In Vitro Translation of Autographa californica Nuclear Polyhedrosis Virus mRNA
... By hybridization selection of viral RNA to DNA fragments instead of intact AcMNPV DNA and in vitro translation of these RNAs, we demonstrated that the mRNA sequences directing the synthe[r] ...
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Genetic relatedness of equine herpesvirus types 1 and 3.
... Thus, the addition of the unlabeled second viral DNA EHV-3 to the hybridization reaction increased the concentration of sequences homologous to only a restricted portion of the probe DNA[r] ...
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Wild type and central DNA flap defective HIV 1 lentiviral vector genomes: intracellular visualization at ultrastructural resolution levels
... here, DNA hybridization signals remain on the cytoplasmic side of the nuclear membrane, with no overlap across the nuclear membrane, revealing that Flap defective DNA molecules have not initiated ...
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Transcription During the Development of Bacteriophage φ29: Definition of “Early” and “Late” φ29 Ribonucleic Acid
... Early e 029 RNA, made prior to the onset of phage deoxyribonucleic acid DNA replication and exclusively from the light L 029 DNA strand, has been shown by RNA-DNA hybridization-competiti[r] ...
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Chemiluminescent Detection of DNA Hybridization Based on Signal DNA Probe Modified with Gold and Cobalt Nanoparticles
... probe DNA solution and 200 μL of 1.0 × 10 -7 M single DNA solution were added to 1 mL of the prepared gold NPs ...signal DNA and probe DNA tagged with gold NPs and cobalt NPs was collected by ...
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Genetic relatedness and colinearity of genomes of equine herpesvirus types 1 and 3.
... The decisions to use only cloned viral DNA fragments as probes and to use a standardized set of stringent hybridization conditions which permitted detection of DNA sequences that display[r] ...
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