DNA End-Joining Repair
DNA repair and gene targeting in plant end-joining mutants
... to DNA damage, the Mre11-Rad50-Xrs2 (MRX) complex in yeast and its counterpart in mammals, called Mre11-Rad50-Nbs1 (MRN), function early as a key player in the DNA damage sensing, signaling and ...
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DNA damage response and repair in perspective: Aedes aegypti, Drosophila melanogaster and Homo sapiens
... the DNA damage response (DDR), which controls the lesion detection and DNA ...main repair pathways are base excision repair (BER), nucleotide excision repair (NER), mismatch ...
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Heat shock factor 1, an inhibitor of non-homologous end joining repair
... non-homologous end joining (NHEJ) repair activity was ...NHEJ repair activity and ultimately activated genomic instability after ionizing radiation (IR), which was similar to effects seen in ...
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Lig4 and Rad54 Are Required for Repair of DNA Double-Strand Breaks Induced by P-Element Excision in Drosophila
... the repair junctions revealed microhomology (2–8 bp)-dependent DSB repair in most ...for repair. Our data indicate the presence of efficient alternative end-joining mechanisms, which ...
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The Rag2 C Terminus Participates in Repair Pathway Choice in Vivo and Suppresses Lymphomagenesis
... on DNA repair genes and additional genes that their activity is still not fully elucidated but might be participating in DNA ...that end various established end-joining assays ...
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The histone deacetylase inhibitor PCI-24781 as a putative radiosensitizer in pediatric glioblastoma cell lines
... of DNA dam- age, among them double-strand breaks ...irreparable DNA damage, only one DSB is already potentially cytotoxic and can induce apoptosis in certain cell types ...and repair of these ...
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Original Article Blood-based DNA methylation of DNA repair genes in the non-homologous end-joining (NEHJ) pathway in patient with glioma
... A nested MSP approach was performed on the sodium-bisulfate-treated DNA samples to amplify the promoter region of the five repair genes. For each gene (LIG4, XRCC4, XRCC5, XRCC6, and XRCC7), four pairs of ...
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Double-Strand Break Repair by Non-homologous End-Joining
... The joining of protruding 5'-0H termini by human cell-free extracts was shown to be highly efficient and accurate, without loss or gain of nucleotides at the junction ...vitro end-joining studies, ...
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CRISPR/Cas9-Induced Double-Strand Break Repair in Arabidopsis Nonhomologous End-Joining Mutants
... -deficient repair junctions, although most junctions were repaired without an ...in repair products without insertions during Cas9-induced ...involving DNA polymerase u (McVey and Lee 2008; Koole et ...
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PubMedCentral-PMC5466332.pdf
... Previous biochemical studies have established that the polymerase domain of human Pol θ aligns substrates with long GC-rich microhomologies more efficiently than AT-rich micro- homologies and prefers using terminal ...
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Mismatch Tolerance by DNA Polymerase Pol4 in the Course of Nonhomologous End Joining in Saccharomyces cerevisiae
... can repair a DSB created in vitro on a plasmid by enzymatic restriction (O rr -W eaver and S zostak 1983; B oulton and J ackson ...plasmid DNA (Table 2) or KpnI-digested plas- mid DNA displaying ...
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SIRT1 inhibition impairs non-homologous end joining DNA damage repair by increasing Ku70 acetylation in chronic myeloid leukemia cells
... detect DNA damage caused by DSBs, SSBs, alkali labile sites, oxidative base damage, and DNA cross-linking with DNA or proteins ...[27]. Repair capacities of all above lesions were compared by ...
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A Genomics-Based Screen for Yeast Mutants With an Altered Recombination/End-Joining Repair Ratio
... break repair (DSBR; re- only when the termini are incompatible and require pro- viewed in Paques and Haber 1999; Jackson ...1999). Repair by homologous recombination involves the con- Artemis and DNA ...
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Multiple Barriers to Nonhomologous DNA End Joining During Meiosis in Drosophila
... noncrossover repair product while also cre- ating an equal, yet low likelihood of crossover formation along the entirety of chromosome ...regulate DNA synthesis following resection and strand invasion ...
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Overcoming obstacles to nonhomologous end joining repair of chromosome double strand breaks
... Similarly, repair of UV lesions in yeast occurs less efficiently when the lesion occurs in the context of heterochromatic telomere regions as opposed to euchromatin ...impedes DNA repair. In the case ...
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The proteomic investigation reveals interaction of mdig protein with the machinery of DNA double-strand break repair
... integrity, DNA replication, gene transcription, signal transduction, and immune ...non-homologous end-joining (NHEJ) DNA repair and chromatin binding, including Ku80 (XRCC5), Ku70 ...
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Imprecision and DNA break repair biased towards incompatible end joining in leukemia
... template DNA from two independent experiments were pooled and sent for sequencing on the miseq platform ...input DNA from cells descending from four independent experiments was titrated from 25ng to ...Taq ...
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Impaired 53BP1/RIF1 DSB mediated end-protection stimulates CtIP-dependent end resection and switches the repair to PARP1- dependent end joining in G1
... 5’ end resection is activated to produce DSBs with 3’ single stranded overhangs to commit the repair to HR [8, ...DSB end resection ...initiate end resection and commit the repair to HR ...
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Differential Suppression of DNA Repair Deficiencies of Yeast rad50, mre11 and xrs2 Mutants by EXO1 and TLC1 (the RNA Component of Telomerase)
... the end-joining path- way of DSB repair was examined by performing plasmid end-joining assays (see materials and ...pRS314 DNA yielded transformants as efficiently as supercoiled ...
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Unique molecular mechanisms for maintenance and alteration of genetic information in the budding yeast Saccharomyces cerevisiae
... DSB repair and cell survival after DNA damage is only apparent when HR is unavailable in ...foreign DNA with homology to target genes, the ideal substrates for HR, are ...microhomology-mediated ...
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