DNA fragmentation
Association of Sperm Aneuploidy Frequency and DNA Fragmentation Index in Infertile Men
... unrepaired DNA breaks were generated as a result of defective spermiogenesis, and oxidative stress could be the other mechanism for DNA damage ...sperm DNA fragmentation in infertile men is ...
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HIGH LEVELS OF DNA FRAGMENTATION OBSERVED IN NORMOSPERMIC MEN DUE TO EXPOSURE TO PESTICIDES
... fragmented DNA (SCSA–%DFI) there by increasing the weight of evidence that exposure to high levels of air pollution may have damaging effects on sperm ...of DNA fragmentation spermatozida the second ...
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Sperm DNA fragmentation, recurrent implantation failure and recurrent miscarriage
... 5 min. Each slide was then dipped into a beaker of 1 × TBS 2–3 times and excess liquid gently tapped off and then the smear covered with 100 µl of 1 × TdT equilibration buffer supplied by the kit and incubated at room ...
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Presence of DNA Fragmentation and Lack of Neuroprotective Effect in DFF45 Knockout Mice Subjected to Traumatic Brain Injury
... genomic DNA in neu- rons from injured cortex and hippocampus after ...TBI-induced DNA fragmentation and associated neuronal apoptosis in mice, by comparing effects in DFF45 knockout mice and ...
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Spermatozoal cell death-inducing DNA fragmentation factor-α-like effector A (CIDEA) gene expression and DNA fragmentation in infertile men with metabolic syndrome and normal seminogram
... both DNA fragmentation and CIDEA gene expression could be due to the same causes and happen in the same circumstances, a positive correlation between them appears reasonable, and both of them could be ...
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The relationship between sperm viability and DNA fragmentation rates
... sperm DNA fragmentation and poor sperm viability are linked to male ...between DNA fragmentation and sperm via- bility ...in DNA fragmentation, either by an active mechanism ...
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Increased DNA Fragmentation in Patients with Infertility in Dakar (Senegal)
... of DNA fragmentation (p < ...15% DNA fragmentation separating the control group from the group of patients with infertility but this his data was obtained with an area under the curve at ...
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Prolactin Supplementation in Sperm Preparation A Study of Sperm DNA Fragmentation
... the DNA integrity of the ...sperm DNA Fragmentation index ...sperm DNA fragmentation in sperm preparation, particularly in moderate and severe DFI ...
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Sperm DNA fragmentation after radioiodine treatment for differentiated thyroid cancer
... and DNA although it is known that treatment with 131 I induces radiation to the testes [4], mainly through contamination of blood and bladder ...cell DNA, so it makes sense to assume that radiation is- sued ...
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Sperm DNA Fragmentation and Chromatin Maturation of Prepared Sperm with ALA Supplementation: The Effect of Embryo Development Quality
... ALA supplementation in sperm preparation could enhance the embryo quality. Previous research showed that ROS induced the oxidative stress if the amount exceeds capacity of antioxidants in the cells, including spermatozoa ...
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Varicocele and DNA Fragmentation in Senegalese Infertile Males—A Study of 95 Cases
... Sperm DNA Fragmentation TUNEL test (Terminal deoxynucleotidyl transferase dUTP nick end labeling) was performed to assess sperm DNA fragmentation as described by Sharma’s [19] ...with ...
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DNA Fragmentation Pattern and its Application in DNA Sample Type Classification
... of DNA fragments gives important information for the construction of physical genome maps and ...the DNA fragment length and the DNA molecule ...single DNA molecules or of DNA-protein ...
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Sperm DNA Fragmentation in Petroleum Workers
... normal DNA which represented by the big and medium halos as seen in figure (6,7) and the sperm with damaged DNA represented by the small or without halo in addition to degraded ones figure ...sperm ...
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Sperm DNA fragmentation and oxidation are independent of malondialdheyde
... Flow cytometric analysis was carried out using an EPICS XL flow cytometer (Beckman Coulter) equipped with a 15mW argon-ion laser for excitation at 488 nm. At least 10000 events per sample were analysed. Light-scattering ...
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Single sperm selection and DNA fragmentation analysis: The case of MSOME/IMSI
... sperm DNA damage in selected single spermatozoa, therefore, corroborate the existence of a direct relationship between sperm morphological patterns and DNA ...
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Fragmentation of DNA affects the accuracy of the DNA quantitation by the commonly used methods
... of DNA (detection of less than 1 genomic equivalent is im- portant in non-invasive prenatal diagnostics [21]), the detection of (q)PCR inhibitors in the analysed sample (important for forensic DNA analyses ...
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Antimalarial drug artemether inhibits neuroinflammation in BV2 microglia through Nrf2 dependent mechanisms
... the DNA was investigated using EMSA. MTT, DNA fragmentation and ROS assays in BV2-HT22 neuronal co-culture were used to evaluate the effects of artemether on neuroinflammation-induced neuronal ...
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The influence of leukocytospermia on the outcomes of assisted reproductive technology
... Male infertility represents an important etiological factor for infertile couples to consider, and semen analysis is the main screening tool that guides diagnosis. Among the parameters evaluated in the seminal analyses, ...
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Evaluation the Efficacy of HBA Sperm- Hyaluronan Binding Assay Method in Infertility Centers
... Selection of spermatozoa based on their electronega- tive surface charge, apoptotic markers, membrane maturity (HB binding), and ultramorphology has been used to develop technical protocols for sperm isolation. The tests ...
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The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent
... DNA fragmentation of cells treated with GPS was assessed quantitatively using DNA end-labelling (TUNEL) and flow cytometry (Figure ...and DNA nicks were identified with an anti-BrdU monoclonal ...
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