DNA gyrase
ESTABLISHED PHYTOLIGANDS FROM TRIDAX PROCUMBENS LINN AGAINST BACTERIAL DNA GYRASE B RECEPTOR: MOLECULAR DOCKING APPROACH
... www.wjpr.net Vol 7, Issue 12, 2018. 588 In the present results, among other established phytochemicals of T. procumbens, Epigallocatechin-3-gallate showed highest binding energy (-7.8 Kcal/mol), followed by binding ...
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In Vitro Inhibitory Effect of Methanol Extracts of Chinese Herbal Drugs on Supercoiling Activity of Bacterial DNA Gyrase
... Bacterial DNA gyrase is a proven target for antibacterial ...on DNA gyrase was tested using gel-based DNA supercoiling ...of DNA gyrase, and the others were either less ...
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PprA Protein Is Involved in Chromosome Segregation via Its Physical and Functional Interaction with DNA Gyrase in Irradiated Deinococcus radiodurans Bacteria
... of DNA gyrase and, thus, chose to focus our work on characterizing the activities of the deinococcal DNA gyrase in the presence or absence of ...deinococcal DNA gyrase, like ...
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Clinically Relevant Mutant DNA Gyrase Alters Supercoiling, Changes the Transcriptome, and Confers Multidrug Resistance
... chromosomal DNA exists in a complicated, con- densed state in which the nucleoid consists of a large number of domains of independently supercoiled DNA ...chromosomal DNA is not fixed, and the ...
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Design, synthesis and antibacterial properties of pyrimido[4,5-b]indol-8-amine inhibitors of DNA gyrase
... their DNA, resulting in eventual cellular ...the DNA binding site of topoisomerases in order to elicit their therapeutic effect, however, recent publications have focused on inhibition at an alternative ...
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TOWARDS THE TOTAL SYNTHESIS OF PHORBOXAZOLE A, SYNTHESIS AND BIOLOGICAL EVALUATION OF COUMARIN ANALOGUES AS DNA GYRASE INHIBITORS AND DEVELOPMENT OF NEW SYNTHETIC METHODOLOGIES
... A hybrid molecule synthesis strategy was employed to develop new molecules with potential antibacterial activity. Three types of coumarin-linked hybrids have been synthesized. The coumarin ring system has been linked to ...
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Detection of DNA Gyrase Mutation and Multidrug Efflux Pumps Hyperactivity in Ciprofloxacin Resistant Clinical Isolates of Pseudomonas aeruginosa
... highly hydrophobic Isoleucine may affect the gy- rase-quinolone interaction by loss of necessary en- zyme-drug contacts or conformational changes that may eventually result in antibiotic resistance. The presence of ...
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Alternations in DNA gyrase genes in low-level fluoroquinolone-resistant <em>Moraxella catarrhalis</em> strains isolated in Poland
... inhibit DNA synthesis by targeting two essential topoisomerases in bacterial ...one, DNA gyrase, an enzyme found exclusively in bacteria, uses the energy of ATP hydrolysis to introduce negative ...
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Pyridine-3-carboxamide-6-yl-ureas as novel inhibitors of bacterial DNA gyrase: Structure based design, synthesis, SAR and antimicrobial activity
... of DNA gyrase and topoisomerase IV possessing potent antibacterial activity: Intelligent design and evolution through the judicious use of structure-guided design and stucture- activity relationships, ...
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Identification of Mycobacterial Species by PCR Sequencing of Quinolone Resistance Determining Regions of DNA Gyrase Genes
... The determination of the amino acid sequence of quinolone resistance-determining regions (QRDRs) in the A and B subunits of DNA gyrase is the molecular test for the detection of fluoroquinolone resistance ...
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BACTERIAL DNA GYRASE IS NOT THE TARGET OF QUINOLINE-BASED ANTI-TUBERCULOSIS COMPOUNDS
... of DNA gyrase was very clearly shown in Figure 2B. The relaxed DNA (lane1) was found to be completely converted into supercoiled DNA in the presence of DNA gyrase (lane ...of ...
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MOLECULAR DOCKING STUDIES OF 4-BENZYLIDENE-2-(4-HYDROXY-3-METHOXYSTYRYL) OXAZOL-5(4H)-ONES; INTERACTIONS WITH ARG-136 AND ASP-73 OF DNA GYRASE
... binding pocket Arg-136 and Gly-164 (1-3). Aminocoumarins such as Novobiocin, cyclothialidines and fluoroquinolones like ciprofloxacin are conventional DNA gyrase inhibitors (3). Heterocyclic compounds ...
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Evaluation of Antimicrobial Profile of Some Novel 1, 3, 4-Oxadiazole Derivatives Followed by Molecular Docking Against 3G7E Bacterial DNA Gyrase
... Here we concluded that the compound AB2, AB1, AB7, AB3, AB4 and AB8 possessed potential antimicrobial activity against following bacteria: S. aureus (ATCC 9144), B. subtilis (ATCC 6633), S. epidermidis (ATCC 12228), P. ...
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Involvement of host DNA gyrase in growth of bacteriophage T5.
... Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest When nalidixic acid was present during determinations of average burst size in these temperature-sensitive strains, it w[r] ...
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Molecular Cloning of Apicoplast-Targeted Plasmodium falciparum DNA Gyrase Genes: Unique Intrinsic ATPase Activity and ATP-Independent Dimerization of PfGyrB Subunit
... of DNA or a GyrA subunit, we performed an ATPase assay using either wild-type or different deletion mutants of ...of gyrase B is confined to the N terminus domain that is included in the deletion mutant ...
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Involvement of DNA gyrase in replication and transcription of bacteriophage T7 DNA.
... This is particularly true of RNA synthesis, where only late transcription appears to be inhibited by coumermycin; but with DNA synthesis, which is more completely inhibited by the antibi[r] ...
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DNA gyrase inhibitors block development of Bacillus subtilis bacteriophage SP01.
... The addition of increasing quantities of either nalidixic acid or novobiocin caused a progressive delay of the lysis, which took place at 390 min with 10 ,ug of novobiocin 855 Downloaded[r] ...
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Insights into Pyrazinamidase and DNA Gyrase Protein Structures in Resistant and Susceptible Clinical Isolates of Mycobacterium tuberculosis
... activity. Gyrase is a multi-subunit ...of DNA at the active site is amino acid tyrosine ...block DNA replication and lead to cell death (7, ...
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A Superhelical Spiral in the Escherichia coli DNA Gyrase a C-terminal Domain Imparts Unidirectional Supercoiling Bias
... for gyrase function and will then return to the issue of Topo IV ...coli gyrase results in loss of the bias toward introduc- tion of ( ⫺ ) supercoils but retention of core Topo II activity ...CTD-less ...
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High Proportion of Fluoroquinolone Resistant Mycobacterium tuberculosis Isolates with Novel Gyrase Polymorphisms and a gyrA Region Associated with Fluoroquinolone Susceptibility
... in DNA, by binding to DNA gyrase. DNA gyrase consists of two A and two B subunits encoded by the gyrase A (gyrA) and gyrase B (gyrB) genes, ...and DNA gyrase ...
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