DNA hybridization
Measuring DNA hybridization using fluorescent DNA-stabilized silver clusters to investigate mismatch effects on therapeutic oligonucleotides
... detecting DNA–DNA binding to analyze the nucleotide-mismatch dependency on DNA ...of hybridization to the target sequence is determined by measuring the fluorescence ...
8
Use of Multiple Displacement Amplification and Checkerboard DNA DNA Hybridization To Examine the Microbiota of Endodontic Infections
... a DNA ampli- fication ...of DNA targets (3, 11, 33, 63, 64), increasing the amount of DNA obtained from the endodontic bacterial ...amplified DNA to perform multiple analyses of the same ...
11
Optimization of a horizontal slot waveguide biosensor to detect DNA hybridization
... the DNA-hybridization process where the ssDNA is base-paired with another ssDNA to form double- stranded DNA (dsDNA) is studied by using a horizontal ...as DNA. Change in the effective index ...
9
Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison
... The pragmatic species concept for Bacteria and Archaea is ultimately based on DNA-DNA hybridization (DDH). While enabling the taxonomist, in principle, to obtain an estimate of the overall similarity ...
18
Chemiluminescent Detection of DNA Hybridization Based on Signal DNA Probe Modified with Gold and Cobalt Nanoparticles
... of DNA hybridization, based on electrochemical stripping detection of the colloidal gold ...of DNA targets using CL with a detection limit of 5 fM ...of DNA hybridization based on gold ...
12
Use of the checkerboard DNA DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L )
... the hybridization of the phos- phatase alkaline-labeled bacterial probes with defined amounts of total genomic DNA extracted from each bac- terial species are shown in Figure ...with DNA amounts ...
5
Synthesis and Characterization of a Dye Sensitizing Molecule for Photoelectrochemical DNA Hybridization Detection
... incorporating DNA until 1997 2 . DNA biosensors, which utilize DNA hybridization techniques, offer an advantage over many other biosensors in that DNA is very stable and can be readily ...
105
Comparison of DNA Amplification, mRNA Amplification, and DNA Hybridization Techniques for Detection of Cytomegalovirus in Bone Marrow Transplant Recipients
... assay, DNA hybridization is followed by signal amplification and chemiluminescence ...CMV DNA test panel as ...571,514 DNA copies per ml); this was altered to ...571,514 DNA copies per ...
8
Bismuth Oxide Nanoparticles/Chitosan/Modified Electrode as Biosensor for DNA Hybridization
... of DNA hybridization usually involves monitoring of a current response, resulting from the Watson–Crick base-pair recognition event, under controlled potential conditions ...target DNA whose ...
12
Detection of Corynebacterium kutscheri in animal tissues by DNA DNA hybridization
... kutscheri were isolated, radiolabeled with 32p, and used in DNA-DNA hybridization assays with blots of impression smears of animal tissues on membrane filters.. kutscheri probes did not [r] ...
5
DNA HYBRIDIZATION IN THE GENUS DROSOPHILA
... The nucleotide similarity relationships using the slow annealing DNA of five species were obtained and compared with the fast annealing DNA relationships.. The species relations[r] ...
14
An Electrochemical DNA-Hybridization Assay for Acinetobacter baumannii Detection
... the hybridization of DNA, 3 strategies were used in the present study, and their performances were ...target DNA has a better chance of attaining the best orientation for ...solution ...
11
DNA hybridization probe for clinical diagnosis of Entamoeba histolytica
... In contrast, a second class of recombinant plasmid clones pEH3, pEH4, pEH6, and pEH10 recognized repeated DNA sequences which were conserved across Entamoeba species Fig.. These nonspeci[r] ...
6
Blue shift of CdSe/ZnS nanocrystal-labels upon DNA-hybridization
... embedded Arabidopsis thaliana inflorescence were de- waxed by placing them in 3 changes of histoclear (PLANO GmbH) for 3 minutes each, followed by 2 changes of histoclear/ethanol (2:1 and then 1:2) followed by 3 changes ...
6
COMPARISON OF THE DNA OF SIX VICIA SPECIES BY THE METHOD OF DNA-DNA HYBRIDIZATION
... If the difference in DNA content per cell between two species were due to uneven multipli- cation of DNA messages (local multiplicity), more labeled DNA would he hybridi[r] ...
18
Characterization of the new Chlamydia agent, TWAR, as a unique organism by restriction endonuclease analysis and DNA DNA hybridization
... Moreover, restriction patterns that distinguished TWAR from the other Chlamydia species were observed with all other enzymes tested data not shown.. Of the Chlamydia isolates that have b[r] ...
6
Identification of Borrelia burgdorferi and B hermsii using DNA hybridization probes
... Probe 3G, a 445-bp Pstl-HitidIII fragment, hybridized strongly with plasmid-enriched DNA and with whole spirochetes oftwo North American strains of B.. blrgdoefèri B31 and HB19 but not w[r] ...
5
Development of a diagnostic test for Johne's disease using a DNA hybridization probe
... par-atithebri-culosis genomic DNA identified as M13 mpHAW71, was used to test fecal samples submitted to the Central Animal Health Laboratory for mycobacterial culturing.. parautlberc-ul[r] ...
6
Characterization of Vibrio metschnikovii and Vibrio gazogenes by DNA DNA hybridization and phenotype
... gazogenes strains were tested at 25°C on Mueller-Hinton agar with 1.5% NaCl added, because they did not grow without the additional NaCl.. Sizes of zones of inhibition are given for V.[r] ...
8
Identification of Mycobacterium kansasii by DNA hybridization
... Briefly, this procedure involves digestion of chromosomal DNA with restriction enzymes and Southern blot hybridization by using plasmid pKK3535 as the probe.. This plasmid contains the r[r] ...
5