DNA polymerase-chain-reaction
A COMPARATIVE STUDY BETWEEN A NUMERICAL AND RAPD-PCR METHODS FOR THE IDENTIFICATION OF SOME STREPTOMYCETE STRAINS
... polymorphic DNA-polymerase chain reaction (RAPD-PCR) technology for identification of 19 streptomycetes belonging to different series color (gray, 4 strains; red, 8 strains divided into three ...
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Polymerase chain reaction on a viral nanoparticle
... a DNA detection system where the presence of target DNA was detected by the formation of an extended amplimer on the surface of the M13 which would lead to an alteration in the LD ...the DNA primers ...
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Clustering techniques for DNA computing readout method based on real-time polymerase chain reaction
... of DNA molecules, which are negatively charged [9]. DNA molecules move towards the positive electrode at different speed in the electric ...of DNA mixture in a gel depends heavily on the gel porosity ...
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Detection of Enhanced Green Fluorescent Protein DNA in Pink Bollworm through Polymerase Chain Reaction
... stained DNA fragments amplified by diagnostic ...template DNA from enhanced green fluorescent protein-positive insects and are ...contains DNA size standard (Gibco-BRL catalog ...Template DNA ...
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DETECTION OF MULTIPLE MICROBIAL DNA IN ATHEROMATOUS PLAQUES BY POLYMERASE CHAIN REACTION
... A thorough clinical examination was conducted for all the patients and detailed history regarding the presence of various risk factors such as hypertension, hyperlipidemia, diabetes mellitus and smoking were recorded. ...
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Polymerase chain reaction assay of parvovirus B19 DNA in clinical specimens
... In a control panel of 100 serum samples from individuals with other infections, only 1 serum sample was found positive by PCR, and this was also found positive by dot hybridization.. Thi[r] ...
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Detection of Trypanosoma cruzi by DNA amplification using the polymerase chain reaction
... Photograph of an agarose gel stained with ethidium bromide containing the amplification products of DNAs obtained from the urine or abdominal contents of four reduviid bugs, designated 1[r] ...
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Detection of human parvovirus B19 DNA by using the polymerase chain reaction
... In this study, 20 serum samples were positive after ethidium bromide staining, and only 4 serum samples required a radiolabeled probe for detection of amplified B19 DNA.. Third, an autom[r] ...
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Polymerase chain reaction based detection of Trypanosoma cruzi DNA in serum
... Lanes 1 to 3, samples from baby I on the day of birth and 1 and 3 months later, respectively; lanes 5 and 6, samples from baby II on the day of birth and 5 months later; lane 8, sample f[r] ...
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Detection of Coxiella burnetti by DNA amplification using polymerase chain reaction
... burnetii isolates by PCR in tissue culture material and in direct patient specimens heart valves using oligonucleotide primers derived from the superoxide dismutase gene sequence of C.. [r] ...
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Detection of enterotoxigenic Escherichia coli after polymerase chain reaction amplification with a thermostable DNA polymerase
... The direct identification of enterotoxigenic Escherichia coli from clinical specimens was examined by using the polymerase chain reaction PCR for amplifying the heat-labile toxin LT gene[r] ...
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Development of methodical approach to the identification of the features of the genetic polymorphisms and gene expression in children under influence of chemical environmental factors on the example of strontium
... of polymerase chain reaction, DNA sequenc- ing fragments, studies of spontaneous and induced strontium expression of candidate genes that help identify changes in the genome and transcriptome ...
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Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis
... Sequence Analysis and Amplification by Polymerase Chain Reaction of a Cloned DNA Fragment for Identification of Mycobacterium tuberculosis RUBINA J.. WIRTH2* American Registry of Patholo[r] ...
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Application of PCR to multiple specimen types for diagnosis of cytomegalovirus infection: comparison with cell culture and shell vial assay
... Development and clinical significance of a diagnostic assay based on the polymerase chain reaction for detection of human cytomegalovirus DNA in blood samples from immunocompromised pati[r] ...
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DNA analysis by polymerase chain reaction of saliva traces on cigarette butts exposed to indoor and outdoor environmental conditions
... Cigarette butts are one of the most common carriers of saliva traces in forensic practice. Today, approximately 1.2 billion people worldwide smoke tobacco (Oleski et al., 2010) and many people smoke when they are nervous ...
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Development of real time polymerase chain reaction for detection of Salmonella typhimurium and Salmonella enteritidis in fish
... The conv enti onal m ethod of Salmonella identification in food uses 25 g of food product with 225 ml of diluents, to make a final volume of 250 ml (a 1/10 dilution). That 25 g of sample is used to increase the ...
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Direct method for detecting small quantities of hepatitis B virus DNA in serum and plasma using the polymerase chain reaction
... inhibit DNA polymerase, thereby obviating direct detection of serum viral DNA sequences by the polymerase chain reaction ...
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Amplification of bacterial 16S ribosomal DNA with polymerase chain reaction
... By directing oligonucleotide primers at sequences conserved throughout the eubacterial kingdom, we amplified bacterial 16S ribosomal DNA sequences with the polymerase chain reaction.. Pr[r] ...
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Amplification of a species specific DNA fragment of Mycobacterium tuberculosis and its possible use in diagnosis
... Sequence analysis and amplification by polymerase chain reaction of a cloned DNA fragment for identification of Mycobacterium tuberculosis.. de la Maza.[r] ...
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Construction of a DNA probe and detection of Actinobacillus pleuropneumoniae by using polymerase chain reaction
... From the nucleotide sequence of the putative genomic probe, three primers were synthesized for use in polymerase chain reactions PCRs, with 31 strains tested by using purified and crude [r] ...
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