DNA primers for PCR
Specific detection of Pythium aphanidermatum from hydroponic nutrient solution by booster PCR with DNA primers developed from mitochondrial DNA
... aphanidermatum was detected by a hybridization assay of total DNA extracted directly from infected roots.. deliense are morphologically similar, with smooth oogonia, aplerotic o[r] ...
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PCR-Based Diagnosis of Neoscytalidium dimidiatum Infection Using Internal Transcribed Spacer 1 Region of Ribosomal DNA Primers
... study, PCR identification with N. dimidiatum-specific primers based on ITS1 region required approximately 3 hours to provide accurate identification in patients with superficial skin and nail ...
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PCR primers Universal primers for PCR am-
... PCR products of a mixture of RB and 52 templates were transcribed and the RNA was analysed in gel (Figure 3). The intensities of the RNA bands of each virus isolate were 2–2.5 times higher than those of the bands ...
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Development of a Universal Intimin Antiserum and PCR Primers
... chromosomal DNA, purified from 1-ml overnight bacterial cultures with the QIAamp tissue kit (QIAGEN), was used as a ...template. PCR was performed with the Gene Amp XL PCR kit (Perkin-Elmer) and ...
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Use of PGMY Primers in L1 Consensus PCR Improves Detection of Human Papillomavirus DNA in Genital Samples
... first PCR run, al- though our negative controls always scored negative, or vari- able amplification of very small amounts of target ...consensus PCR assay and not by the PGMY-line ...PGMY primers or ...
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Impact of Enzymes and Primers on the PCR Amplification of Some Goatfishes
... of PCR amplification of target genes from genomic DNA (gDNA) of many environmental specimens (Schneider, Enkerli, & Widmer, ...of PCR, but normally inhibit the polymerase from amplifying the ...
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A database of PCR primers for the chloroplast genomes of higher plants
... An overall scheme on the construction of the database is given in Figure 1. Published articles were screened in a random fashion for new primer information between 1999 and 2005. This included scanning the tables of con- ...
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PRISE2: software for designing sequence-selective PCR primers and probes.
... One distinctive feature of PRISE2 is that it allows users to emphasize primer selectivity at the 3’end. This feature is essential for selective amplification of conserved se- quences such as rRNA genes, and to accurately ...
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Detection of Mycobacterium ulcerans by real-time PCR with improved primers
... DNA extraction and purification Bacteria were collected by centrifugation at 16,200×g for 2 min and resuspended in a solution containing 20 μl of 0.5 M NaOH, 4 μl of 10 % sodium dodecyl sulfate, and 180 μl of ...
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A systematic guideline for developing the best real-time PCR primers
... the primers must detect every alternative transcript and splicing variant of the queried ...of PCR cycling ...with DNA from the organism used to over- express and purify the ...the primers ...
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Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
... universal PCR simultaneously amplifies DNA from food items present in DNA purified from stomach samples, while the predator's own DNA is blocked from amplification by the addition of a ...
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Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs
... universal PCR simultaneously amplifies DNA from food items present in DNA purified from stomach samples, while the predator's own DNA is blocked from amplification by the addition of a ...
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Design of an improved set of oligonucleotide primers for genotyping MeCP2tm1.1BirdKO mice by PCR
... a PCR product with either one pair of primer or the other (P5-P6 for the KO, P5-P7 for the ...of PCR reactions, with the unexpected band being slightly more diffuse and higher than the other ...yields ...
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Specific Primers for Rapid Detection of Microsporum audouinii by PCR in Clinical Samples
... MA1 primers. This shows that MA1 primers had 100% accuracy in the study ...future, PCR-based identification, as outlined in this study, can be applied directly to skin and hair ...The PCR ...
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PCR & DNA Sequencing. PCR= Polymerase Chain Reaction. PCR applications
... "evolutionary PCR", one may increase the chances of getting product by designing "degenerate" primers: Degenerate primers= a set of primers which have a number of options at several positions ...
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Amplification of human papillomavirus DNA sequences by using conserved primers
... Use of primers derived from sequences shared by different virus types for PCR amplification of papillomavirus DNA se- quences could be of value for the identification of viral.. sequence[r] ...
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Evaluation of PCR Primers for Early Diagnosis of Cytomegalovirus Infection following Liver Transplantation
... CMV DNA in PBLs and serum by PCR had high levels of sensitivity and specificity for the detection of CMV infection and a high degree of sensitivity but a lower degree of specificity for the detection of ...
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Characterization and Identification of F1 Hybrids in Eucalyptus by PCR Study using Random Primers
... Key words: Eucalyptus, hybrids, Polymerase chain reaction, Random Amplified Polymorphic DNA. The genus Eucalyptus L. Herit. belongs to the family Myrtaceae and comprises about 700 species. These species show a lot ...
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Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers
... the PCR sequences of several amplified frag- ments of the ...HBV DNA structure causes the instability of exposed HBV DNA and the low efficiency of whole genome ...of primers for full length ...
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An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers
... nuclear DNA was demonstrated by Delseny et ...ideal DNA mark- ers for genetic mapping and population-genetic studies because of their abundance, co-dominant character, ease of detection by PCR, ...
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