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DNA unwinding

Common determinants in DNA melting and helicase-catalysed DNA unwinding by papillomavirus replication protein E1

Common determinants in DNA melting and helicase-catalysed DNA unwinding by papillomavirus replication protein E1

... processive DNA unwinding (helicase activity) by ...unwind DNA, even the equivalent Ala substitution that is inactive in T-antigen ...a DNA binding site that is intolerant even to subtle ...

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DNA Unwinding Functions of Minute Virus of Mice NS1 Protein Are Modulated Specifically by the Lambda Isoform of Protein Kinase C

DNA Unwinding Functions of Minute Virus of Mice NS1 Protein Are Modulated Specifically by the Lambda Isoform of Protein Kinase C

... NS1 DNA-unwinding ...of DNA-unwinding activity, the S473A mutant was severely impaired for DNA replication, as well as site- and strand-specific nicking of double-stranded left-end ...

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Two Regions of Simian Virus 40 T Antigen Determine Cooperativity of Double-Hexamer Assembly on the Viral Origin of DNA Replication and Promote Hexamer Interactions during Bidirectional Origin DNA Unwinding

Two Regions of Simian Virus 40 T Antigen Determine Cooperativity of Double-Hexamer Assembly on the Viral Origin of DNA Replication and Promote Hexamer Interactions during Bidirectional Origin DNA Unwinding

... viral DNA rep- ...viral-origin DNA, and locally distorts the origin DNA structure, but it cannot catalyze origin DNA ...the DNA binding domain (class 4) has remarkably similar ...

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Cryo EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation

Cryo EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation

... Thus, mechanistic insight informing on the sequence of events taking place in the donor cell during conjugation emerges from the results presented here and is recapitulated in the scheme pre- sented in Figure S7. All ...

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Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein).

Mutator mutations in bacteriophage T4 gene 32 (DNA unwinding protein).

... Since previous reports 8, 9, 17 already indicate that mutational lesions in gene 32 affect mutation rates, we have systematically surveyed the effects of such mutations upon the rates of[r] ...

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Cryo EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation

Cryo EM structure of a relaxase reveals the molecular basis of DNA unwinding during bacterial conjugation

... stranded DNA (ssDNA), and translocates it into a recipient cell via an extracellular pilus (Costa et ...a DNA-processing step medi- ated by another multi-protein complex, termed ‘‘the relaxo- some’’ ...

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Study of SV40 large T antigen nucleotide specificity for DNA unwinding

Study of SV40 large T antigen nucleotide specificity for DNA unwinding

... In addition to cW393 and cL557, the unique architec- ture of multiple hydrophobic lining in the NTP binding pocket of LT consists of six other leucines (cL394, cL397, cL398, cL553, cL563, cL564) and one isoleucine ...

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Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures

Human RECQ1 helicase-driven DNA unwinding, annealing, and branch migration: insights from DNA complex structures

... the DNA annealing activity, which is not detected when the hairpin is present (Table ...impair DNA helicase activity but have little impact on the annealing activity, suggesting that other structural ...

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Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication

Analyses of the Interaction between the Origin Binding Domain from Simian Virus 40 T Antigen and Single-Stranded DNA Provide Insights into DNA Unwinding and Initiation of DNA Replication

... duplex DNA enters the central channel, and then at least one strand transits to the helicase surface via the side channels (23, 29, 44, ...for DNA replication, may serve the analogous function of enabling ...

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Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication

Role of the Hydrophilic Channels of Simian Virus 40 T-Antigen Helicase in DNA Replication

... The third residue in this class (D429) may participate in linking the energy from ATP hydrolysis to DNA unwinding. That residue is adjacent to I428, which moves in different nucleotide states and could ...

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GKE122.pdf

GKE122.pdf

... for DNA unwinding by helicases has been described in terms of an ‘active’ or ‘passive’ mechanism ...duplex unwinding via a passive ...and unwinding of double-stranded ...stranded DNA ...

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Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro

Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro

... SV40 DNA replication in ...SV40 DNA replication in vitro, while read- dition of TBP complexes to the extracts reduced their replica- tion ...bidirectional unwinding of the viral origin ...SV40 ...

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11516.full.pdf

11516.full.pdf

... optimal DNA unwinding ...processive DNA unwinding is observed only when the enzyme concentra- tion greatly exceeds the substrate ...The DNA unwinding results in ...

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DNA induces conformational changes in a recombinant human minichromosome maintenance complex

DNA induces conformational changes in a recombinant human minichromosome maintenance complex

... ATP-dependent DNA unwinding activity has been demon- strated for recombinant archaeal homohexameric minichromo- some maintenance (MCM) complexes and their yeast hetero- hexameric counterparts, but in higher ...

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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.

Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.

... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ...

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Initiation of Minute Virus of Mice DNA Replication Is Regulated at the Level of Origin Unwinding by Atypical Protein Kinase C Phosphorylation of NS1

Initiation of Minute Virus of Mice DNA Replication Is Regulated at the Level of Origin Unwinding by Atypical Protein Kinase C Phosphorylation of NS1

... specific DNA binding (15); thus, we were not able to perform the nicking reactions in absence of ...for DNA unwinding (36), these results demonstrate that the ssNick origin can be cleaved in the ab- ...

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Bacteriophage T4-Directed DNA Synthesis in Toluene-Treated Cells

Bacteriophage T4-Directed DNA Synthesis in Toluene-Treated Cells

... Temperature-sensitive mutants in Some synthesis at early times after infection genes 32 which codes for a DNA-unwinding was observed in our in vitro system with an protein [1], 41, and 4[r] ...

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JBC-2009-Jennings-4806-14.pdf

JBC-2009-Jennings-4806-14.pdf

... Excess DNA in Vitro—The data in the single turnover unwinding experiments suggested that an oligomeric species of NS3 was responsible for optimal DNA unwinding ...turnover DNA ...

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AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.

AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.

... T-antigen-mediated unwinding of DNA con- taining the origin of DNA replication (16, 43, ...46). Unwinding of origin DNA in vitro is dependent upon the concentration of T antigen (57) ...

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The effect of different methods and image analyzers on the results of the in vivo comet assay

The effect of different methods and image analyzers on the results of the in vivo comet assay

... and DNA unwinding and electrophoresis times had a large impact on the equivalence of the results between the different methods used for the in vivo comet ...and DNA unwinding and ...

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