DNA vectors and plasmids
Comparative analysis of DNA vectors at mediating RNAi in Anopheles mosquito cells and larvae
... Transient dsRNA-mediated gene silencing in A. stephensi larvae To examine whether the RNAi-mediated gene silencing observed in A. gambiae cells could also be achieved in vivo, the activity of the dsRNA constructs was ...
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Sindbis virus DNA-based expression vectors: utility for in vitro and in vivo gene transfer.
... Several DNA-based Sindbis virus vectors were constructed to investigate the feasibility and potential applications for initiating the virus life cycle in cells transfected directly with plasmid ...These ...
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The development and application of methods for using 'Agrobacterium' spp as DNA vectors in soft fruit plants
... of DNA transfer using Agrobacterium is that the high level of phytohormones produced in transformed tissue, due to expression of genes on the T-DNA, prevents cell regeneration into whole ...
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Neutralizing Antibodies Elicited by Immunization of Monkeys with DNA Plasmids and Recombinant Adenoviral Vectors Expressing Human Immunodeficiency Virus Type 1 Proteins
... the DNA priming-rAd5 boost- ing immunization platform can elicit robust HIV-1-specific an- ti-Env antibody responses in nonhuman ...after DNA- rAd5 immunization of monkeys, but these have focused on ...
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Vectors for gene therapy: A place for DNA transposon
... Figure 2. Schematic view of a DNA-transposon system for gene delivery. Both donor and helper plasmids are transfected into the host cells. The helper plasmid is an expression vector that allows transposase ...
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Comparison of nanoparticle-mediated transfection methods for DNA expression plasmids: efficiency and cytotoxicity
... pIRES-hrGFPII plasmids used ...used vectors differ in that, apart from the GFP, the HMGB1 vector encodes a single chain protein, while the IL-12 vector encodes a complex protein consisting of two dif- ...
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Integration of DNA into bacterial chromosomes from plasmids without a counter selection marker
... linear DNA (3). We have con- structed all the plasmids necessary to undertake such multi-step ACE strategies at each of the loci studied here (Table ...lambda DNA was inserted into the chromosome of ...
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Analyses of circular DNA molecules in the leafhopper Psammotettix alienus for identification of viruses and plasmids
... ii ABSTRACT Microorganisms often depend on vectors for transmission. A single vector may carry different species of microorganisms such as fungi, bacteria and viruses. In Sweden, leafhoppers of the species ...
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The roles of adenoviral vectors and donor DNA structures on genome editing
... donor DNA template, the percentage of stably transduced cells was lower that obtained following the use of IDLV donor DNA ...donor DNA templates were found to be properly inserted at the target site, ...
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Development of new plasmid DNA vaccine vectors with R1-based replicons
... phase of the seed can significantly impact the productivity of resulting cultures when using runaway replication plas- mids for recombinant protein production [14]. To test the sensitivity of DH5α[pDMB02-GFP] to seed ...
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Lactococci and lactobacilli as mucosal delivery vectors for therapeutic proteins and DNA vaccines
... Recombinant BLG is expressed mainly in denatured form with E. coli or L. lactis, whereas its production, in eukaryo- tic cells, is in the native conformation [31,70]. L. lactis strains have been used to deliver an ...
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Improvement of hepatitis B virus DNA vaccines by plasmids coexpressing hepatitis B surface antigen and interleukin-2.
... DISCUSSION In this study, we have constructed plasmid expression vec- tors encoding various forms of HBV envelope proteins and have shown that animals vaccinated with these plasmid vectors develop long-lasting ...
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Plasmids, Lambda based vectors and derivatives. Jamia Hamdard University, New Delhi. Dr Mohan Chandra Joshi, Assistant Professor, Jamia Millia
... Plasmid vectors To carry and replicate the DNA fragment for various purposes, a molecular vehicle is needed in the process of molecular ...of DNA fragments with the help of molecular cloning ...
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Adaptor plasmids simplify the insertion of foreign DNA into helper-independent retroviral vectors.
... Since the genome of the avian sarcoma virus that was used as the basis for the vector construction contains sites for most of the common restriction endo- nucleases, inserting a polylink[r] ...
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Inheritance of Mitochondrial DNA and Plasmids in the Ascomycetous Fungus, Epichloë typhina
... Maternal transmission of all plasmids was observed in 76 progeny; two progeny possessed Bergell and Callan-a, but had the maternal Aubonne- a replaced with the related pat[r] ...
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Male transmission of linear plasmids and mitochondrial DNA in the fungus Neurospora.
... In crosses showing an RFLP in mtDNA, we have shown that some progeny acquiring male plasmids also contain the male RFLP morph.. In some cases mixtures of male and female m[r] ...
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Synthesis of FinP RNA by Plasmids F and pSLT Is Regulated by DNA Adenine Methylation
... pSLT DNA sequenced correspond to the EcoRI-PvuII ...of DNA se- quences are shown at the ...from DNA sequence analysis and later confirmed by fusion ...
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Synapsis-mediated fusion of free DNA ends forms inverted dimer plasmids in yeast.
... Finally, only one of 24 Leu+ transformants examined by DNA gel-transfer hybridization (Figure 5) displayed plasmid restriction fragments consistent with the presence of a [r] ...
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Novel densitometric method for endonuclease analysis of Escherichia coli DNA samples containing multiple plasmids
... The molecular weight of each digested fragment based on the scanning graph was estimated, and then the theoretical concentration ratio of each fragment was calculated based on the assump[r] ...
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Application of filtration blocking models to describe fouling and transmission of large plasmids DNA in sterile filtration
... Figure 1- The transmission of pQR150 (20 kb) through 0.22 m PVDF membranes at 5 and 8 psi constant pressure filtration. The plasmids were collected in batches and the total transmission was determined by the ...
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