Dominant-Negative Mutant
Dominant negative mutant Cyclin T1 proteins inhibit HIV transcription by specifically degrading Tat
... First, mutant Cdk9 proteins defective in kinase activity have been shown to inhibit HIV transcription in cell cul- ture systems ...and mutant Cdk9 which inhibited HIV replication up to 90% ...
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Human piebald trait resulting from a dominant negative mutant allele of the c kit membrane receptor gene
... autophosphorylation of the protein product and causes more extensive depigmentation than "null" mutations. In accord with this "dominant negative" effect, the identical mutation in this ...
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In vitro resolution of adeno-associated virus DNA hairpin termini by wild-type Rep protein is inhibited by a dominant-negative mutant of rep.
... We expressed both wild-type Rep78 and mutant Rep78NTP proteins in two helper-free expression systems consisting of either recombinant baculoviruses in insect cells or the human immunodef[r] ...
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Human insulin like growth factor I receptor function in pituitary cells is suppressed by a dominant negative mutant
... receptors, and WT human IGF-I holotetramers. Both mutant and hybrid receptors were degraded slower than 950Tyr (WT) receptors (> 16 h). Despite their markedly increased ligand binding and prolonged receptor ...
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A Dominant-Negative Mutant of rab5 Inhibits Infection of Cells by Foot-and-Mouth Disease Virus: Implications for Virus Entry
... Foot-and-mouth disease virus (FMDV) can use a number of different integrins ( ␣ v  1, ␣ v  3, ␣ v  6, and ␣ v  8) as receptors to initiate infection. Infection mediated by ␣ v  6 is known to occur by clathrin- ...
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Inactivation of sortilin (a novel lysosomal sorting receptor) by dominant negative competition and RNA interference
... a dominant-negative mutant and by the use of RNAi. Mutant sortilin lacking the carboxyl- terminal region that contains the sorting signal abolished the trafficking of SAPs to the ...
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The Cellular Chaperone Hsc70 Is Specifically Recruited to Reovirus Viral Factories Independently of Its Chaperone Function
... that dominant negative mutants of Hsc70 were also recruited to VFLs, indicating that Hsc70 recruitment to VFLs is independent of the chaperone ...a dominant negative mutant of Hsc70, ...
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African Swine Fever Virus IAP-Like Protein Induces the Activation of Nuclear Factor Kappa B
... African swine fever virus (ASFV) encodes a homologue of the inhibitor of apoptosis (IAP) that promotes cell survival by controlling the activity of caspase-3. Here we show that ASFV IAP is also able to activate the ...
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Development of a counterselectable seamless mutagenesis system in lactic acid bacteria
... the dominant-negative mutant protein PheS* with an A312G amino acid substi- tution rendered cells sensitive to 15 mM p-Cl-Phe in ...a negative selectable marker, a high-efficiency seamless ...
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Transcriptional regulation of long-term memory in the marine snail Aplysia
... a dominant negative mutant of ApAF which cannot be phosphorylated at Ser-266 completely blocked both LTF induced by 5 × 5-HT and that by the ApC/EBP overexpression combined with 1 × ...this ...
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Unabated Adenovirus Replication following Activation of the cGAS/STING-Dependent Antiviral Response in Human Cells
... active mutant, mu1 (H61K/C93S/C96S), that retains its capacity to dimerize with wild-type APOBEC-1 was found to inhibit the editing activity of the latter and was thus a dominant negative ...the ...
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Endocytic pathways mediating oligomeric Aβ42 neurotoxicity
... expressing dominant-negative proteins, or knock-down of endogenous ...the dominant-negative dynamin mutant K44A (myc-tag), the dominant-negative AP180 mutant ...
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An investigation into the combined effects of β-amyloid toxicity and cerebral ischemia on the pathological expression of gangliosides.
... (1997) Expression of a dominant negative mutant of interleukin- 1h converting enzyme in transgenic mice prevents neuronal cell death induced by trophic factor withdrawal and ischemic br[r] ...
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Dynamin- and Clathrin-Dependent Endocytosis in African Swine Fever Virus Entry
... dynamin dominant negative mutant Dyn-K44A, which was previously described to have decreased GTPase activity resulting in reduced endocytosis ...K44A mutant to inhibit endocytosis in Vero cells ...
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A Dominant-Negative Herpesvirus Protein Inhibits Intranuclear Targeting of Viral Proteins: Effects on DNA Replication and Late Gene Expression
... d105 dominant-negative mutant form of the herpes simplex virus 1 (HSV-1) single-stranded DNA- binding protein, ICP8 (d105 ICP8), inhibits wild-type viral replication, and it blocks both viral DNA ...
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Survivin – biology and potential as a therapeutic target in oncology
... function-specific dominant-negative proteins/ peptides/small molecule inhibitors that are capable of abolishing the interactions between survivin and its dif- ferential binding partners (Figure ...using ...
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Domain Structure of Lassa Virus L Protein
... a T7 promoter (T7p) and an IRES to facilitate cap-independent translation. Inserted sequences are shown in gray boxes. Amino acid residues at the site of insertion (X) or split (X ⫺2 X ⫺1 ⱍ Z ⫹1 Z ⫹2 ) were duplicated, ...
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An arginine to histidine mutation in codon 311 of the C erbA beta gene results in a mutant thyroid hormone receptor that does not mediate a dominant negative phenotype
... this mutant allele and, like the father, was clinically ...and mutant alleles in patient ...detectable dominant negative activity in a transfection ...
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Bovine papillomavirus type 1 E1 and simian virus 40 large T antigen share regions of sequence similarity required for multiple functions.
... Generation of E1 mutants. To generate the amino acid substitutions in the E1 open reading frame, oligonucleotide-directed site-specific mutagenesis was per- formed as previously described (14). Briefly, fragments of ...
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The forkhead transcription factor Foxo1 (Fkhr) confers insulin sensitivity onto glucose 6 phosphatase expression
... of dominant neg- ative Foxo1 mutant results in partial inhibition of dex/cAMP–induced G6p and Pepck expression in primary cultures of mouse hepatocyes and kidney LLC-PK1-FBPase + ...
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