Dot-Blot
Use of a Reverse Dot Blot Procedure To Identify the Presence of Multiple Serovars in Chlamydia trachomatis Urogenital Infection
... We are studying chlamydia transmission between sexual contacts and reinfection patterns in a large group of adolescent women where unambiguous identification of serotypes and detection of mixed infection are essential. ...
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Original Article Reverse dot blot hybridization for detection of gyrA mutation for rapid diagnosis of Mycobacterium tuberculosis resistant to fluoroquinolones
... The reverse dot blot hybridization (RDBH) assay has been widely performed in the spoligotyping technique for M. tuberculosis molecular geno- typing identification [14, 15]. Similarly, the method has been ...
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Molecular Epidemiology of Oral Treponemes Associated with Periodontal Disease
... Periodontitis, a disease responsible for tooth loss worldwide, is characterized by chronic inflammation of the periodontium, eventually leading to destruction of periodontal ligaments and supporting alveolar bone. Spi- ...
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I., S LOVÁ EK J., Z EMÁNKOVÁ M. (2002): Utilisation of immu- nochemical methods for detection of Colletotrichum spp. in strawberry . Plant Protect. Sci., 38 : 55–63.
... PTA-ELISA, dot blot, immunoprint and immunofluorescent microscopy were used to test the specifity and sensitivity of ...PTA-ELISA, dot blot and immunoprint in roots, crowns, petioles and ...
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Analysis of differentially expressed genes in two immunologically distinct strains of Eimeria maxima using suppression subtractive hybridization and dot blot hybridization
... Real-time validation of the genes identified by SSH To verify that genes identified by SSH combined with dot-blot hybridization were differentially expressed in the SH and NT strains, real-time PCR was ...
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Validation of a Multiplex Pneumococcal Serotyping Assay with Clinical Samples
... the dot blot assay using the polyclonal rabbit antisera and MAb ...positive dot blot results with a serogroup 18-specific polyclonal rabbit serum or with MAb Hyp18CM1 ...
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Detection and Identification of Fungi from Fungus Balls of the Maxillary Sinus by Molecular Techniques
... specimens. Dot blot hybridization and se- quence analysis were studied in 31 tissue samples from patients with histologically proven fungus ...for dot blot ...
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A Simple Method for Detection of Multiple Chemical Specific IgGs in Serum Based on Dot Blotting
... by dot blot assay using X-HSA ad- ducts as the ...by dot blot assays and that chemical-HSA adducts generated under alkaline conditions could be used as ...
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Serum HER2 levels are increased in cats with mammary carcinomas and predict tissue HER2 status
... After testing for normality, the Wilcoxon test was used to compare the sHER2 levels between cats with mammary carcinoma and healthy ones, and also to compare sHER2 levels and tissue HER2 status among cancer group cats. ...
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Mycobacterium malmoense Specific Nested PCR Based on a Conserved Sequence Detected in Random Amplified Polymorphic DNA Fingerprints
... Dot blot hybridization. Dot blot hybridizations of mycobacterial chromosomal DNA were carried out by modifying the published protocol ...each dot on an Immo- bilon-N membrane ...
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Screening for active toxoplasmosis in patients by DNA hybridization with the ABGTg7 probe in blood samples
... phate, 1 mmol (per liter) each of the oligonucleotides 1 (59-GGAACTGCATC CGTTCATGAG-39; positions 694 to 714) and 4 (59-TCTTTAAAGCGTTCGT GGTC-39; positions 887 to 868) (5), and 1 mg of the DNA to be amplified. The ...
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Diagnosis of Human Leptospirosis by Monoclonal Antibody Based Antigen Detection in Urine
... Specimens. Two sets of clinical specimens were used in this study. The first set was from 36 presumptively diagnosed leptospirosis patients admitted to Khon Kaen Provincial Hospital (about 390 km northeast of Bangkok, ...
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IN VITRO EVALUATION OF ANTIMICROBIAL AND ANTIOXIDANT ACTIVITIES OF METHANOL EXTRACTS OF JASMINUM CUSPIDATUM (L )
... In this communication we report the antioxidant, antimicrobial activities of the methanol extracts of J. cuspidatum leaves. The total phenol content of methanol extracts of leaves was recorded 289 mg/g Dry Weight of ...
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The role of apolipoprotein N acyl transferase, Lnt, in the lipidation of factor H binding protein of Neisseria meningitidis strain MC58 and its potential as a drug target
... immuno-dot blot using JAR4 was performed from standardised cell ...immuno-dot blot and our immunofluorescence microscopy results, the level of antibody binding of strain MC58Lnt was ...
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DIFFERENT TYPES OF ANTIOXIDANT ACTIVITY OF AERVA PERSICA (BURM F ) MERR
... like; dot blot DPPH method, DPPH scavenging activity, ABTS method, reducing power assay, OH radical scavenging assay, nitric oxide scavenging assay, total antioxidant activity (Phosphomolybdenum (PMo) ...
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