Double Stranded RNA Inhibition
Inhibition of the double-stranded RNA-dependent protein kinase PKR by mammalian ribosomes
... interferon-inducible, double-stranded RNA (dsRNA)- dependent protein kinase PKR is an important factor for the regulation of protein synthesis in response to viral infections and following exposure ...
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Inhibition of binding to initiation complexes of nascent reovirus mRNA by double-stranded RNA-dependent protein kinase.
... experiments designed to examine whether the binding of nascent reovirus mRNA to initiation complexes was prefer- entially inhibited in the coupled, cell-free system had to be carried out[r] ...
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Double-Stranded RNA Binding by Human Cytomegalovirus pTRS1
... One early response to acute viral infections is the production of alpha/beta interferons by the host immune system. These interferons induce transcription of many genes, including pro- tein kinase R (PKR) and 2-5 ...
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Identification of an RNA Silencing Suppressor from a Plant Double-Stranded RNA Virus
... Taken together, these results indicate that Pns10 had a syn- ergetic effect on PVX infection in N. benthamiana. Further- more, this effect was much more prominent in systemically infected leaves, presumably as a result ...
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Cytoplasmic Granule Formation and Translational Inhibition of Nodaviral RNAs in the Absence of the Double-Stranded RNA Binding Protein B2
... FHV RNA replication in BHK21 cells is vigorous in the absence of B2, suggesting that these cells do not use RNA interference as an antiviral pathway ...viral RNA synthesis, it leads to defects in the ...
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Inhibition of a Large Double-Stranded DNA Virus by MxA Protein
... single-stranded RNA viruses, as well as double-stranded RNA viruses, positive-sense single-stranded RNA viruses, and the reverse-transcribing DNA virus hepatitis B ...
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Double-stranded RNA-activated protein kinase PKR of fishes and amphibians: Varying the number of double-stranded RNA binding domains and lineage-specific duplications
... The best-characterized substrate of PKR is the α subunit of eukaryotic translation initiation factor 2 (eIF2), which is phosphorylated at Ser51. Phosphorylation of eIF2α is one of the best-understood mechanisms enabling ...
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Molecular details of the double-stranded RNA virus replication and assembly
... Efficient Inhibition of Avian and Seasonal Influenza A Viruses by a Virus-Specific Dicer-Substrate Small Interfering RNA Swarm in Human Monocyte-Derived Macrophages and Dendritic ...
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Inhibition of Antiviral Innate Immunity by Birnavirus VP3 Protein via Blockage of Viral Double-Stranded RNA Binding to the Host Cytoplasmic RNA Detector MDA5
... Our data demonstrate that VP3 inhibits antiviral innate immunity by blocking binding of viral genomic dsRNA to MDA5. IMPORTANCE MDA5, a known pattern recognition receptor and cytoplasmic viral RNA sensor, plays a ...
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Double-stranded RNA is a trigger for apoptosis in vaccinia virus-infected cells.
... VAI RNA to PKR ...Whether inhibition of eIF-2a phos- phorylation is not sufficient to prevent induction of apoptosis or whether the effects of the K3L gene products are overcome in VVDE3L-infected cells is ...
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Down-Regulation of p53 by Double-Stranded RNA Modulates the Antiviral Response
... Our results and the observations of others raise concerns about the use of viruses, either as oncolytic viruses or as gene therapy vectors, in the treatment of cancer. Virus vectors, usually replication-deficient ...
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A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA.
... Ethidium bromide-stained agarose gel (A) and autoradio- gram (B) of the dsRNAs contained in purified VLPs from strain RE455 by cesium chloride density gradient centrifugation and the pro[r] ...
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Identification of double-stranded RNA-binding domains in the interferon-induced double-stranded RNA-activated p68 kinase
... To further define the domains responsible for binding to dsRNA, we cloned the mouse dsRNA-activated p65 kinase and used sequence align- ment to identify conserved domains in the N-termin[r] ...
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Poliovirus single-stranded RNA and double-stranded RNA: differential infectivity in enucleate cells.
... Two types of control nucleate cells were prepared for these experiments: (i) normal cells never treated with cytochalasin B and (ii) cells exposed to 10 ,ug of cytochalasin B per ml in a[r] ...
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Modification of retroviral RNA by double-stranded RNA adenosine deaminase.
... since RNA from pCN-180 a s, which lacks cis viral se- quences, did not show any modifications, whereas RNA from pLAG a sPE, which encodes retroviral sequences, was modi- ...genomic RNA by dsRAD could ...
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Template Role of Double-Stranded RNA in Tombusvirus Replication
... Positive-stranded RNA viruses of plants use their RNAs as the templates for ...complete RNA replication of Tomato bushy stunt virus (TBSV) in yeast cell-free extracts and in plant ...a ...
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Overlapping genes in a yeast double-stranded RNA virus.
... We examined, in two ways, the question of whether this second open reading frame is expressed: by searching for a polypeptide with predicted epitopes in viral particles and by in vitro t[r] ...
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Microglia Recognize Double-Stranded RNA via TLR3
... Microglia Recognize Double-Stranded RNA via TLR3 1 Terrence Town,* David Jeng,* Lena Alexopoulou, ‡ Jun Tan, § and Richard A. Flavell 2 * † Microglia are CNS resident innate immune cells of myeloid ...
Double-Stranded RNA of the Diagnosis of Citrus and Avocado Viruses
... Until recently presence of homogeneous segments of double-stranded (ds)RNA, corresponding to twice the size of viral genomic ssRNA, in infected plants have been over[r] ...
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Double-Stranded RNA Is Detected by Immunofluorescence Analysis in RNA and DNA Virus Infections, Including Those by Negative-Stranded RNA Viruses
... mechanism(s) of generation of overlapping transcripts in parvo- virus infection awaits further study. IAV transcription and replication occur in the nucleus inside a helical nucleocapsid. While diffuse dsRNA ...
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