Droplet Digital PCR
Clinical Utility of Droplet Digital PCR for Human Cytomegalovirus
... quantitative PCR (qPCR) assay targets two genes in CMV simultaneously, the UL55 and UL123 genes, to increase the likelihood of accurately detecting all possible clinical ...ddPCR. Droplet digital ...
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Detection of Methicillin Resistant Staphylococcus aureus by a Duplex Droplet Digital PCR Assay
... a droplet digital PCR (ddPCR) assay, which is a next-generation emulsion-based endpoint PCR assay for high-precision MRSA ...Quantitative PCR (qPCR) (Roche Light Cycler 480) and ddPCR ...
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Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant, soil and water samples
... quantitative PCR has enabled high-throughput detection and quantification of pathogens; however, its quantitative use is linked to standardized reference materials, and its sensitivity to inhibitors can lead to ...
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Original Article Detection of IDH1 and TERT promoter mutations with droplet digital PCR in diffuse gliomas
... [18] Hindson BJ, Ness KD, Masquelier DA, Belgrad- er P, Heredia NJ, Makarewicz AJ, Bright IJ, Lu- cero MY, Hiddessen AL, Legler TC, Kitano TK, Hodel MR, Petersen JF, Wyatt PW, Steenblock ER, Shah PH, Bousse LJ, Troup CB, ...
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Determination of copy number and circularization ratio of Tn916 Tn1545 family of conjugative transposons in oral streptococci by droplet digital PCR
... by droplet digital PCR (ddPCR) as it has been shown to produce more precise and reproducible results when compared to qPCR ...single PCR reaction is divided into approxi- mately 20,000 ...
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Diagnostic accuracy of droplet digital PCR for detection of EGFR T790M mutation in circulating tumor DNA
... Karlovich et al also revealed that the sensitivity and specific- ity of BEAMing was 73.3% and 50.0%. 34 Hence, the ddPCR Figure 2 Meta-analysis of (A) sensitivity, (B) specificity, (C) positive likelihood ratio, (D) ...
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A Direct Droplet Digital PCR Method for E coli Host Residual DNA Quantification
... needed. Droplet digital PCR (ddPCR) is a new method where the reaction is partitioned into about 20,000 nanoliter-sized droplets and each droplet acts as individual PCR reac- ...
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Diagnostic and prognostic microRNAs in the serum of breast cancer patients measured by droplet digital PCR
... Background: Breast cancer circulating biomarkers include carcinoembryonic antigen and carbohydrate antigen 15 – 3, which are used for patient follow-up. Since sensitivity and specificity are low, novel and more useful ...
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Non-invasive fetal sex diagnosis in plasma of early weeks pregnants using droplet digital PCR
... new droplet digital PCR (ddPCR) technology for analysis of ...emulsion droplet technology permitting the precise quantification of rare target nucleic acids in the sample (Pinheiro et ...
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A robust internal control for high-precision DNA methylation analyses by droplet digital PCR
... QX200™ Droplet Digital™ PCR System (Bio-Rad) was used for ...QX200 Droplet Generator (Bio-Rad), using 20 μl of the ddPCR mixture and 70 μl droplet generation oil ...96-well PCR ...
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Droplet Digital PCR Based Detection of Clarithromycin Resistance in Helicobacter pylori Isolates Reveals Frequent Heteroresistance
... ABSTRACT Chronic infection with Helicobacter pylori causes peptic ulcers and stom- ach cancer in a subset of infected individuals. While standard eradication therapy in- cludes multiple antibiotics, treatment failure due ...
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A droplet digital PCR detection method for rare L1 insertions in tumors
... Retrotransposition of long interspersed element 1 (L1) in human germline and somatic cells contributes to gen- omic variation in human populations and is implicated in tumorigenesis. In this study, we designed ...
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Copy number variability of expression plasmids determined by cell sorting and Droplet Digital PCR
... accurate Droplet Digital PCR (ddPCR), and to reveal relationships between copy number and the degree of heterogeneity as determined by EGFP ...optimal droplet occupation) ...
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Quantification of Zoonotic Bacterial Pathogens within Commercial Poultry Processing Water Samples Using Droplet Digital PCR
... generation PCR technology, known as droplet digital PCR (ddPCR) has been devel- oped to be more accurate and sensitive to detect genetic targets than current quantification methods, but this ...
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Accurate Detection of Methicillin Resistant Staphylococcus aureus in Mixtures by Use of Single Bacterium Duplex Droplet Digital PCR
... 22. Hindson BJ, Ness KD, Masquelier DA, Belgrader P, Heredia NJ, Makar- ewicz AJ, Bright IJ, Lucero MY, Hiddessen AL, Legler TC, Kitano TK, Hodel MR, Petersen JF, Wyatt PW, Steenblock ER, Shah PH, Bousse LJ, Troup CB, ...
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Comparison of Droplet Digital PCR to Real Time PCR for Quantitative Detection of Cytomegalovirus
... real-time PCR (QRT-PCR) has been widely implemented for clinical viral load testing, but a lack of standardization and relatively poor precision have hindered its ...usefulness. Digital PCR ...
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IDH1 mutation detection by droplet digital PCR in glioma
... Droplet digital PCR (ddPCR) is one of the latest molecular amplification techniques that offers high precision and sensitivity and detects rare alleles, copy number variations and absolute ...
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Quantitation of Integrated HIV Provirus by Pulsed Field Gel Electrophoresis and Droplet Digital PCR
... utilized droplet digital PCR (ddPCR), which is a sensitive and precise platform for quantifying DNA and RNA sequences of multiple viral pathogens, including HIV and herpesviruses (15, ...
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Killer-cell Immunoglobulin-like Receptor gene linkage and copy number variation analysis by droplet digital PCR
... by PCR- SSP that WJR076 carries the hybrid gene KIR2DL5/3DP1 (known as KIR3DP1*004), which has been previously iden- tified on various extended KIR haplotypes ...
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Identification of genomic aberrations in hemangioblastoma by droplet digital PCR and SNP microarray highlights novel candidate genes and pathways for pathogenesis
... and PCR reaction mixtures were partitioned into an emulsion of approxi- mately 20,000 droplets using the QX100 droplet gener- ator (Bio-Rad, ...96-well PCR plate, heat sealed, and placed in a conven- ...
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