E coli-expressed enzyme
EFFECTS OF MTN ENZYME DEFICIENCY ON E. COLI O157:H7 GROWTH AND VIRULENCE
... in E. coli O157:H7 Creation of the MTN gene knock-out strain of ...E. coli O157:H7 was achieved using the λ-red recombination ...and expressed MTN under the control of an IPTG inducible ...
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Comparative Analysis of Peripheral Alkaline Phytase Protein Structures Expressed in E. coli
... the enzyme activities, thermostability, and yields need to be increased; therefore, it is desirable to isolate the phytase gene from species of ...the enzyme is more suited to in-feed application than ...
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Construction of a chimeric CotA protein bearing glutathione S transferase for improved enzyme activity
... bifunctional enzyme was constructed by fusing catalytic conserved region of CotA from Bacillus subtilis to glutathione S-transferase (GST) to produce a chimera exhibiting both laccase and GST ...activities. ...
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Molecular cloning and host optimization study for enhanced expression of thermostable protease to meet the commercial demand
... study E. coli C41 and E. coli Rosetta were reported for maximum protein expression under IPTG ...The enzyme had shown excellent proteolytic activity and activity remained in the ...
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Sero diagnostic evaluation of Toxoplasma gondii recombinant Rhoptry antigen 8 expressed in E coli
... than enzyme-linked immunosorbent assay (ELISA), especially considering that the likelihood of obtaining false positive results by western blot is much lower as compared to ELISA ...
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Extracellular overexpression of recombinant Thermobifida fusca cutinase by alpha-hemolysin secretion system in E. coli BL21(DE3)
... and expressed in ...purified enzyme, which had an a-hemolysin secretion pathway signal peptide attached, had substrate specificity, pH and temperature profile, as well as application capability in ...
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Engineering E. coli–E. coli cocultures for production of muconic acid from glycerol
... and E. coli strains for MA production from ...pYfsZ expressed the aroZ and shiA enzyme in the form of a fusion protein through a flexible glycine-serine con- necting ...desired E. ...
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Characterization of cinnamyl alcohol dehydrogenase of Helicobacter pylori An aldehyde dismutating enzyme
... chia coli DH5a and the pET-Hp1104 construct containing the cloned gene was transformed into ...E. coli BL21(DE3)plysS for ...transformed E. coli BL21(DE3)plysS typically yielded ...
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Influence of Anti FloR Antibody on Florfenicol Accumulation in Florfenicol Resistant Escherichia coli and Enzyme Linked Immunosorbent Assay for Detection of Florfenicol Resistant E coli Isolates
... Escherichia coli isolates by enzyme-linked immunosorbent assay (ELISA), anti-FloR1 antibodies were produced in mice using a recombinant glutathione S-transferase (GST)-FloR1 protein, which was ...
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Structural Insights into the Recovery of Aldolase Activity in N -Acetylneuraminic Acid Lyase by Replacement of the Catalytically Active Lysine with γ-Thialysine by Using a Chemical Mutagenesis Strategy
... Chemical modification has been used to introduce the unnatu- ral amino acid g-thialysine in place of the catalytically impor- tant Lys165 in the enzyme N-acetylneuraminic acid lyase (NAL). The Staphylococcus ...
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Towards creating an extended metabolic model (EMM) for E. coli using enzyme promiscuity prediction and metabolomics data
... C4 consists of four predicted reactions that are not currently catalogued in KEGG for any organism (Fig. 5). The first reaction (Fig. 5a) is the oxidoreductive interconversion between aminomalonate and l-serine by EC ...
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Necrotizing Enterocolitis with Escherichia coli Heat-Labile Enterotoxin
... Of affected babies, 15 had toxigenic Escherichia coli or heat-labile E coli toxin in feces de-.. tected by enzyme-linked immunosorbent assay.[r] ...
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GM1 erythroimmunoassay for detection and titration of Escherichia coli heat labile enterotoxin
... Method for the detection and dosage of the Escherichia coli heat-labile enterotoxin by an enzyme-linked immunosorbent assay using a new simple kit test.. Production of "Escherichia coli"[r] ...
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Effect of carbaryl on enzyme activities in Escherichia coli and soil isolate Pseudomonas aeruginosa
... the enzyme activities of Escherichia coli and Pseudomonas aeruginosa on exposure to ...Escherichia coli cells revealing that immobilized system is less sensitive to the ...Escherichia coli and ...
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Activity of avian retroviral protease expressed in Escherichia coli.
... The construct coding for the mutant PR [pNC-PRS-I] was Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The 3' end of the avian sarcoma leukosis virus ASLV gag gene enco[r] ...
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Antibodies against outer capsid proteins of grass carp reovirus expressed in E coli are capable of neutralizing viral infectivity
... a major antigen determinant. Given this result, it is pro- posed that VP7 may act as a cell attachment protein to bind to the cell receptor, because VP7 is located at the outermost layer of the particle. The effective ...
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High Yield Overexpression, Refolding, Purification and Characterization of Pseudomonas aeruginosa Type B-Flagellin: An Improved Method Without Sonication
... the E. coli expression vector pET28a, in frame with a T7 promoter, kanamycin resistant gene and the C-terminal six-His-tagged ...into E. coli Top10F competent cells, transformants were ...
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Role of Cell Surface Structures in Biofilm Formation by Escherichia coli
... [35], E. coli are serotyped on the basis of their O (somatic), H (flagellar), and K (capsular) surface antigen profiles (185, ...an E. coli strain that was inagglutinable by O antiserum but ...
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Enzyme capture assay for rapid identification of Escherichia coli in blood cultures
... An enzyme capture assay ECA for rapid identification of Escherichia coli in blood cultures by using to capture this enzyme from the cell lysates of blood cultures which showed growth of [r] ...
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Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response
... Escherichia coli, and Staphylococcus aureus are the most common causes of meningitis in newborns and adults ...pathogenic E. coli, an important zoonotic bac- terial pathogen, has been widely reported ...
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