E1 protein
The E1 Protein of Human Papillomavirus Type 16 Is Dispensable for Maintenance Replication of the Viral Genome
... exogenous E1 expression were ex- posed to a high calcium ...exogenous E1 expression (Fig. 4B, lanes 1 to 2). However, the E1-defective HPV16 genomes were amplified only in the presence of exogenous ...
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Nuclear Import of Bovine Papillomavirus Type 1 E1 Protein Is Mediated by Multiple Alpha Importins and Is Negatively Regulated by Phosphorylation near a Nuclear Localization Signal
... BPV1 E1 protein to ␣ importins was significantly decreased when a pseudophosphorylation mu- tation was introduced at serine ...mutant E1 protein failed to enter the nu- cleus of ...
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Association of the Human Papillomavirus Type 11 E1 Protein with Histone H1
... to E1 binding. Although protein renaturation was attempted in both cases, the native state of H1 was not ...no protein denaturants, H1 was isolated in its native state in association with intact ...
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Bovine papillomavirus E1 protein binds specifically DNA polymerase alpha but not replication protein A.
... Mouse cell extracts are less active than primate cell extracts for BPV DNA synthesis in vitro. We have first determined whether cell extracts from normal nonpermissive cells (COS-1) were capable of supporting BPV DNA ...
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The Carboxyl-Terminal Region of the Human Papillomavirus Type 16 E1 Protein Determines E2 Protein Specificity during DNA Replication
... virus-encoded E1 and E2 proteins collaborate to target the origin and recruit host DNA replication ...of E1 and E2 proteins support homologous and heterologous papillomaviral origin replication in ...
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Functional Mapping of the DNA Binding Domain of Bovine Papillomavirus E1 Protein
... E1DBD mutants positive for E2 interaction support partial in vivo DNA replication. The ability of some binding-defective mutants to be rescued by E2 suggested that they may retain sufficient function to support DNA ...
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Cell-based analysis of Chikungunya virus E1 protein in membrane fusion
... changes E1’s fusion dependencies on pH and cholesterol (Figure ...influence E1 fusogenic capacity (Figure ...CHIKV E1-V178A mutant exhibits changes in pH-dependence, but no significant differences in ...
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Recombinant Listeria monocytogenes vaccination eliminates papillomavirus-induced tumors and prevents papilloma formation from viral DNA.
... viral E1 protein (E1-rLm) to protect outbred rabbits against CRPV- and CRPV DNA-induced ...or E1-rLm and then challenged with CRPV or viral ...of E1-rLm-vaccinated rabbits generated ...
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Prime-Boost Immunization Strategies against Chikungunya Virus
... a protein preparation consisting of a covalently linked CHIKV p62-E1 heterodimer generously provided by Felix Rey of the Pasteur Institute ...The protein antigen and the other CHIKV immunogens are ...
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Chimeric Derivatives of Hepatitis B Virus Core Particles Carrying Major Epitopes of the Rubella Virus E1 Glycoprotein
... recombinant protein was purified by IMAC under denaturing conditions (8 M urea, 5 mM  -ME, and 100 mM Tris-HCl, pH ...RV E1 was eluted in the IMAC elution buffer (8 M urea, 5 mM  -ME, 100 mM Tris-HCl [pH ...
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Role of Metastability and Acidic pH in Membrane Fusion by Tick-Borne Encephalitis Virus
... envelope protein E of the flavivirus tick-borne encephalitis (TBE) virus is, like the alphavirus E1 protein, a class II viral fusion protein that differs structurally and probably ...
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Identification of immunodominant epitopes of e1 and nucleocapsid proteins of chikungunya virus with neutralizing capacity
... with E1/virus lysate antisera, indicate the presence of monoclonal antibody specific to these ...the E1 protein had shown to inhibit the virus assembly during in vitro study (Masrinoul et ...of ...
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Human TATA Binding Protein Inhibits Human Papillomavirus Type 11 DNA Replication by Antagonizing E1-E2 Protein Complex Formation on the Viral Origin of Replication
... of E1 or its ability to enhance E2 binding in their ...BPV-1 E1-mutant viruses had increased transforming and transcriptional activity because regulation from the major early promoters P97 and P89 was ...
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Cell Culture-Adaptive Mutations Promote Viral Protein-Protein Interactions and Morphogenesis of Infectious Hepatitis C Virus
... HCV protein-protein interactions in the cell was described previ- ously ...HCV protein was fused with the Saccharomyces cerevisiae Gal4 DNA-binding domain (Gal4-BD) and the activation domain of the ...
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Oligomerization of Hepatitis C Virus Core Protein Is Crucial for Interaction with the Cytoplasmic Domain of E1 Envelope Protein
... E1 protein. To confirm the presence of the cytoplasmic domain in the E1 protein, in vitro translation products of the HCV core and E1 polyprotein car- rying FLAG and HA tags in the N- ...
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Transcriptional activation function is not required for stimulation of DNA replication by bovine papillomavirus type 1 E2.
... the E1 protein alone can support low-level replication in ...enhances E1-driven replication, defining E2 as a factor which stimulates basal replication as well as transcrip- ...of E1-dependent ...
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Mutational Analysis, Using a Full-Length Rubella Virus cDNA Clone, of Rubella Virus E1 Transmembrane and Cytoplasmic Domains Required for Virus Release
... structural protein synthe- sis and virus release of the mutants and parental BRM34 virus, the amounts of intracellular E1 after 10 min of chase and extracellular E1 after 5 h of chase in each mutant ...
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Interaction between hepatitis C virus core protein and E1 envelope protein.
... the E1 envelope protein was ex- pressed by ...the E1 envelope protein does not cross-react with our anti-core ...the E1 protein was faithfully expressed in this experiment, an ...
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In Vitro and In Vivo Biology of Recombinant Adenovirus Vectors with E1, E1/E2A, or E1/E4 Deleted
... of E1-deleted vectors induced a consistent anti-Ad CTL ...virus protein synthesis should decrease the anti-Ad CTL response, they are consistent with a recent report showing that in human cells endogenous ...
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Serotype-Specific Neutralizing Antibody Epitopes of Human Adenovirus Type 3 (HAdV-3) and HAdV-7 Reside in Multiple Hexon Hypervariable Regions
... hexon protein sequences, and the antisense primers (IMHE1a, IMHE2a, IMHE3a, IMHE4a, HR1r, HR2r, HR3r, and HR4r) were located in the eight mutant regions (E1, E2, E3, and E4 from HAdV-7 strain GZ08 hexon and ...
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