EB Protein Cloning, Expression and Purification
Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
... METHODS Cloning Wolbachia pipientis -DsbA1 Wolbachia pipientis wMel strain was maintained in Drosophila melanogaster yw67c23 flies reared on standard corn flour-sugar-yeast medium at ...
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Cloning, Expression, and Purification of HIV-2 gp125
... the cloning, purification, and characterization of two recombinant forms of the envelope glyco- protein gp125 from a primary HIV-2 SBL-6669 ...
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Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning, Protein Expression, and Purification in Escherichia coli
... fusion protein. To further improve protein solubility the polar amino acids lysine and glutamine can also be used in the ...of protein solubility prediction online software, the solubilities of HspX ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... pET expression system has been developed for the cloning and ex- pression of recombinant proteins in ...sub cloning of 820 bp fHBP gene into pET 28a (+) expression vec- tor was carried out by ...
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Cloning, Expression, Purification, and Crystallization of P aeruginosa ICMP
... the protein remain ...crystallizable protein species is the prerequisite for structural studies by crystallography, we re- ported the successful expression, purification, and crystallization ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... total protein yield at the last purification step was approximately 71 mg of Rep 34 per liter of bacterial cul- ...lated protein migrated as a large homogenous band with a molecular weight between 20 ...
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CLONING, PROKARYOTIC EXPRESSION AND PROTEIN PURIFICATION OF SUGARCANE ENDOGENOUS AZOTOBACTER KLEBSIELLA VARIICOLA DX120E NIFK GENE
... prokaryotic expression protein by induction was detected by mass spectrometry, which had an isoelectric point of ...nifK protein was successfully expressed in a prokaryotic expression system, ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... of protein. So, it was necessary to over- express this protein in recombinant form in ...pET32a expression system was ...for purification of expressed protein. High level of ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... target protein contained 16 additional amino acid residues at the C-terminus, including six histidine residues for purification of the recombinant protein by Ni-NTA ...
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
... sion are suitable because they are able to transport arabinose, but they can not metabolize it [19]. The His-tagged AACT was purified using the Ni-NTA affinity chromatography and a step-wise concentra- tion gradient of ...
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Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius
... acidic protein with a pI of ...a protein of 364 amino acid residues, the first 35 of which constituted the signal ...the protein was predicted to have a molecular mass of ...
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Cloning, expression, purification and in silico analysis of n terminal domain of pneumococcal surface protein a from all six clades
... surface protein A (PspA) is one of the better defined pneumococcal virulence ...repot cloning, expression and purification of all six clades of PspA (extracellular domain ...
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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus
... 20 utilized to lower the imidazole concentration slowly to avoid protein denaturation and precipitation. In size exclusion chromatography, also known as gel filtration chromatography, a column filled with porous ...
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Cloning, expression, purification and characterization of two diagnostically important proteins of Brucella abortus
... bovine population both in farm and in the village animals. Brucellosis is still an uncontrolled serious health problem in many developing countries including India. A large number of tests are available presently for the ...
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Cloning, expression and purification of Mycobacterium tuberculosis ESAT-6 and CFP-10 antigens.
... Immunoblotting of recombinant proteins. Western blot analysis with rabbit antisera was performed for evaluation of the antigenic reactivity of both recombinant proteins. The recombinant proteins were electro-transferred ...
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Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
... Multi-epitopic protein based identification approach, using bioinformatics tools expected to be easier to use for standardization and diagnosis of the infection with more sensitivity and ...after cloning ...
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<p>In silico Analysis, Molecular Docking, Molecular Dynamic, Cloning, Expression and Purification of Chimeric Protein in Colorectal Cancer Treatment</p>
... recombinant protein vac- cines, as opposed to CRC for clinical function, extensive testing is ...Recombinant protein vaccines must be harmless, stable in various conditions, have high immuno- genicity, and ...
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Production of a modified peptide clavanin in Pichia pastoris: cloning, expression, purification and in vitro activities
... carrier protein and the construction was tested using two promoters, P AOX1 and P GAP , based on either induced or constitutive expression systems, ...inducible expression systems produce active ...
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Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
... diagnosis. A fragment of the GRA5 gene was cloned successfully into prokaryotic expression vector. The pGEX6p-1 was used to allow high level expression of GRA5 using tac promoter. The pGEX-6p-1 expres- sion ...
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Cloning and Expression of the algl Gene, Encoding the Azotobacter chroococcum Alginate Lyase: Purification and Characterization of the Enzyme
... The flanking sequences of the A. chroococcum algL gene show a high homology to the recently reported sequence of the algXLIVFA operon from A. vinelandii. Deduced amino acid sequence of AlgL. The deduced protein ...
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