EBNA-3C
EBNA-3B- and EBNA-3C-Regulated Cellular Genes in Epstein-Barr Virus-Immortalized Lymphoblastoid Cell Lines
... an EBNA-3B-deleted BAC mutant containing a kanamycin resistance cassette grow slowly and are hypomorphic for EBNA-3C ...the EBNA-3B ORF from an EBV genome cloned as a BAC re- sulted in viruses ...
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Downregulation of Integrin Receptor-Signaling Genes by Epstein-Barr Virus EBNA 3C via Promoter-Proximal and -Distal Binding Elements
... of EBNA 3C on cellular- gene expression was carried out using stable transfectants of a B-cell line that express higher levels of EBNA 3C than are present in latently infected cells (50; also ...
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EBNA-2 and EBNA-3C extensively and mutually exclusively associate with RBPJkappa in Epstein-Barr virus-transformed B lymphocytes.
... rabbit EBNA-2 (E2) antibody, 50 m l of monoclonal D8 (EBNA-3C [E3c]) antibody, 10 m l of monoclonal JF186 (EBNA-LP [E-LP]) antibody, or no anti- body ( f ) (12, 16, 20, ...left. EBNA-2 ...
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Epstein-Barr Virus EBNA-3C Is Targeted to and Regulates Expression from the Bidirectional LMP-1/2B Promoter
... of EBNA-3C, levels of LMP-1 remain high ...however, EBNA-3C can mediate either repression or activation of the LMP-1 pro- moter through J and ...of EBNA-3C on the LMP-1 ...
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Epstein-Barr virus nuclear proteins EBNA-3A and EBNA-3C are essential for B-lymphocyte growth transformation.
... The rapid loss of the MT EBNA-3A recombinant genomes from the initially coinfected LCLs must involve not only a specific selection against cells infected with the mutant recombinant geno[r] ...
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Epstein-Barr virus types 1 and 2 differ in their EBNA-3A, EBNA-3B, and EBNA-3C genes.
... We determined the DNA sequence of the AG876 EBV-2 EBNA-3 coding region and have compared it with known B95-8 EBV-1 EBNA-3 sequences to delineate the extent of divergence between EBV-1 an[r] ...
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Epstein-Barr Virus Nuclear Antigen 3C Activates the Latent Membrane Protein 1 Promoter in the Presence of Epstein-Barr Virus Nuclear Antigen 2 through Sequences Encompassing an Spi-1/Spi-B Binding Site
... by EBNA-2 and EBNA-3C is reminiscent of the interaction of both EBV proteins with ...the EBNA-3 proteins is to reg- ulate the activity of EBNA-2. EBNA-3C is thus far ...
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Popp, Claudia (2007): Die Rolle der Epstein-Barr Virus nukleären Antigene 3A und 3C in der B-Zellimmortalisierung. Dissertation, LMU München: Fakultät für Biologie
... Meine Beobachtungen unterscheiden sich von den gerade beschriebenen. Das Auf- treten erster Klumpen wurde bei Wildtyp-Virus 2089 und ∆EBNA-3C Virus zeitgleich beobachtet. Auch kann das geringere Wachstum ...
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Epstein-Barr Virus Nuclear Antigen 3C Putative Repression Domain Mediates Coactivation of the LMP1 Promoter with EBNA-2
... of EBNA-3C aa 365 to 545 in LMP1 promoter coactivation with EBNA-2, we screened a panel of EBNA-3C-interacting proteins obtained from a yeast two-hybrid screen for dependence on this ...
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A conserved domain of the Epstein-Barr virus nuclear antigens 3A and 3C binds to a discrete domain of Jkappa.
... of EBNA-3C is important in the inter- action with J k ...of EBNA-3C are con- served in the other two EBNA-3 family members ...of EBNA-3C to repress EBNA-2-me- ...
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Löfqvist, Madelaine (2004): The role of Epstein-Barr virus nuclear antigen 3C in the immortalisation process of primary human B-lymphocytes.. Dissertation, LMU München: Fakultät für Biologie
... Therefore, new methods had to be developed for faster and more efficient mutagenesis of large DNA fragments. The first cloning vectors for large DNA fragments were yeast artificial chromosomes (YACs), which are ...
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Rep*: a Viral Element That Can Partially Replace the Origin of Plasmid DNA Synthesis of Epstein-Barr Virus
... 1925 and 1926, the two plasmids behave similarly. In fact, during the time course of this experiment, the absolute amount of replicated 1925 and 1926 would have increased more than 2,500-fold, were all progeny cells to ...
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Expression of Epstein-Barr Virus Nuclear Antigen 1 Is Associated with Enhanced Expression of CD25 in the Hodgkin Cell Line L428
... (c). EBNA-1-expressing L428 cells or vector control cells were stained with isotype controls and corresponding PE-conjugated antibodies directed against CD25, CD122, and IL-2 g ...shading). ...
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Functional domains of Epstein-Barr virus nuclear antigen EBNA-1.
... To identify the EBNA-1 nuclear localization signal, small oligonucleotides representing EBNA-1 sequences that encode clusters of basic peptides were transferred into two different vector[r] ...
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Epstein-Barr Virus Nuclear Protein 2 Has at Least Two N-Terminal Domains That Mediate Self-Association
... of EBNA-2 required for association with F194 by transiently expressing wild-type EBNA-2 or EBNA-2 deletion mutants in BJAB cells that sta- bly express F194 ...the EBNA-2 deletion mutants are ...
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Epstein-Barr virus latent membrane protein (LMP1) and nuclear proteins 2 and 3C are effectors of phenotypic changes in B lymphocytes: EBNA-2 and LMP1 cooperatively induce CD23.
... The 5' EcoRI- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest the Louckes B-lymphoma cell line, LMP1 induced broad phenotypic changes, including growth in large tight c[r] ...
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The Epstein-Barr virus EBNA-1 promoter Qp requires an initiator-like element.
... sufficient EBNA-1 is avail- able in the cycling cell to mediate DNA ...mediated EBNA-1 expression during type I and II latency, Qp-mediated expression of EBNA-1 would automatically be activated if ...
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Multi-frequency analysis of the two CSS quasars 3C 43 & 3C 298
... 3C 43 is an object hard to interpret. It is dominated by a knotty jet showing several large bends which cannot be ex- plained by projection effects if the overall source orientation is > ∼ 30 ◦ , as deduced ...
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A Single Amino Acid in EBNA-2 Determines Superior B Lymphoblastoid Cell Line Growth Maintenance by Epstein-Barr Virus Type 1 EBNA-2
... 6 EBNA-2 ChIP binding at CXCR7, LMP-1, and CCL3 promoter ...1 EBNA-2 binding sites at the CXCR7 gene locus in an LCL (GM12878) and in the Mutu III BL cell line using methods described previously ...of ...
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Marked, transient inhibition of expression of the Epstein-Barr virus latent membrane protein gene in Burkitt's lymphoma cell lines by electroporation.
... Because expression of EBNA-2 has been shown to affect the expression of LMP in several cell lines, the effect of electroporation on EBNA-2 mRNA levels in Raji cells was measured by North[r] ...
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