eGFP-fusion
Adenovirus Protein V Induces Redistribution of Nucleolin and B23 from Nucleolus to Cytoplasm
... the EGFP fusion products, the amino acid sequences of the minimal nucleolar targeting regions, and representative exam- ples of the different localization ...the fusion protein. Full-length protein V ...
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Intracellular Trafficking and Localization of the Pseudorabies Virus Us9 Type II Envelope Protein to Host and Viral Membranes
... jellyfish EGFP to the carboxy terminus of Us9 did not interfere with either the targeting of Us9 to the Golgi region or its ability to be incorporated into viral particles ...Us9-EGFP fusion protein ...
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Identification of the Lymantria disparNucleopolyhedrovirus Envelope Fusion Protein Provides Evidence for a Phylogenetic Division of the Baculoviridae
... envelope fusion protein of the budded form of Autographa californica multinucleocapsid NPV (AcMNPV) and its close ...(egfp) fusion using both immunofluorescence and fluorescence microscopy revealed ...
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Expression of Nonstructural Rotavirus Protein NSP4 Mimics Ca2+ Homeostasis Changes Induced by Rotavirus Infection in Cultured Cells
... (EGFP) fusion protein in mammalian HEK 293 cells elevates basal intracellular calcium levels more than twofold by a phospho- lipase C-independent mechanism ...
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Cryoelectron Microscopy of Protein IX-Modified Adenoviruses Suggests a New Position for the C Terminus of Protein IX
... (A) CsCl density gradient of Ad-IX-45-EGFP virions. Clarified 293A cell lysate was centrifuged through a discontinuous CsCl gradient and visualized under UV light. Green fluorescence was observed with Ad- ...
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A Large Nonconserved Region of the Tethering Protein Leashin Is Involved in Regulating the Position, Movement, and Function of Woronin Bodies in Aspergillus oryzae
... (EGFP) fusion protein. An N- and C-terminal fusion construct lacking the nonconserved middle region of AoLAH was sufficient for the tethering of Woronin bodies to the ...
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Expression of Magnaporthe grisea Avirulence Gene ACE1 Is Connected to the Initiation of Appressorium-Mediated Penetration
... display eGFP fluorescence in young (1 to 6 days) and old (7 to 15 days) mycelia grown in liquid (still/shake) or agar media (complete, minimal, or rice) nor in conidia produced from these ...weak eGFP ...
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Expression and cytosolic assembly of the S-layer fusion protein mSbsC-EGFP in eukaryotic cells
... mSbsC-EGFP fusion protein was recovered from the insoluble ...the fusion protein assembled in solution and yielded green fluorescent cylindric structures with regular symmetry comparable to that of ...
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Identification of human plasma cells with a lamprey monoclonal antibody
... CD38-EGFP fusion proteins before flow cytometric analysis for binding by VLRB MM3 or a conventional anti-CD38 Ab, we observed that VLRB MM3 recognized only the cells expressing the highest levels of ...
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Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells
... GFP fusion interference, it is most unlikely considering the fact the same method has been used successfully to reveal sub- cellular localization of MsrA in our cell lines ...MsrA-eGFP fusion protein ...
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Nontraditional Localization and Retention Signals Localize Human Cytomegalovirus pUL34 to the Nucleus
... the EGFP-UL34 fusion proteins were constructed and analyzed for their patterns of localization following deletion of amino acids 198 to ...from EGFP fusion plasmids express- ing UL34 amino ...
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Open Reading Frame E3-10.9K of Subspecies B1 Human Adenoviruses Encodes a Family of Late Orthologous Proteins That Vary in Their Predicted Structural Features and Subcellular Localization
... Subspecies B1 human adenoviruses (HAdV-B1s) are important causative agents of acute respiratory disease, but the molecular bases of their distinct pathobiology are still poorly understood. Marked differences in genetic ...
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A tripartite DNA-binding element, comprised of the nuclear localization signal and two AT-hook motifs, mediates the association of LEDGF/p75 with chromatin in vivo
... Lens epithelium-derived growth factor p75 (LEDGF/ p75) is a DNA-binding, transcriptional co-activator that participates in HIV-1 integration site targeting. Using complementary approaches, we determined the mechanisms of ...
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The ORF3 Protein of Hepatitis E Virus Delays Degradation of Activated Growth Factor Receptors by Interacting with CIN85 and Blocking Formation of the Cbl-CIN85 Complex
... ORF3- EGFP, mutant ORF3-EGFP fusion proteins, or EGFP only as a ...express EGFP (control) or the ORF3-EGFP or ⌬ D1-ORF3-EGFP fusion ...
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Activation and detection of HTLV I Tax specific CTLs by Epitope expressing Single Chain Trimers of MHC Class I in a rat model
... For the assessment of SCT expression, MOLT-4 cells trans- fected with various SCT expression vectors were stained with an anti-rat MHC-I antibody (BD Bioscience, San Jose, CA) for 30 min on ice, washed three times with ...
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Improved fusion protein expression of EGFP via the mutation of both Kozak and the initial ATG codon
... a fusion protein vector for many years ...by fusion protein ...toxin-EGFP fusion protein expression vector (pEGFP-BmKK2), western blotting analysis showed that there was almost no ...
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New mRNAs Are Preferentially Translated during Vesicular Stomatitis Virus Infection
... Figure 5 shows a model by which the translation program may be altered in infected cells, resulting in selective transla- tion of newly introduced mRNAs. Previous data have shown that inhibition of translation of host ...
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Subcellular Localization of Feline Immunodeficiency Virus Integrase and Mapping of Its Karyophilic Determinant
... In addition to the N terminus, we also analyzed the central catalytic and C-terminal domains of FIV integrase for their potential contribution to nuclear import. As discussed earlier, a putative bipartite NLS and a ...
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Dendritic cell defects in Wiskott-Aldrich Syndrome
... Whereas DCs that expressed EGFP alone showed diffuse fluorescence (Figure 4B,D ), EGFP- WASp clearly localized to podosomes (Figure 4F,H), Furthermore, expression of EGFP-WA[r] ...
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The Latency-Associated Transcript Gene Enhances Establishment of Herpes Simplex Virus Type 1 Latency in Rabbits
... structural EGFP gene [including a 3⬘ poly(A) signal] flanked by regions of LAT contained in dLAT2903 (LAT nucleotides ⫺798 to ⫹76 and 1667 to 1850) as described in Materials and ...of EGFP, one in each long ...
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