Ehrlichia ruminantium
Point Seroprevalence Survey of Ehrlichia ruminantium Infection in Small Ruminants in The Gambia
... Using the MAP1-B enzyme-linked immunosorbent assay, we tested 1,318 serum samples collected from sheep and goats at 28 sites in the five divisions of The Gambia to determine the Ehrlichia ruminantium ...
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Detection of Ehrlichia ruminantium infection in cattle in Cameroon
... Ehrlichia ruminantium (ER) is an obligate intracellular, Gram negative, pleomorphic, tick-transmitted bacte- rial pathogen recognized as an agricultural biothreat [1, ...
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Efficient high throughput molecular method to detect Ehrlichia ruminantium in ticks
... Ehrlichia ruminantium is an obligate intracellular bac- terium that causes heartwater, an infectious, virulent, transmissible but non-contagious disease of ruminants ...
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Multi locus sequence typing of Ehrlichia ruminantium strains from geographically diverse origins and collected in Amblyomma variegatum from Uganda
... bacterium Ehrlichia ruminantium is the causative agent of heartwater in ...E. ruminantium detected from its vector, Amblyomma variegatum, in heartwater-endemic areas in ...
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Longitudinal monitoring of Ehrlichia ruminantium infection in Gambian lambs and kids by pCS20 PCR and MAP1 B ELISA
... E. ruminantium- infected offspring survive/tolerate the ...E. ruminantium-positive by nested pCS20 PCR described above suggesting a carrier status which in time, resulted in mortality possibly due to stress ...
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Development of a Polyclonal Competitive Enzyme-Linked Immunosorbent Assay for Detection of Antibodies to Ehrlichia ruminantium
... at regular intervals following infection, aliquoted, and stored at ⫺20°C. Goats were kept tick free at the Centre for Tropical Veterinary Medicine under United Kingdom Home Office regulations for animal experimentation ...
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Detection by Two Enzyme-Linked Immunosorbent Assays of Antibodies to Ehrlichia ruminantium in Field Sera Collected from Sheep and Cattle in Ghana
... E. ruminantium PC-ELISA reactiv- ities from samples collected during longitudinal and point prevalence serosurveys carried out in Ghana between March 1994 and December ...E. ruminantium PC-ELISA (35) was ...
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Characterization of the Major Antigenic Protein 2 of Ehrlichia canis and Ehrlichia chaffeensis and Its Application for Serodiagnosis of Ehrlichiosis
... The map2 genes of E. canis and E. chaffeensis have signifi- cant homology to the map2 of Ehrlichia ruminantium (4) and the msp5 of Anaplasma marginale (17). The map2 of E. rumi- nantium and the msp5 of A. ...
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Ehrlichia chaffeensis Proliferation Begins with NtrY/NtrX and PutA/GlnA Upregulation and CtrA Degradation Induced by Proline and Glutamine Uptake
... or Ehrlichia) sennetsu and Neorickettsia (formerly Ehrlichia) risticii can utilize glutamine, but not glutamate, as an energy source (47, ...In Ehrlichia ruminantium-infected BA886 cells, a ...
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New species of Ehrlichia isolated from Rhipicephalus (Boophilus) microplus shows an ortholog of the E canis major immunogenic glycoprotein gp36 with a new sequence of tandem repeats
... Based on aa homology and genomic synteny analyses, it has been determined that the mucin-like protein of Ehrlichia ruminantium, gp36 of E. canis and gp47 of E. chaffeensis are orthologs [14]. Identity of ...
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Predominance of Ehrlichia ewingii in Missouri Dogs
... We highlight here the potential relationships between hu- man and canine ehrlichiosis. The finding that two of ten asymp- tomatic dogs were PCR positive for E. ewingii suggests that dogs might serve as a reservoir for E. ...
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Rumen methanogens and mitigation of methane emission by anti-methanogenic compounds and substances
... Methanobrevibacter ruminantium, Methanobrevibacter millerae, Methanobrevi- bacter olleyae, Methanomicrobium mobile, Methanoculleus olentangyi, and Methanosarcina barkeri ...
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Use of a specific immunogenic region on the Cowdria ruminantium MAP1 protein in a serological assay
... C. ruminantium isolates, ...to Ehrlichia bovis were obtained from ...to Ehrlichia chaffeensis was obtained from the Centers for Disease Control and Prevention, Atlanta, ...C. ruminantium ...
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Serological responses to Ehrlichia equi, Ehrlichia chaffeensis, and Borrelia burgdorferi in patients from New York State
... heat shock protein were second only to 41-kDa (flagellin) antibodies in both polyvalent and IgG immunoblots (Table 2). Ehrlichia species and B. burgdorferi serology reported by the Wadsworth Center and ERL. Table ...
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Validation of the Indirect MAP1-B Enzyme-Linked Immunosorbent Assay for Diagnosis of Experimental Cowdria ruminantium Infection in Small Ruminants
... C. ruminantium isolates by needle infection 1 month after vaccination with an attenuated Cowdria isolate originating from Senegal (17, ...C. ruminantium: Gardel (n 5 1), Senegal (n 5 16), Lutale (n 5 1), ...
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A cloned DNA probe identifies Cowdria ruminantium in Amblyomma variegatum ticks
... The pCR9 probe hybridized to midgut DNAs from 10 adult ticks allowed to feed as nymphs on infected goats but not from 10 adult ticks allowed to feed as nymphs on uninfected goats Fig.. S[r] ...
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Genotypic diversity of Streptococcus suis and the S. suis-like bacterium Streptococcus ruminantium in ruminants
... S. ruminantium described in this study only targets two genes, and thus, the possibility of the existence of more variations in the cps gene clusters cannot be ruled ...
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Intravascular persistence of Anaplasma platys, Ehrlichia chaffeensis, and Ehrlichia ewingii DNA in the blood of a dog and two family members
... and Ehrlichia gene targets not routinely assayed in our laboratory con- firmed the initial identification of pathogen DNA in all three family ...or Ehrlichia seroreactive at ...
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Ehrlichia chaffeensis, a new species associated with human ehrlichiosis
... Using universal primers that are conserved among eubacterial 16S rRNA genes, we were able to amplify, clone, and sequence 16S rDNA from all five currently recognized species of ehrlichia[r] ...
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Persistence of Ehrlichia phagocytophilaInfection in Two Age Groups of Lambs
... It should be mentioned that granulocytic Ehrlichia infection is found to persist in other species such as dogs (Egenvall et al. 2000) and red deer (Stuen et al. 2001), but not in horses and cattle (Madigan 1993, ...
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