eIF-2 (α-SP)
Internal translation initiation on poliovirus RNA: further characterization of La function in poliovirus translation in vitro.
... To further characterize La activity in translation and to identify other potential limiting factors, we assayed the ability of La protein as well as purified initiation factors, eIF-2, g[r] ...
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Cell-Type-Specific Growth Restriction of Vesicular Stomatitis Virus polR Mutants Is Linked to Defective Viral Polymerase Function
... and eIF-2 ␣ phosphorylation ...factor eIF-2B required for maintaining eIF-2 ␣ ...that eIF-2 ␣ phosphorylation is enhanced about threefold in polR- infected L-929 cells ...
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Herpes Simplex Virus 1 Infection Activates the Endoplasmic Reticulum Resident Kinase PERK and Mediates eIF-2α Dephosphorylation by the γ134.5 Protein
... PERK, eIF-2 ␣ and glycoprotein C ...␥ 2 gene), the result suggests that viral DNA replication is inhibited ...in eIF-2 ␣ phos- phorylation was observed in cells infected with R3616 but ...
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Inhibition of Protein Kinase R Activation and Upregulation of GADD34 Expression Play a Synergistic Role in Facilitating Coronavirus Replication by Maintaining De Novo Protein Synthesis in Virus-Infected Cells
... To confirm the effect of nsp2 on PKR-induced translation inhibition, a dose-dependent expression experiment was per- formed. The influenza A virus NS1 protein, known as a PKR antagonist, was used as a control. As shown ...
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Protein Kinase PKR Plays a Stimulus- and Virus-Dependent Role in Apoptotic Death and Virus Multiplication in Human Cells
... factor 2 (eIF-2 ␣ ). PKR catalyzes the phosphorylation of eIF-2 ␣ on serine 51, a modification that alters the translation pattern within cells and leads to an inhibition of protein ...
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Influenza virus regulates protein synthesis during infection by repressing autophosphorylation and activity of the cellular 68,000-Mr protein kinase.
... P68 was immunopurified from infected and, as a control, uninfected cell extracts, and kinase activity was measured with purified eIF-2 as an exogenous Downloaded from http://jvi.asm.org/[r] ...
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Adenovirus virus-associated RNA and translation control.
... Translational control by adenovirus: lack of virus-associated RNA, during adenovirus infection results in phosphorylation of initiation factor eIF-2 and inhibition of protein synthesis..[r] ...
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Protein Kinase PKR Mediates the Apoptosis Induction and Growth Restriction Phenotypes of C Protein-Deficient Measles Virus
... Western immunoblot analysis. Whole-cell extracts were prepared at the times postinfection indicated in the figures and legends in the presence of 1 mM phenylmethylsulfonyl fluoride and 1% (vol/vol) protease inhibitor ...
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AlaArg Motif in the Carboxyl Terminus of the γ134.5 Protein of Herpes Simplex Virus Type 1 Is Required for the Formation of a High-Molecular-Weight Complex That Dephosphorylates eIF-2α
... factor eIF-2 (eIF-2␣). Phosphorylation of eIF-2␣ increases its affinity for guanine nucleotide exchange factor eIF-2B, thus sequestering eIF-2B complex in an ...
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Antiapoptotic and Oncogenic Potentials of Hepatitis C Virus Are Linked to Interferon Resistance by Viral Repression of the PKR Protein Kinase
... of eIF-2␣ phosphorylation induced on- cogenic transformation (5, 6, 8, 21) and rendered cells refrac- tory to apoptosis induced by PKR agonists, including dsRNA (4, 18, ...of eIF-2␣ or ...
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EIB Group annual report 2008. Volume II: Financial report
... Euro Area Reference Note (EARN) benchmark issues accounted for the bulk (EUR 13bn, or 78%) of EUR issuance. New EARNs were launched in the 3-year and 7-year maturi- ties, each in EUR 3bn size. In addition, there were ...
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Important residue (G46) in erythroid spectrin tetramer formation
... Erythroid spectrin, a major skeleton protein in the red blood cell membrane, plays a crucial role in maintaining the unique shape, elasticity and deformability of erythrocytes [1-3]. The C-terminal region of the ...
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Intracellular soluble α synuclein oligomers reduce pyramidal cell excitability
... protein α-synuclein (αSyn) aggregates during Parkinson’s disease (PD) to form large proteinaceous amyloid plaques, the spread of which throughout the brain clinically defines the severity of the ...aggregation, ...
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Using Least Variance for Robust Extraction of Systolic Time Intervals
... 26 L1 Instruction Cache Warp Scheduler Warp Scheduler Dispatch Unit Dispatch Unit Register File 32 KB x 32-bit SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP SP [r] ...
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Inhibition of TANK Binding Kinase 1 by Herpes Simplex Virus 1 Facilitates Productive Infection
... tion. As illustrated in Fig. 4A, IRF3 was constitutively expressed in MEF cells, where it remained unphosphorylated in mock-infected cells (lanes 1 and 6). At 3 h postinfection, R3616 as well as H1001 stimulated IRF3 ...
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The structure of the AliC GH13 α-amylase from Alicyclobacillus sp. reveals the accommodation of starch branching points in the α-amylase family
... Alicyclobacillus sp. enzyme could accept -1,6 branches in at least the 2, +1 and +2 subsites, consistent with the three-dimensional structures with glucosyl moieties in the +1 and +2 subsites ...
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Inhibition of translation in cells infected with a poliovirus 2Apro mutant correlates with phosphorylation of the alpha subunit of eucaryotic initiation factor 2.
... To determine whether phosphorylation of eIF-2a is the mechanism of inhibition of protein synthesis in cells infected with the poliovirus mutant 2A-2, total cell proteins were resolved at[r] ...
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The IE180 Protein of Pseudorabies Virus Suppresses Phosphorylation of Translation Initiation Factor eIF2α
... FIG 4 PRV IE180-mediated suppression of eIF2 ␣ phosphorylation in ST cells. (A, B) ST cells were pretreated with thapsigargin (1 M for 1 h). Subsequently, cells were treated with cycloheximide (10 g/ l) prior to ...
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Han, Wei (2011): Iron and Palladium Catalyzed C-H Functionalization. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... Very recently, the Dong group described Pd II/0 -catalyzed ortho-arylations of O- phenylcarbamates with simple arenes and sodium persulfate (Scheme 46). 60 In contrast to the aforementioned anilide arylations, ...
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Molecular Determinants of the Regulation of Development and Metabolism by Neuronal eIF2α Phosphorylation in Caenorhabditis elegans
... the eif-2Ba(qd335);daf-28(sa191) mutant (see recessive eif-2Ba alleles in Table S2 in File ...and eif-2Ba(pk720)] to determine whether loss-of-function mutations in the genes contribute to the phe- ...
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