Electron transfer dissociation (ETD)
Middle-down electron capture dissociation and electron transfer dissociation for histone analysis
... As a test model, we used histones from MEL (murine erythroleukemia) cells treated with butyric acid or DMSO. After acid extraction, histone pellets were dried and fractionated using a reversed-phase C3 column. For ...
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Electron Transfer Dissociation of Peptides Generated by Microwave D-Cleavage Digestion of Proteins
... It is well-documented that highly charged peptide ions generated by ESI are well-suited for electron transfer dissociation (ETD), which produces c- and z-type fragment ions via gas-phase ion/ion ...
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Charge state dependent top-down characterisation using electron transfer dissociation
... using electron transfer dissociation Marko Rožman and Simon ...The dissociation of protein ions (5–30 kDa) as a function of charge state has been explored in order to suggest the optimal ...
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Electron Transfer Dissociation (ETD) Innovation with Integrity. Look Beneath the Surface. Ion Trap MS
... Bruker Daltonics – Leader in ETD Technology amaZon – Featuring ETD Since its commercial introduction in 2005, Bruker has been the leader in highly efficient and sensitive electron transfer ...
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Top-down analysis of 30-80 kDa proteins by electron transfer dissociation time-of-flight mass spectrometry
... on electron transfer dissociation-based TD MS characterization, for stressing the current possibilities offered by this experimen- tal set-up and further investigating the dependence of protein ...
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Clinical method evaluation of hemoglobin S and C identification by top-down selected reaction monitoring and electron transfer dissociation
... ETD, Electron transfer dissociation, Intact protein, Hemoglobin, Hemoglobin disorders, Hemoglobin variant, SRM, Selected reaction monitoring © The Author(s) ...
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Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides
... Baker, Electron transfer dissociation of native peptides facilitates enhanced identification of urinary peptides, International Journal of Mass Spectrometry (2015), http://dx.doi.org/10.[r] ...
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Confident and sensitive phosphoproteomics using combinations of collision induced dissociation and electron transfer dissociation
... Of the very high confidence set (0.01 PEP threshold for both) of 12,618 CID/ETD pairs identified in DDNL experiments, 10,902 (86.4%) phosphorylation site assignments (rank 1 assignment f[r] ...
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Peptide and Protein Quantification Using iTRAQ with Electron Transfer Dissociation
... For the evaluation of protein quantification, only scans with sum total reporter ion intensities of 1000 or greater were included for ion trap measurements, and 100,000 for spectra acquired in the orbitrap. The ob- ...
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Investigation of the mechanism of electron capture and electron transfer dissociation of peptides with a covalently attached free radical hydrogen atom scavenger
... for transfer of the fluoranthene anion were optimized using the auto-tune method and the signal level of the fluoranthene anion at m/z 202 was ...after electron transfer for ETcaD experiments was ...
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Analysis of Arginine and Lysine Methylation Utilizing Peptide Separations at Neutral pH and Electron Transfer Dissociation Mass Spectrometry
... nine is converted by the cell to heavy SAM, which serves as a universal methyl-group donor in methyl transfer reactions including arginine methylation. The SFPQ/NONO protein complex was purified from these heavy ...
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Ion Mobility-Resolved Collision-Induced Dissociation and Electron Transfer Dissociation of N-Glycopeptides: Gathering Orthogonal Connectivity Information from a Single Mass- Selected Precursor Ion Population
... glycoproteomic inquiry. Here, a previously developed method of multiplexing CID and ETD is applied to the study of glycopeptides for the first time. Integration of the two dissociation methods was accomplished ...
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High-Energy Electron Transfer Dissociation (HE-ETD) Using Alkali Metal Targets for Sequence Analysis of Post-Translational Peptides
... the dissociation mechanism of the charge-reduced ions formed from electron-transfer is similar in spite of significantly dif- ferent collision energies available in ...
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Enhanced Peptide Identification by Electron Transfer Dissociation Using an Improved Mascot Percolator
... Direct comparison between search methods has been made by calculating the percentage increase in PSMs identified by Mascot Percolator compared with Mascot and OMSSA at each m / z and cha[r] ...
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Applications of ion mobility spectrometry, collision-induced dissociation and electron activated dissociation tandem mass spectrometry to structural analysis of proteins, glycoproteins and glycans
... collision-induced dissociation (CID), and electron activated dissociation (ExD) to structural analysis of various biomolecules, we applied different IMS and LC methods for separation and purification ...
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Studies in Recombination and Dissociation Reactions for Collisional Energy Transfer and Electron Transfer of Nanocrystals and Dye Molecules
... energy transfer for unimolecular dissociation/bimolecular recombination reactions is ...energy transfer, and the most realistic model, a model with a near-singularity, are fitted to the trajectory ...
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Ultrafast Electron Transfer from Quantum Dots: Towards Multi-exciton Dissociation
... Ultrafast Electron Transfer from Quantum Dots: towards Multi-Exciton Dissociation.. - -.[r] ...
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Electron impact induced dissociation of N2H+ into NH+
... 1. Introduction Collisions between electrons and molecular ions lead to reactions which differ drastically with increasing energy. At the lowest energy range (from 0 eV), only the exothermic dissociative recombination ...
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Near-threshold electron impact dissociation of molecular hydrogen
... Chapter 4: Electron-H 2 calculations over a range o f bond lengths 4.4 Effect of increasing the number of target states Earlier coupled state calculations (Fliflet & McKoy 1980, Baluja et al. 1985, Schneider & ...
152
Interplay of Proton Transfer, Electron Transfer and Proton Coupled Electron Transfer in Transition Metal Mediated Nitrogen Fixation
... The final chapter of this thesis discusses efforts to increase the accuracy of DFT predicted bond dissociation free-energies (BDFE E-H ) of transition metal bound E–H bonds. The importance of BDFE E-H predictions ...
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