embryoid body
Phenotype characterization of embryoid body structures generated by a crystal comet effect tail in an intercellular cancer collision scenario
... generate embryoid body structures in a space-time ...which embryoid bodies were identified to determine the phenotype char- acterization that serves as a guideline for easy ...of embryoid ...
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Intercellular cancer collisions generate an ejected crystal comet tail effect with fractal interface embryoid body reassembly transformation
... Molecular biologic crystals, represented by triangular chiral hexagons derived from collision attraction events against col- lagen type I fibrils, emerge at microscopic and macroscopic levels. This phase is full of ...
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Modelling human embryoid body cell adhesion to a combinatorial library of polymer surfaces
... for experimentally measured properties in modelling hEB adhesion on polymer surfaces, we additionally generated machine-learning models of surface roughness that also employed calculated[r] ...
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Nuclear reprogramming with a non-integrating human RNA virus
... ATU: additional transcription unit; DMEM: Dulbecco ’ s modified Eagle ’ s medium; EB: embryoid body; FCS: fetal calf serum; FITC: fluorescein isothiocyanate; GFP: green fluorescent prote[r] ...
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Conjoint propagation and differentiation of human embryonic stem cells to cardiomyocytes in a defined microcarrier spinner culture
... Pluripotent hESCs have been generally differentiated in two different platforms either on tissue culture plates [16-20] or embryoid body (EB) cultures [21,22]. The sus- pended EB cultures have the potential ...
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Attenuation of teratoma formation by p27 overexpression in induced pluripotent stem cells
... Results: Overexpression of p27 inhibited cell division of miPSCs, and that inhibition was dependent on the expression level of p27. p27 overexpressing miPSCs had pluripotency characteristics but lost stemness earlier ...
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Key genes and integrated modules in hematopoietic differentiation of human embryonic stem cells: a comprehensive bioinformatic analysis
... EB: Embryoid body; EHT: Endothelial-to-hematopoietic transition; FACS: Fluorescence-activated cell sorting; FDR: False discovery rate; FITC: Fluorescein isothiocyanate; GO: Gene Ontology; HE: Hemogenic ...
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Reprogramming of blood cells into induced pluripotent stem cells as a new cell source for cartilage repair
... Generating integration-free hiPSCs and hiPSC culture The human episomal vectors pEV SFFV-OS (OCT4-2a- SOX2), pEV SFFV-MK (MYC-2a-KLF4), and pEV SFFV-B (BCL-XL) were kindly donated by Dr. Xiao-Bing Zhang. PBMNCs were ...
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Physical developmental cues for the maturation of human pluripotent stem cell-derived cardiomyocytes
... ARVD/C: Arrhythmogenic right ventricular dysplasia/cardiomyopathy; BDM: 2,3-butanedione monoxime; CM: Cardiomyocyte; Cx43: Connexin 43; EB: Embryoid body; ECM: Extracellular matrix; EHT:[r] ...
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Progress on stem cell research towards the treatment of Parkinson's disease
... 6-hydroxydopamine, DA, dopaminergic; EB, embryoid body; ES, embryonic stem; FGF, fi broblast growth factor; GDNF, glial cell line-derived neurotrophic factor; GIRK2, G protein-coupled [r] ...
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Human-induced pluripotent stem cells generated from intervertebral disc cells improve neurologic functions in spinal cord injury
... EB: Embryoid body; ESC: Embryonic stem cell; FBS: Fetal bovine serum; FITC: Fluorescein isothiocyanate; GFAP: Glial fibrillary-associated protein; H&E: Hematoxylin and eosin; hESC: Human embryonic stem ...
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Loss of non-coding RNA expression from the DLK1-DIO3 imprinted locus correlates with reduced neural differentiation potential in human embryonic stem cell lines
... Figure 3 MEG3-OFF human embryonic stem cells (hESCs) displayed different transcriptome profiles, particularly in genes related to neural lineage. (A) In total, 114 genes displayed significant differential expression ...
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Defining stemness of human embryonic stem cells: a systems biology approach
... Abbreviations Cell types and related EB: embryoid body ECC: embryonal carcinoma cell EGC: embryonic germ cell epiSC: epiblast stem cell ESC: embryonic stem cell hESC: human ESC mESC: mou[r] ...
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Human-induced pluripotent stem cell-derived macrophages and their immunological function in response to tuberculosis infection
... Methods: In the present study, macrophages derived from hiPS were established via embryoid body (EB) formation by using feeder-free culture conditions, and the human monocyte cell line THP-1 (THP-1-M φ ) ...
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Motor neuron derivation from human embryonic and induced pluripotent stem cells: experimental approaches and clinical perspectives
... ALS: Amyotrophic lateral sclerosis; BDNF: Brain-derived neurotrophic factor; BMP: Bone morphogen protein; ChAT: Choline acetyltransferase; CNS: Central nervous system; EB: Embryoid body;[r] ...
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Human endogenous retrovirus K (HML 2) RNA and protein expression is a marker for human embryonic and induced pluripotent stem cells
... To verify this with a second method and cell line, we performed real-time RT-PCR analysis using differentiating H9 ESCs [19,20]. This analysis showed an even more dramatic reduction of HERV-K(HML-2) RNA expression during ...
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Comparative analysis of ABCG2+ and ABCG2- retinoblastoma cells in three-dimensional culture
... Embryoid Body Assay/In vitro tumor assay: Although both the WERI-Rb27 cells and Y79 cells normally grow in suspension, each cell line was subjected to the same culture conditions used for embryoid ...
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Effect of Different Concentrations of Forskolin Along with Mature Granulosa Cell Co-Culturing on Mouse Embryonic Stem Cell Differentiation into Germ-Like Cells
... cAMP, cyclic adenosine monophosphate; EB, embryoid body; ES, embryonic stem; GC, granulosa cells; RA, retinoic acid Effect of Different Concentrations of Forskolin Along with [r] ...
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Soluble factors secreted by differentiating embryonic stem cells stimulate exogenous cell proliferation and migration
... of embryoid body conditioned medium EBs differentiated for 4, 7, or 10 days were collected (as described above), rinsed three times with phosphate- buffered saline (PBS, pH ...
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Human iPSC-derived MSCs (iMSCs) from aged individuals acquire a rejuvenation signature
... aMSC: Adult MSC; CD: Cluster of differentiation; DAVID: Database for Annotation, Visualization and Integrated Discovery; EB: Embryoid body; ESCs: Embryonic stem cells; FACS: Fluorescence[r] ...
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