Embryonic-like stem cell
Efficient differentiation of human embryonic stem cells to arterial and venous endothelial cells under feeder- and serum-free conditions
... apoptotic cell death using AnnexinV-Propidium iodide staining revealed the occur- rence of cellular apoptosis in a dose-dependent manner with the optimal concentration around 5–10 ng/ml VEGF (Additional file 1: ...
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Reinterpretation of evidence advanced for neo- oogenesis in mammals, in terms of a finite oocyte reserve
... “putative stem cells ” generated adherent oocyte-like cells, 20-95 μ m in diameter, with ZP-like, germinal vesicle-like and polar body-like structures that were ascribed to an oocyte ...
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Making gametes from alternate sources of stem cells: past, present and future
... tent stem cells termed very small embryonic-like stem cells (VSELs) in all adult organs including testis and ovary, which can also be differentiated into gametes in ...hematopoietic ...
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Generation of functional salivary gland tissue from human submandibular gland stem/progenitor cells
... gland stem cells, Pringle and colleagues mixed Matrigel and collagen to drive differentiation of cultured spheres into branching and lobular structures ...gland stem/progenitor cells and drive its ...
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Targeted DamID reveals differential binding of mammalian pluripotency factors
... enable cell type-specific expression in mammalian systems, generating an inducible system (mammalian TaDa or MaTaDa) to identify genome-wide protein/DNA interactions in 100 to 1000 times fewer cells than ...
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Pluripotent Human embryonic stem cell derived neural lineages for in vitro modelling of enterovirus 71 infection and therapy
... the cell via- bility of SH-SY5Y cells was quite resistant to EV71 infec- tion ...the cell culture supernatant with qRT-PCR, even though this was still the lowest amongst the four different cell types ...
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Defining stemness of human embryonic stem cells: a systems biology approach
... proteins like OCT4, which is a key transcription factor for hESC ...hubs like OCT4 (which are vulnerable nodes and can disturb the entire system if they are targeted), but also there is redundancy to make ...
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The miR-290-295 cluster as multi-faceted players in mouse embryonic stem cells
... chromatin conformations, which make it possible to switch to any type of cells [68]. Epigenetic modification plays an important role in the proliferation and differen- tiation of ESCs, while the miR-290-295 cluster is ...
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The Epigenomics of Embryonic Stem Cell Differentiation
... Among, HATs, Tip60/p400 has been identified as a critical requirement for ESC self-renewal and pluripotency [63]. Tip60/p400 contains both H2A.Z deposition and HAT activities combined. The mam- malian p400 contains ...
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Systems biology discoveries using non-human primate pluripotent stem and germ cells: novel gene and genomic imprinting interactions as well as unique expression patterns
... germ cell lineages, specifi cally in vitro-derived PGCs (invPGCs) ...germ cell gene expression for CXCR4, NANOS1, NANOS2, NANOS3, VASA, PIWIL1 and TEKT1 upon EB formation with retinoic acid for 28 ...mouse ...
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Dynamics associated with spontaneous differentiation of ovarian stem cells in vitro
... ovarian stem cells are relatively few in number because of scarcity of ovarian tissue for re- ...these stem cells gener- ated functional oocytes in vitro irrespective of age and condition (menopausal and ...
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Canine mammary tumors contain cancer stem like cells and form spheroids with an embryonic stem cell signature
... Hydro Cell plates for 21 days (D-G): stained against Sox2 (A,D); Oct-4 (B,E); Ki-67 (C,F); and Asp175 (Cleaved Caspase-3), ...most cell nuclei were labeled with the Sox2 antibody ...
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Directed differentiation of rhesus monkey ES cells into pancreatic cell phenotypes
... Embryonic stem cells (ES) can self-replicate and differentiate into all cell types including insulin- producing, beta-like cells and could, therefore, be used to treat diabetes ...of ...
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Haploid embryonic stem cells serve as a new tool for mammalian genetic study
... ES cell differentiation, chimeric embryos were produced from ahES cells carrying green fluorescence protein and the DNA contents of green fluorescence protein-labeled cells were ...on embryonic day ...loid ...
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In vitro myelin formation using embryonic stem cells
... axons like a snake, forming a perfect corkscrew-like structure, as was previously suggested in the variants of the serpent model, because the process was anchored on the axon before wrapping, as was also ...
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Induced pluripotent stem cell-derived cardiomyocytes for cardiovascular disease modeling and drug screening
... Pluripotent stem cells have also been explored as a promising cell source for cardiac cell ...as cell immunogen- icity, survival, and electrophysiological integration, remain to be addressed ...
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Serum starvation-induced cell cycle synchronization stimulated mouse rDNA transcription reactivation during somatic cell reprogramming into iPSCs
... duced cell cycle synchronization, resulting in a notable enrichment of the G0/G1 phase in fibroblasts when compared with normal control (Additional file 2: Figure ...These cell cycle profiles were in ...
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Vitamin D3 stimulates embryonic stem cells but inhibits migration and growth of ovarian cancer and teratocarcinoma cell lines
... (Lineage Cell Depletion kit, Miltenyi Biotec), magnetic labeling of retrieved total nucleated cells was performed, and the lineage-negative (Lin − ) cells were isolated by depletion of mature hematopoietic cells ...
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Improved hematopoietic differentiation of human pluripotent stem cells via estrogen receptor signaling pathway
... for stem cell factor, is one of EGR1 direct target genes whose transcription is directly regulated by EGR1 in the uterus (data not ...important cell surface marker sued to iden- tify hematopoietic ...
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A versatile genetic tool: haploid cells
... haploid cell lines, basic studies including DNA repair, drug toxicity, X-chromosome inactivation, cell differentiation, and human clinical diseases have been investigated separately [3–5, 7, 8, 23, 29, ...
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