Empty Capsids
Dissecting the roles of VP0 cleavage and RNA packaging in picornavirus capsid stabilization: the structure of empty capsids of foot-and-mouth disease virus.
... We thank D. Goodrich for data collection trips as disease security officer and the staff of the Synchrotron Radiation Source, Daresbury FIG. 6. Differences in the putative active sites of VP0 cleavage may account for the ...
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Structural Characterization of H-1 Parvovirus: Comparison of Infectious Virions to Empty Capsids
... and empty capsid suggests that the bound genome reinforces the stability of the particle, especially at the 2-fold symmetry axis ...that empty capsids are assembled prior to genome packaging for ...
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Processing of the VP1/2A Junction Is Not Necessary for Production of Foot-and-Mouth Disease Virus Empty Capsids and Infectious Viruses: Characterization of “Self-Tagged” Particles
... capsid precursor of FMDV includes the 2A protein; therefore, it is called P1-2A. 3C pro is responsible for processing of the FMDV P1-2A precursor into VP0, VP3, and VP1 plus 2A. There is evi- dence that cleavage of the ...
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Poliovirus empty capsid morphogenesis: evidence for conformational differences between self- and extract-assembled empty capsids.
... We found that incubation of partially purified 14S particles with mock-infected cell extracts resulted in the assembly of empty capsids, some about 20% of which focused at pH 6.8 17 and [r] ...
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Polypeptide Composition of Poliovirions, Naturally Occurring Empty Capsids, and 14S Precursor Particles
... Fraction of total radioactivity present in the polypeptides of type 1 Mahoney virions, empty capsids, and 14S particles Viral polypeptides Particle NCVP 1-2 NCVP 6 VP 1 VP 2 VP 3 VP 4 Vi[r] ...
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Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins.
... Since small amounts of empty capsids of human parvovirus B19 1,000 to 2,000 per cell are formed spontaneously from their components in Chinese hamster ovary cells 12, the possibility tha[r] ...
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Polypeptide composition of rotavirus empty capsids and their possible use as a subunit vaccine.
... Labeled polypeptides detected by autoradiography in extracellular viral SS particles and EC grown in the presence 0 or absence 0 of trypsin T and presence U or absence L of trypsin inhib[r] ...
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Differences between poliovirus empty capsids formed in vivo and those formed in vitro: a role for the morphopoietic factor.
... PHILLIPS* Department ofMicrobiology, School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 15261 Empty capsid species formed from the self- and extract-mediated assembly[r] ...
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Characterization of post-translational products of herpes simplex virus gene 35 proteins binding to the surfaces of full capsids but not empty capsids.
... This monoclonal antibody reacted with six bands ICP35a to 35f formed by ICPs contained in either HEp-2 or Vero cell lysates electrophoretically separated in denaturing gels and transferr[r] ...
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Viral RNA modulates the acid sensitivity of foot-and-mouth disease virus capsids.
... and empty capsids were produced by infecting BHK-21 cell ...natural empty capsids were pooled separately and stored at 2 80 8 C between ...
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Phosphorylation of Simian Virus 40 Proteins in a Cell-Free System
... After phosphorylation in vitro of either empty capsids or infectious virus by beef heart kinase, the virus particles were banded in KBr and precipitated with 10% trichloroacetic acid.. S[r] ...
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In Vitro Assembly of an Empty Picornavirus Capsid follows a Dodecahedral Path
... To further understand the structural basis of assembly, we ex- amined the pentamer by using negative-stain TEM, and we ob- tained a 3-D model at 30-Å resolution (Fig. 5). The 2-D class averages of particles, with no ...
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Modest truncation of the major capsid protein abrogates B19 parvovirus capsid formation.
... virus capsids in a baculovirus system does not require the presence of VP1 ...forms capsids with low efficiency and distorted morphology ...VP2-only capsids do not elicit a neutralizing antibody ...
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A Conformational Change in the Adeno-Associated Virus Type 2 Capsid Leads to the Exposure of Hidden VP1 N Termini
... VP2 capsids had significantly less variability than the reconstruction of ⌬ VP1 ...wild-type empty capsids together with reconstructions of mutant empty capsids (either ⌬ VP1 or ⌬ VP2) ...
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Adenovirus L1 52- and 55-kilodalton proteins are present within assembling virions and colocalize with nuclear structures distinct from replication centers.
... Third, the 52/55-kDa proteins were not detected in mature virions, but they are present in all putative virion assembly structures: empty capsids, intermediate particles, and young virio[r] ...
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RNA binding properties of poliovirus subviral particles.
... pentamers, empty capsids, and provirions (54, ...the empty capsid, both in vivo and in vitro (4, 33, 54). The empty-capsid particles sediment at 75S and contain no RNA ...the empty ...
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An assembly defect as a result of an attenuating mutation in the capsid proteins of the poliovirus type 3 vaccine strain.
... Alternatively, if lower-order structures such as protomers and/or pentamers were degraded at a greater rate than empty capsids and virions, the observed difference in stability of SV3/L [r] ...
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Formation of empty B19 parvovirus capsids by the truncated minor capsid protein.
... 4690 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest We previously reported that empty capsids of B19 parvovirus were formed by the major capsid protein VP2 alone expres[r] ...
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Unique region of the minor capsid protein of human parvovirus B19 is exposed on the virion surface
... Capsids of the B19 parvovirus are composed of major (VP2; 58 kD) and minor (VP1; 83 kD) structural proteins. These proteins are identical except for a unique 226 amino acid region at the amino terminus of VP1. ...
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A functional role for the conserved protonatable hairpins in the 5' untranslated region of turnip yellow mosaic virus RNA.
... The 5 * untranslated region (UTR) of the RNA of several tymoviruses contains conserved hairpins with protonatable internal loops, consisting of C-C and C-A mismatches (K. Hellendoorn, P. J. A. Michiels, R. Buitenhuis, ...
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