empty vector
RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer
... Abbreviations: EV, empty vector; KD, knockdown; NSCLC, non-small-cell lung cancer; RKIP, Raf kinase inhibitor protein; A-EV, A549 cells transfected with empty vector; A/RKIP-KD, A549 c[r] ...
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Hypoxia-inducible factor-mediated induction of WISP-2 contributes to attenuated progression of breast cancer
... an empty (e) vector, or a HiF-1α (H1) or HiF-2α (H2) overexpression ...respective empty vector controls (A and B), or were first normalized to the empty vector control values and ...
11
Original Article Guanylate cyclase-C signaling pathway regulates intestinal inflammatory injury and epithelial barrier function in Caco-2 cells
... amine 2000 and plasmid DNA were diluted in 375 μl of Opti-MEM medium (Gibco, USA) sepa- rately and incubated at room temperature for 10 min after mixing. The lipofectamine-DNA complex was added to cells and incubated at ...
11
Phosphorylation and inactivation of PTEN at residues Ser380/ Thr382/383 induced by Helicobacter pylori promotes gastric epithelial cell survival through PI3K/Akt pathway
... Immunoblots of phosphatase and tensin homolog PTEN, phosphorylated p-PTEN, Akt, p-Akt, Bad, p-Bad and β-actin from gastric epithelial cells GES-1 stably expressing an empty vector Empty,[r] ...
11
Epstein-Barr Virus Latent Membrane Protein 1 Represses DNA Repair through the PI3K/Akt/FOXO3a Pathway in Human Epithelial Cells
... LMP1 represses DNA repair, and the CTAR1 domain is required. We demonstrated previously that LMP1 could re- press DNA repair in a p53-independent manner, and the CTAR1 domain was required for this repression (40). To ...
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Efficient TRAIL Gene Delivery Using Nucleofection Based Method in Human Adiposed Derived Mesenchymal Stromal Cells
... the empty vector (EV; RFP+) and target vector (TRAIL; GFP+) compared to lipofection with optimal cellular viability noticed in all transfection ...target vector (TRAIL; GFP+) reporter ...
6
Eosinophilic esophagitis–linked calpain 14 is an IL-13–induced protease that mediates esophageal epithelial barrier impairment
... CAPN14 overexpression disrupts DSG1 expression. We next investigated the effect of CAPN14 overex- pression on junctional protein integrity. We first interrogated junctional protein expression by Western blot analysis of ...
12
Original Article The overexpression of uPA promotes the proliferation and fibrinolytic activity of human umbilical vein endothelial cells
... adenovirus vector carried the enhanced green fluorescent protein (EGFP) gene, which could be seen under a fluorescence microscope after they were ...the empty vector group showed green fluorescence, ...
8
The SAMHD1-mediated block of LINE-1 retroelements is regulated by phosphorylation
... expression vector or empty vector were transfected into 293T cells at a molecular ratio of 3:1 using calcium ...with empty vector or the indicated SAMHD1 expression plasmid using ...
17
Prolonged Activity of the Pestiviral RNase Erns as an Interferon Antagonist after Uptake by Clathrin-Mediated Endocytosis
... Again, cells incubated with supernatant concentrated from cells expressing the empty vector only slightly reduced the expression of the Mx protein in response to polyI·C, and this was po[r] ...
9
Hyperlipidaemia alone and in combination with acidosis can increase the incidence and severity of statin induced myotoxicity
... MTT assay of undifferentiated and differentiated C2C12 cells overexpressing mLPL, hLPL, and control cells transfected with empty vector following treatment with simvastatin either loaded[r] ...
39
Japanese Encephalitis Virus Replication and Inhibitory Effect of shRNA in Mice
... Interestingly, inhibitory effect on JEV replication in the mice was observed in the injection of not only pJRi but also the empty vector pSilencer (Figure 4, Figure 5). There is a possibility that injection ...
9
Porcine Reproductive and Respiratory Syndrome Virus Nsp1β Inhibits Interferon-Activated JAK/STAT Signal Transduction by Inducing Karyopherin-α1 Degradation
... as expected, and that its density was 4.18-fold greater than in the cells transfected with empty vector (Fig. 4A). As Laemmli sample buffer was used as the elusion buffer in IP, the heavy and light chains ...
10
Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis
... The four plasmids pHT01 (Pgrac01, no bgaB gene), pHT01-bgaB (Pgrac01-bgaB, inducible), pHCMC02- bgaB (PlepA-bgaB, inducer-free) and pHT1655 (inducer- free) were transformed into the B. subtilis strain 1012. The strains ...
10
Expression of ID4 protein in breast cancer cells induces reprogramming of tumour associated macrophages
... expression vector containing a hemagglutinin (HA)-tagged ID4 coding sequence [28] or control empty vector was transfected in cancer cells using Li- pofectamine 2000 reagent (Thermo Fisher Scientific, ...
15
miR-1/206 downregulates splicing factor Srsf9 to promote C2C12 differentiation
... The inability to downregulate Srsf9 expression during myogenesis results in impaired differentiation Finally, we investigated whether the downregulation of Srsf9 expression plays an important role in controlling the ...
12
Impaired osteoblastic differentiation, reduced bone formation, and severe osteoporosis in noggin overexpressing mice
... Viral vector (pLXSN; Clontech Laboratories ...or vector containing either the murine noggin gene or the constitutively active ALK-3 (ca-ALK-3) gene were trans- fected into Phoenix cells (Phoenix cells ...
12
A Particle-Associated Glycoprotein Signal Peptide Essential for Virus Maturation and Infectivity
... Gag/Pol-expressing vector pHIT60 and the MuLV retroviral vector pczCFG2 fEGN together with different FV Env expression constructs as ...Gag/Pol-expressing vector plasmid pMH62 into 293T cells and ...
10
Original Article Construction of HSP27-expressing lentiviral vector and interaction with contraction-associated proteins in the infection model with cells
... lentiviral vector and its effect on contraction-related proteins in the infection model in rat detrusor smooth muscle cells ...lentiviral vector expressing phosphory- lated HSP27, which was packaged and ...
8
Development, characterization, and skin delivery studies of related ultradeformable vesicles: transfersomes, ethosomes, and transethosomes
... Abbreviations: e caf, caffeine-loaded ethosomes; empty e, empty ethosomes; empty Te Naco, empty sodium cholate transethosomes; empty Te span, empty span 20 transethosomes; empty T Naco[r] ...
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