Endo H
Enzyme Digests Eliminate Nonfunctional Env from HIV-1 Particle Surfaces, Leaving Native Env Trimers Intact and Viral Infectivity Unaffected
... In Fig. 4A, lane 1, gp160ER staining is relatively prominent compared to the gp120 band that likely derives from native trimers. This suggests that gp160ER constitutes a surprisingly dominant fraction of total VLP Env ...
15
Maturation of Human Herpesvirus 6A Glycoprotein O Requires Coexpression of Glycoprotein H and Glycoprotein L
... FIG 2 Cleavage of gO during its maturation process. (A) 293T cells (top) and HEK293SGnTI ⫺ cells (bottom) were transfected with a plasmid expressing hemagglutinin (HA)-tagged gO (AgOHA), either alone (lanes 2 to 4) or in ...
5
Structures of the Oligosaccharides of the Glycoprotein Coded by Early Region E3 of Adenovirus 2
... In our analyses of the oligosaccharides released by endo H, we found that the pulselabeled sample, which should contain newly synthesized and mature E3-gp25K, gave rise to equal amounts [r] ...
10
Rous sarcoma virus glycoproteins contain hybrid-type oligosaccharides.
... A fraction of the medium-size glycopeptides fractions 61 to 70 was resistant to endo-H, but the majority of radiolabel was converted to a major peak eluting in the same position as the u[r] ...
5
Endo-beta-N-acetylglucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC.
... Endo H treatment of anti-gAB immunoprecipitated proteins from HSV-1-infected MRC-5 cells incubated in the absence or presence of 10-6 or 10-7 M monensin.. HSV-1-infected cells were label[r] ...
8
Defective transport of Sindbis virus glycoproteins in End4 mutant Chinese hamster ovary cells.
... Sensitivity of oligosaccharide groups of membrane glycoproteins to the enzyme endo H was used as an assay for measuring the extent of transport and processing of Sindbis virus proteins i[r] ...
8
Characterization of Two New Structural Glycoproteins, GP3 and GP4, of Equine Arteritis Virus
... endoglycosidase H (endo H) and N-glycosidase F (PNGase F) digestion were ...of endo H buffer (50 mM sodium acetate [pH ...without endo H (New England Biolabs) or PNGase F ...
12
Luteinizing hormone and androstendione are independent predictors of ovulation after laparoscopic ovarian drilling: a retrospective cohort study
... Hayashi H, Ezaki K, Endo H, Urashima M: Preoperative luteinizing hormone levels predict the ovulatory response to laparo- scopic ovarian drilling in patients with clomiphene citrate- re[r] ...
7
Formation and rearrangement of disulfide bonds during maturation of the Sindbis virus E1 glycoprotein.
... In this respect, the processing of El to an endo H-resistant form in BFA-treated cells is similar to that reported for vesicular stomatitis virus G protein.. After the extended 5-h chase[r] ...
8
Identification and characterization of pseudorabies virus glycoprotein H.
... These results show that i gHPrV, as detected by our antisera in purified virion preparations, does indeed constitute a glycosylated protein; ii approximately S kDa of endo H-sensitive ca[r] ...
8
Primate cytomegalovirus glycoproteins: lectin-binding properties and sensitivities to glycosidases.
... B Nonlabeled Colburn virions were treated with endo H or endo F, as indicated, subjected to SDS-PAGE, electrotransferred onto nitrocellulose, and probed with 125I-ConA lanes marked ConA [r] ...
11
Structural plasticity of the Semliki Forest virus glycome upon interspecies transmission
... Alphaviral glycosylation is an important determinant of cellular tropism and virulence, 22−25 and the composition of alphavirion glycans derived from mammalian cell lines has been the subject of extensive study. 26−36 ...
12
Characterization of a herpes simplex virus type 2 75,000-molecular-weight glycoprotein antigenically related to herpes simplex virus type 1 glycoprotein C.
... Effect of endo H on newly synthesized and immunoprecipitation with anti-gF-2 111188 processed forms of gF-2 immunoprecipitated monoclonal antibody followed by preparative anti-gF-2 III18[r] ...
10
Lipopolysaccharide-induced inflammation or unilateral ureteral obstruction yielded multiple types of glycosylated Lipocalin 2
... LCN2, Endo H was partially effective in releasing N-glycan from the 22-kDa LCN2 in the liver and the ureter, whereas this protein minimally cleaved the carbo- hydrates on LCN2 from the sera, lungs, and ...
10
Multi-walled carbon nanotubes induce human microvascular endothelial cellular effects in an alveolar-capillary co-culture with small airway epithelial cells
... To determine the levels of inflammatory mediators in co-culture, VEGFA and two other secreted proteins known to have a role in the inflammatory process, soluble intracel- lular adhesion molecular 1 (sICAM-1) and soluble ...
14
5 endo,6 endo Bis(acetoxymethyl)bicyclo[2 2 1]hept 2 ene
... The title compound, (I), was prepared by the reaction of 5- endo,6-endo-bis(hydroxymethyl)bicyclo[2.2.1]hept-2-ene and acetic acid anhydride (Bailey & Lawson, 1955). The crystal structure determination ...
6
Ebolavirus Glycoprotein GP Masks both Its Own Epitopes and the Presence of Cellular Surface Proteins
... intracellular domain. Using antibodies against the extracellular part of the protein and GFP fluorescence, we were able to efficiently monitor its surface expression through visualization of both extracellular and ...
6
Angiopoietin-1 Protects Heart against Ischemia/Reperfusion Injury through VE-Cadherin Dephosphorylation and Myocardiac Integrin-β1/ERK/Caspase-9 Phosphorylation Cascade
... through endo thelial monolayers as a measure of permeability, we found that cAng-1 sig- nificantly reduced endothelial leakage by H/Reoxy in a dose-dependent man- ner (P < ...
12
Copper mediated cyclization of 1 substituted enamides, dienamides and trienamides : regiochemistry, indigoid formation and methyl migration aromatization
... 3. Radical polar crossover reaction: (a) Bashir, N.; Patro, B.; Murphy, J. A. Advances in Free Radical Chemistry; Zard, S. Z., Ed.; Jai Press: Stamford, CT, 1999; Vol. 2. 123. Copper mediated: (b) Davies, D. T.; Kapur, ...
6
Trivial source bimodule rings for blocks and p-permutation equivalences
... G, H such that the block algebras OGb, OHc are Morita equivalent via an OGb-OHc-bimodule M with endo-permutation source V , then by a result of Puig [24], b and c have isomorphic defect groups, and there is ...
35