Envelope protein domain III
Role of Zika Virus Envelope Protein Domain III as a Target of Human Neutralizing Antibodies
... 18–20). Protein binding-based assays performed to distinguish DENV and ZIKV infections, while critical for accurately diagnosing infection history (12, 20), may oversimplify the com- plex neutralizing antibody ...
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Characterization of Dengue Virus Complex-Specific Neutralizing Epitopes on Envelope Protein Domain III of Dengue 2 Virus
... The surface of the mature dengue virus (DENV) particle is covered with 180 envelope (E) proteins arranged as homodimers that lie relatively flat on the virion surface. Each monomer consists of three domains (ED1, ...
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Recovery of West Nile Virus Envelope Protein Domain III Chimeras with Altered Antigenicity and Mouse Virulence
... (E) protein of flaviviruses comprises three structural domains, of which domain III (EIII) represents a discrete ...E protein and to assess the effects of EIII sequence substitutions on the ...
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Biophysical Characterization and Vector-Specific Antagonist Activity of Domain III of the Tick-Borne Flavivirus Envelope Protein
... that domain III functioned as a vector-specific antagonist to interfere with flavivirus binding to host cell ...that domain III is involved in host cell receptor ...
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A NOVEL TETRAVALENT RECOMBINANT ENVELOPE DOMAIN III VACCINE AGAINST DENGUE: AN IN SILICO APPROACH
... 6. Smith SA, de Alwis AR, Kose N, Harris E, Ibarra KD, Kahle KM, Pfaff JM, Xiang X, Doranz BJ, de Silva AM, Austin SK, Sukupolvi-Petty S, Diamond MS and Crowe JE, Jr.: The potent and broadly neutralizing human dengue ...
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Type- and Subcomplex-Specific Neutralizing Antibodies against Domain III of Dengue Virus Type 2 Envelope Protein Recognize Adjacent Epitopes
... To visualize spatially the different recognition patterns of MAbs that strongly, moderately, and weakly neutralized DENV-2 infection, we docked the loss-of-binding mutations ( ⬍ 20% of wild-type binding) defined by the ...
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Comparison of Plaque- and Enzyme-Linked Immunospot-Based Assays To Measure the Neutralizing Activities of Monoclonal Antibodies Specific to Domain III of Dengue Virus Envelope Protein
... with domain III from DENV-1 to -4 as measured by ELISA and/or that recognized cells infected with different serotypes of DENV as measured by IFA from more than 100 MAbs that are available in our laboratory ...
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A Functional Epitope Determinant on Domain III of the Japanese Encephalitis Virus Envelope Protein Interacted with Neutralizing-Antibody Combining Sites
... and envelope [E]) and seven nonstructural proteins, and two nontranslating regions flank the 5⬘ and 3⬘ ends (17, 23, ...E protein has approximately 500 amino acids with six conserved disulfide bonds to ...
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Crystal Structure of the Japanese Encephalitis Virus Envelope Protein
... JEV envelope protein (E) facilitates cellular attachment and membrane fusion and is the primary target of neutralizing ...E protein possesses the three domains characteristic of flavivirus envelopes ...
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An Alphavirus E2 Membrane Proximal Domain Promotes Envelope Protein Lateral Interactions and Virus Budding
... FIG 8 HS348 and H352 interact with E1 residues. Shown are segments corresponding to the stem region of E1 (gray) and E2 (yellow) extracted from the atomic model built for the Alphavirus surface glycoprotein shell from ...
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The Chimeric Protein Domain III-Capsid of Dengue Virus Serotype 2 (DEN-2) Successfully Boosts Neutralizing Antibodies Generated in Monkeys upon Infection with DEN-2
... the domain III-capsid protein to recall the immune response gen- erated upon infection of monkeys with infective ...recombinant protein formulation, antiviral antibodies slightly increased, ...
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Interaction between duck hepatitis B virus and a 170-kilodalton cellular protein is mediated through a neutralizing epitope of the pre-S region and occurs during viral infection.
... viral envelope protein and specific cellular re- ceptor(s), is poorly ...coterminal envelope proteins from the single enve- lope gene through alternative use of in-frame AUG ...large envelope ...
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Oligomerization of Hepatitis C Virus Core Protein Is Crucial for Interaction with the Cytoplasmic Domain of E1 Envelope Protein
... membrane-associated envelope glycoproteins, E1 and E2, which assemble as a heterodimer in the endoplasmic reticulum ...the protein spans the ER membrane twice with an intervening cytoplasmic loop (amino ...
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Mechanistic Studies of a T20-Dependent Human Immunodeficiency Virus Type 1 Variant
... line, and viral spread in the culture was monitored in the presence or absence of different concentrations of antibody. Viral replication curves were measured over an 8-day period (Fig. 4). D5-IgG1, which was suggested ...
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Role of the Transmembrane Domain of Marburg Virus Surface Protein GP in Assembly of the Viral Envelope
... IEM analysis. For microscopic analysis, we used the fractions of the Nycodenz gradient containing the filamentous membranes. A drop of this suspension was deposited on Formvar-carbon-coated nickel grids for 1 h. The ...
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A Hydrophobic Domain in the Large Envelope Protein Is Essential for Fusion of Duck Hepatitis B Virus at the Late Endosome
... ies. The combined effect of a reducing agent and low pH on L conformation is reversible, and virions remain infectious upon pretreatment, in contrast to low pH treatment alone, where viri- ons become inactivated (10). ...
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Structural insights into the membrane extracted dimeric form of the ATPase TraB from the Escherichia coli pKM101 conjugation system
... T4S systems export proteins and DNA-protein complexes and fulfil a wide variety of functions, such as i- the conjugative transfer of plasmids and other mobile DNA elements to bacterial recipient cells, ii- the ...
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Identification and nucleotide sequence of the glycoprotein gB gene of equine herpesvirus 4.
... The EHV-4 gB protein contains features characteristic of all envelope glycoproteins, namely, a 5' hydrophobic signal sequence, an external hydrophilic surface domain, a hydrophobic membr[r] ...
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Sequences present in the cytoplasmic domain of CD4 are necessary and sufficient to confer sensitivity to the human immunodeficiency virus type 1 Vpu protein.
... Human immunodeficiency virus type 1 Vpu protein induces degradation of chimeric envelope glycoproteins bearing the cytoplasmic and anchor domains of CD4: role of the cytoplasmic domain i[r] ...
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Functional interactions between monomers of the retroviral envelope protein complex.
... S]cysteine (Amersham). The cells were lysed with immunoprecipitation buffer (25 mM Tris-HCl [pH 7.4], 200 mM NaCl, 6 mM 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate [CHAPS]) (Pierce, Rockford, Ill.) on ice ...
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