Enzyme cloning and purification
New Tannase-Producing Lactobacillus Sp. Nrc10: Gene Cloning, Enzyme Purification, And Characterization
... The cloning of tannase gene (Tanlp1) was carried out and its complete sequence was ...The cloning and efficient expression of tannase gene (Tanlp1) were carried ...and purification of the tannase ...
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Cloning and Expression of the algl Gene, Encoding the Azotobacter chroococcum Alginate Lyase: Purification and Characterization of the Enzyme
... the enzyme from Klebsiella aerogenes (25), ...The enzyme from ...the enzyme from ...the enzyme found in, and isolated by us from, ...the enzyme from strain 4A1M showed maximum activity ...
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4-Dihydrotrisporin-Dehydrogenase, an Enzyme of the Sex Hormone Pathway of Mucor mucedo: Purification, Cloning of the Corresponding Gene, and Developmental Expression
... mating-type-specific enzyme in the path- way from  -carotene to trisporic ...the enzyme was purified by ion exchange and affinity ...the enzyme level, activity is found exclusively in the ( ⴚ ) ...
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Cloning, Expression, Purification, and Crystallization of P aeruginosa ICMP
... It is notable that preparation of homogeneous and crystallizable proteins is the prerequisite for structural studies by crystallography. In the current study, we managed to obtain the ICMP protein that meets such a ...
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Cloning, expression and purification of Pwo polymerase from Pyrococcus woesei
... weight). Cloning was done by GATEWAY™ Cloning System and for purification of recombinant protein; His6x-Tag was added to the C-terminus of the recombinant ...polymerase enzyme by Ni-NTA ...
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Purification and cloning of the salivary peroxidase/catechol oxidase of the mosquito Anopheles albimanus
... Salivary homogenates of the adult female mosquito Anopheles albimanus have been shown previously to contain a vasodilatory activity associated with a catechol oxidase/peroxidase activity. We have now purified the ...
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Cloning, Expression and Purification of an Acetoacetyl CoA Thiolase from Sunflower Cotyledon
... Catabolism of fatty acids via the four-reaction β-oxidation cycle is a ubiquitous aspect of metabo- lism, and thiolase is the final enzyme in this cycle in both prokaryotes and eukaryotes [1]. In the final step of ...
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Purification, Cloning, and Expression of an Apyrase from the Bed Bug Cimex lectularius
... An enzyme that hydrolyzes the phosphodiester bonds of nucleoside tri- and diphosphates, but not monophos- phates, thus displaying apyrase (EC 3.6.1.5) activity, was purified from salivary glands of the bed bug, ...
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Discovery, Cloning, Expression, Purification, and Characterization of Phosphoglycolate Phosphatase from Staphylococcus aureus
... 5 aspartate or glutamate residues also serve to coordinate the M 2+ cofactor with motif 1. The amino acids within these four motifs are found in the active site (Figure 3) and it is one of the aspartates within motif 1 ...
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Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii
... Preparation and cloning of polytopic genes. The resulting sequence had a length of 405 base pairs (bp) (Fig. 1A). The pET28a vector was successful- ly prepared for insertion of the polytopic genes (Fig. 1B) and ...
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Heterologous expression, purification and enzyme activity of Neocallimastix sp. CellulaseA (celA)
... The celA gene sequence was found in GenBank, the NIH genetic sequence database (Accession number HM625672). In order to amplify the celA gene, the primers were designed corresponding to ORF of the gene. To facilitate ...
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Cloning, expression and purification of the factor H binding protein and its interaction with factor H
... Cloning of fHBP gene into pET 28a (+). The fhbp gene was selected based on the fhbp gene sequence in the GenBank accession nos. ACA52540.1. It was synthesized into pGH plasmid (Bioneer, Korea). The fhbp gene was ...
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Fast and reliable production, purification and characterization of heat-stable, bifunctional enzyme chimeras
... Fusion of genes to produce multifunctional enzymes is an interesting tool for industrial application, due to improved catalytic activity as well as lower production costs from minimized numbers of recombinant polypep- ...
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Cloning, Expression, and Purification of HIV-2 gp125
... The envelope of the human immunodeficiency virus (HIV) is the main target for neutralizing antibodies. We report the cloning, purification, and characterization of two recombinant forms of the envelope ...
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Cloning, overexpression and purification of Escherichia coli tRNAIle and tRNAVal
... tRNA Ile i utvrđivanje broja i tipa posttranskripcijskih modifikacija, biti će potrebne analize pojedinačnih nukleozida tRNA metodom spektrometrije mase... Prilikom konstrukc[r] ...
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Easy-A High-Fidelity PCR Cloning Enzyme
... PCR Cloning Enzyme N OTICE T O P URCHASER Limited Label License: The purchase of this product conveys to the purchaser only the limited, non- transferable right to use the purchased quantity of the product ...
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Purification and Properties of a Thermophilic Bacteriophage Lytic Enzyme
... phosphate concentration of at least 0.1 M is necessary for lytic enzyme stability, whereas 0.005 M is required for lytic activity. Effect ojf divalent cations and EDTA on lytic enzyme ac[r] ...
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Purification and cloning of the salivary nitrophorin from the hemipteran Cimex lectularius
... Purification, partial characterization and cloning of nitric oxide- carrying heme proteins (nitrophorins) from salivary glands of the blood-sucking insect Rhodnius prolixus. Pharmacology[r] ...
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Molecular Cloning, Expression And Purification Studies With An ORF Of Mycobacterium Tuberculosis
... for purification of expressed ...incubation. Purification analysis showed that purified protein was obtained when we used imidazole concentration at 250 ...
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Isolation and Purification of Fibrinolytic Enzyme from Microbial Source
... Pseudomonas baeticais studied for the first time as a source of fibrinolytic enzyme. KEYWORDS: Enzyme, blood clots, coagulation, baetica, RBC, WBC. I. I NTRODUCTION Blood is a bodily fluid and a connective ...
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