enzyme immunoassays
Evaluation of Four Commercially Available Epstein-Barr Virus Enzyme Immunoassays with an Immunofluorescence Assay as the Reference Method
... available enzyme immunoassays (EIAs) (Novitec, Biotest, Virotech, and DiaSorin) were evaluated, with an indirect immunofluorescence assay as the reference method, for Epstein-Barr virus (EBV) VCA (viral ...
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Comparison of two different enzyme immunoassays for detection of immunoglobulin M antibodies against tick borne encephalitis virus in serum and cerebrospinal fluid
... Two enzyme immunoassays for the detection in immunoglobulin M IgM antibodies against tick-borne encephalitis virus were compared, employing a solid phase coated either with antigen or wi[r] ...
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Earlier Detection of Human Immunodeficiency Virus Type 1 p24 Antigen and Immunoglobulin G and M Antibodies to p17 Antigen in Seroconversion Serum Panels by Immune Complex Transfer Enzyme Immunoassays
... transfer enzyme immunoassays for HIV-1 p24 antigen and antibody immunoglobulin G (IgG) to HIV-1 p17 antigen were improved approximately 25- and 90-fold, respectively, over those of the previous immuno- ...
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Rapid and Specific Detection of Toxigenic Staphylococcus aureus: Use of Two Multiplex PCR Enzyme Immunoassays for Amplification and Hybridization of Staphylococcal Enterotoxin Genes, Exfoliative Toxin Genes, and Toxic Shock Syndrome Toxin 1 Gene
... PCR enzyme immunoassays (PCR-EIAs) were developed for Staphylococcus aureus exotoxin gene screening as an alternative to the conventional biological assays, which depend on detectable amounts of toxins ...
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Antigen requirements, sensitivity, and specificity of enzyme immunoassays for measles and rubella viral antibodies
... Enzyme immunoassay EIA systems for measles virus and rubella virus were studied from the standpoints of requirements for suitable viral antigens and control antigens, and the sensitivity[r] ...
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Enzyme immunoassays in which biotinillated beta lactamase is used for the detection of microbial antigens
... Similarly, an assay in which solid-phase goat antibody to rotavirus, biotinillated chicken antibody, avidin, and biotin-labeled beta-lactamase were used could specifically detect antigen[r] ...
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Enzyme immunoassays for invasive Candida infections: reactivity of somatic antigens of Candida albicans
... Whereas all tests were able to discriminate between patients with invasive candidosis n = 27 and normal healthy volunteers n = 167, as proved by graphic marker analysis, the selective an[r] ...
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Enzyme Immunoassays for Serological Diagnosis of Bovine Brucellosis: A Trial in Latin America
... The indirect enzyme-linked immunosorbent assay (IELISA) for detection of antibody to Brucella abortus was introduced in 1976 (1). The reasons for using IELISAs were, firstly, to re- place conventional serological ...
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Are the Enzyme Immunoassays for Antibodies to Pneumococcal Capsular Polysaccharides Serotype Specific?
... The specificity of antibody binding to pneumococcal capsular polysaccharides (Pnc PSs) measured by enzyme immunoassay (EIA) was studied by inhibition of antibody binding by homologous and heterologous PSs. We ...
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Enzyme Immunoassays for Measurement of Cytomegalovirus Immunoglobulin M Antibody
... Thus, although no false-positive reactions were noted with the S-IgM system, 18% of the sera tested by the S-Ag system yielded positive results in spite of the fact that they were obtain[r] ...
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Immune complex transfer enzyme immunoassay that is more sensitive and specific than western blotting for detection of antibody immunoglobulin G to human immunodeficiency virus type 1 in serum with recombinant pol and gag proteins as antigens.
... transfer enzyme immunoassays with rRT and rp17 as antigens were 300 to 3,000 times as sensitive as Western blottings for the corresponding antigens, as described above ...transfer enzyme immunoassay ...
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Comparative Evaluation of Three Serological Methods for Detection of Human Herpesvirus 8 Specific Antibodies in Canadian Allogeneic Stem Cell Transplant Recipients
... munosorbent assay (ELISA) that uses sucrose-purified whole virus derived from the KS-1 cell line is now commercially available from Advanced Biotechnologies Inc. (ABI). It has been reported that this test is specific and ...
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Chimeric Antigens of Toxoplasma gondii: Toward Standardization of Toxoplasmosis Serodiagnosis Using Recombinant Products
... parallel enzyme immunoassays (recombinant enzyme-linked immunosorbent assays [Rec-ELISAs]), and the results obtained by standard commercial assays with the whole-cell Toxoplasma antigen and assays ...
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Use of rubella virus E1 fusion proteins for detection of rubella virus antibodies
... indirect enzyme immunoassays to detect rubella virus-specific immunoglobulin G, p1503 correctly identified the rubella virus antibody status of 43 ...
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Development of Recombinant Nucleoprotein-Based Diagnostic Systems for Lassa Fever
... as enzyme immunoassays for the detection of LASV antibodies and LASV antigens, were developed using the recombinant nucleoprotein (rNP) of LASV ...G enzyme- linked immunosorbent assay (ELISA) using ...
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Molecular basis for serological cross-reactivity between enteroviruses.
... Serum samples obtained from 58 patients with culture-confirmed enteroviral infections were analyzed in enzyme immunoassays against two sets of overlapping synthetic peptides covering res[r] ...
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Comparison of rapid detection methods for influenza A virus and their value in health care management of institutionalized geriatric patients
... Respiratory specimens from 160 geriatric patients with suspected influenza illness were used to evaluate the abilities of two enzyme immunoassays EIAs; Directigen FLU-A [Becton Dickinson[r] ...
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Comparison of nine commercial immunoassays for the detection of rotavirus in fecal specimens
... Kits evaluated included two monoclonal antibody-based enzyme immunoassays EIAs Rotaclone and Pathfinder Rotavirus, three polyclonal antibody-based EIAs Rotavirus Immunoassay, Rotazyme II[r] ...
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Coexistence of Different Genotypes in the Same Bat and Serological Characterization of Rousettus Bat Coronavirus HKU9 Belonging to a Novel Betacoronavirus Subgroup
... and enzyme immunoassays using recombinant Ro-BatCoV HKU9 nucleocapsid proteins and recombinant nucleocapsid proteins of Betacoronavirus sub- group A, B, and C coronaviruses to determine possible ...
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Detection of Staphylococcus aureus Enterotoxins A to D by Real Time Fluorescence PCR Assay
... Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exf[r] ...
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