Enzyme linked immunoassay
Evaluation of enzyme linked immunoassay for serological diagnosis of cysticercosis
... The diagnosis of cysticercosis is based on clinical, epidemi- ologic, and serologic findings. MRI and/or computed axial tomographic scans are the most sensitive and specific diagnos- tic tools (9). However, because of ...
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Pitfalls and fallacies of cat scratch disease serology: evaluation of Bartonella henselae based indirect fluorescence assay and enzyme linked immunoassay
... During the last few years it has become clear that the ma- jority of cat scratch disease (CSD) cases are caused by Bar- tonella henselae (2, 6, 10, 13, 19). Culture of B. henselae from the lymph nodes of CSD patients has ...
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Multicenter Evaluation of a New, Automated Enzyme Linked Immunoassay for Detection of Human Immunodeficiency Virus Specific Antibodies and Antigen
... microparticle-based enzyme-linked immunoassay (AxSYM HIV Ag/Ab Combo; Abbott Laboratories), designed to simultaneously detect (i) antibodies against human immunodefi- ciency virus type 1 (HIV-1) ...
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Factors involved in enzyme linked immunoassay of viruses and evaluation of the method for identification of enteroviruses
... For specific identification of a given enterovirus type by the enzyme-linked immunosorbent assay method used, approximately 105 plaque-forming units of virus per assay tube were required[r] ...
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Enzyme linked immunoassay for human immunodeficiency virus type 1 envelope glycoprotein 120
... Mixing standard amounts of reference gpl20 with increasing concentrations of human sera reduced assay sensitivity, although the linear relationship between gpl20 concentration and optica[r] ...
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Novel Enzyme Linked Immunoassay To Determine Nanogram Levels of Pneumocandins in Human Plasma
... competitive enzyme-linked immunosorbent assay (ELISA) were compromised by making use of the property of nonspe- cific binding of the semisynthetic pneumocandins to proteins and microtiter ...
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Enzyme linked immunoassay for detection of PCR amplified DNA of legionellae in bronchoalveolar fluid
... lated primers, of which most are not incorporated. The use of high-capacity streptavidin-coated microtiter plates met this de- mand and abrogated the need for first separating free from incorporated biotinylated primers. ...
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Diagnostic Accuracy of the InBios Scrub Typhus Detect Enzyme-Linked Immunoassay for the Detection of IgM Antibodies in Northern Thailand
... IgM enzyme-linked immunosorbent assay (ELISA) and determined the optimal diagnostic optical density (OD) cutoffs for screening and diagnostic applications based on prospectively collected, characterized ...
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Enzyme linked immunoassay for dengue virus IgM and IgG antibodies in serum and filter paper blood
... Table 1: Dengue antibody detection by ELISA in serum and reconstituted filter paper blood of Vietnamese febrile patients, repeated tests and storage time of specimens... Dengue tests in [r] ...
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Specific enzyme linked immunoassay for rotavirus serotypes 1 and 3
... ELISA serotype assay of coded specimens of human and animal rotavirus strains using serotype-specific monoclonal antibodies Virus species of origin.. ELISA serotype assignment.[r] ...
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Enzyme linked immunoassay for detection of Cryptosporidium antigens in fecal specimens
... Of 62 frozen stools from patients with cryptosporidiosis, as detected by at least two microscopic diagnostic techniques, 51 were positive by ELISA; all ELISA-negative specimens came from[r] ...
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Standardized quantitative enzyme linked immunoassay for antibodies to Toxoplasma gondii
... In addition, to establish a uniform basis of reference in the reporting ofresults, we measured the toxoplasma antibody levels in international units per milliliter, providing values dire[r] ...
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Immune response to a major epitope of p24 during infection with human immunodeficiency virus type 1 and implications for diagnosis and prognosis
... A sequential inhibition enzyme-linked immunoassay SIEIA using a peroxidase-conjugated monoclonal antibody reacting to the sequence AAEWDRVHP of p24HIV-1 amino acids 209 to 217 of p55 was[r] ...
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Soluble HLA measurement in saliva and cerebrospinal fluid in Caucasian patients with multiple sclerosis: a preliminary study
... Methods: We applied a solid phase, enzyme-linked immunoassay to measure soluble HLA class I (sHLA-I) and class II (sHLA-II) molecules in the saliva and cerebrospinal fluid (CSF) in 13 u[r] ...
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Identification of new rice cultivars and resistance loci against rice black-streaked dwarf virus disease through genome-wide association study
... Artificial inoculation was performed according to the method described by Zhou et al. (2015). Non-virulent SBPH were fed on plants infected with RBSDV for 3 days to make them acquire the RBSDV. The SBPH were then ...
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Evaluation of MUREX SUDS Toxo test
... Comparison of an enzyme-linked immunoassay and a quantitative indirect fluorescent-antibody test with the conventional indirect fluorescent-antibody test for detecting antibodies to Toxo[r] ...
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Rapid, Field-Adapted Indirect Enzyme-Linked Immunosorbent Assay for Detection of Antibodies in Bovine Whole Blood and Serum to Brucella abortus
... indirect enzyme-linked immunoassay (FldELISA) for the detection of anti- bodies to Brucella abortus in whole blood and serum has been ...indirect enzyme-linked immunoassay, ...
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Oxytocin is implicated in social memory deficits induced by early sensory deprivation in mice
... Enzyme-linked immunoassay analyses were performed to quantify levels of OXT and VP in the brain. The hypothalamus or the whole brain was quickly removed and frozen in liquid nitrogen. The brain ...
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Characterization of the protease and other products of amino-terminus-proximal cleavage of the herpes simplex virus 1 UL26 protein.
... The separated polypeptides from BHKTK- cells were transferred electrically to nitrocellulose membranes and reacted in an enzyme-linked immunoassay with either monoclonal antibody H725 ag[r] ...
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Detection of Histoplasma Antigen by a Quantitative Enzyme Immunoassay
... Antigen detection is a useful method for the diagnosis of histoplasmosis (8). Until now, antigen results have been ex- pressed semiquantitatively as antigen units (calculated as en- zyme immunoassay [EIA] units ...
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