Ergosterol biosynthesis
Unraveling the importance of molecules of natural origin in antifungal drug development through targeting ergosterol biosynthesis pathway
... of ergosterol are called EBIs (ergosterol biosynthesis ...the biosynthesis of ...of ergosterol synthesis in ...the ergosterol biosynthesis were done in three species of ...
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Evidence that Ergosterol Biosynthesis Modulates Activity of the Pdr1 Transcription Factor in Candida glabrata
... of ergosterol biosynthesis (as occurs in the presence of azole drugs) might trigger activation of Pdr1 ...inhibiting ergosterol biosynthesis, we demonstrated that Pdr1 activity and target gene ...
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Prediction of novel drug targets in Ergosterol biosynthesis pathway: A proposed mechanism of anticandidal activity of green tea phytocompounds
... Docking analysis was carried out for the ergosterol biosynthetic proteins with the selected phytocompounds and commercial drugs using iGEMDOCK software. The iGEMDOCK provides a graphical environment for ...
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Hydroxyurea Induces Cytokinesis Arrest in Cells Expressing a Mutated Sterol-14α-Demethylase in the Ergosterol Biosynthesis Pathway
... Recent studies have shown that HU may kill the cells by alternative mechanisms such as by generating oxidative stress (Davies et al. 2009). Here, we report the identification of a novel mutation in the erg11 gene in ...
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Protein trafficking, ergosterol biosynthesis and membrane physics impact recombinant protein secretion in Pichia pastoris
... the ergosterol and sphingolipid synthesis path- ways, which are aerobic processes requiring molecular oxygen and, therefore, were particularly affected under hypoxic ...the ergosterol biosynthesis ...
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The consequences of inhibition of ergosterol biosynthesis in sclerotinia sclerotiorum (lib) de bary by propiconazole
... inhibiting ergosterol biosynthesis but probably also by an ancillary mode by photo- oxidation of ergosterol wherein, Propiconazole acted as a photo- ...
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Overexpression or Deletion of Ergosterol Biosynthesis Genes Alters Doubling Time, Response to Stress Agents, and Drug Susceptibility in Saccharomyces cerevisiae
... terol biosynthesis pathway. This report helps to identify ergosterol biosynthesis en- zymes that have not previously been appreciated as drug ...
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The Aspergillus fumigatus Damage Resistance Protein Family Coordinately Regulates Ergosterol Biosynthesis and Azole Susceptibility
... Numerous azole-resistant A. fumigatus isolates have been found worldwide, most of which are due to point mutations in Erg11A or an alteration in the expression of its gene (40–43). This observation underscores the basic ...
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A Gain-of-Function Mutation in the Transcription Factor Upc2p Causes Upregulation of Ergosterol Biosynthesis Genes and Increased Fluconazole Resistance in a Clinical Candida albicans Isolate
... in ergosterol biosynthesis upon exposure to azole ...in ergosterol biosynthesis to be coordinately upregulated with ERG11 in a fluconazole-resistant clinical isolate compared with a matched ...
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The Multifunctional Fungal Ergosterol
... to ergosterol metabolism, as concluded from the lack of growth of 22 of the 25 strains in low calcium ...of ergosterol metabolism on fungal physiology and, consequently, reinforce the idea of the ...
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Enhancement of astaxanthin production in Xanthophyllomyces dendrorhous by efficient method for the complete deletion of genes
... the ergosterol biosynthesis, the rate-limiting enzymes in the mevalonate synthetic path- way [14] ...the ergosterol biosynthesis, leading to improved astaxanthin ...
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Quantitation of Ergosterol Content: Novel Method for Determination of Fluconazole Susceptibility of Candida albicans
... inhibiting ergosterol biosynthesis, we developed a novel method to differentiate fluconazole-susceptible from fluconazole-resistant isolates by quantitating er- gosterol production in cells grown in 0, 1, ...
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Sterol 14α demethylase mutation leads to amphotericin B resistance in Leishmania mexicana
... of ergosterol biosynthesis have been proposed to exist within a multi- enzyme complex, the ergosome ...of ergosterol synthesis leading to accumulation of 4,4-dimethylcholesta-8,14,24-trien-3β- ol and ...
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Antifungal Activity of Osthole on Microsporum canis through Interfering with Biosynthesis of Fungal Cell Wall
... of ergosterol synthesis. The content of ergosterol was detected by HPLC after the interaction of osthole with ...of ergosterol obtained was ...of ergosterol in osthole group and control group ...
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Role of Candida albicans Transcription Factor Upc2p in Drug Resistance and Sterol Metabolism
... of ergosterol bio- ...including ergosterol biosynthesis ...reduced ergosterol scavenging in those ...ERG11 ergosterol biosynthesis genes, while the homozygous deletion strain ...
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Regulation of the Hypoxic Response in Candida albicans
... In a parallel pathway, sterol depletion leads to activation of the regulators Upc2 and Ecm22, which control expression of a subset of hypoxic genes (19, 80). Upc2 and Ecm22 are paralogs resulting from the whole-genome ...
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Quantitation of ergosterol content and gene expression profile of ERG11 gene in fluconazole-resistant Candida albicans
... The tubes were further cooled to room temperature. Lipids were then extracted by addition of a mixture of 1 ml of sterile distilled water and 3 ml of n-heptane (Sigma-Aldrich, US) followed by vigorous vortex mixing for 3 ...
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Characterisation of sterol biosynthesis and validation of 14α-demethylase as a drug target in Acanthamoeba
... Sterol biosynthesis has been effectively targeted in numerous fungi using azole compounds that inhibit the cytochrome P450 enzyme sterol ...is ergosterol and identify novel putative precursors and ...
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Probable mechanism(s) of antifungal activity of SJA-95, a heptaene polyene antibiotic
... towards ergosterol of fungal cell membrane as compared to that of cholesterol of mammalian cell membrane; the binding being reversible as demonstrated by the spectral character of ...and ergosterol ...
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Concentrations of ergosterol and trichothecenes in the grains of three Triticum species
... the active substance, and they affect plants at the full flowering stage (Parry et al. 1995; Champeil et al. 2004). The significant risk posed by grain mycotoxins can be minimised only through the cultivation of ...
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