EST - SSR
EST-SSR: A New Class of Genetic Markers in Cotton
... thirty-three SSR-containing ESTs (EST-SSRs) were identified by analyzing 9,948 sequences be- longing to Gossypium hirsutum ...The EST-SSR sequences and the related EST sequences without ...
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Characterization and Development of EST-SSR Markers in Sweet Potato (Ipomoea batatas (L.) Lam)
... and EST-SSRs ...genomic SSR markers. In contrast, EST-SSRs can be rapidly developed from an EST database at lower ...Moreover, EST-SSRs can also lead to direct gene tagging for ...
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Hybrid identification and genetic variation of Elymus sibiricus hybrid populations using EST-SSR markers
... nine EST-SSR primers were used for hybrid identification based on DNA fingerprinting, and genetic diversity analysis of hybrid populations and their respective ...
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Characterization and development of 56 EST-SSR markers derived from the transcriptome of Odontobutis potamophila.
... A total of 40,905 EST sequences from the cDNA library of O. potamophila were constructed in our laboratory. We identified a total of 1,321 SSR loci, of which 200 were selected for microsatellite marker ...
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Genetic variation, population structure and linkage disequilibrium in Switchgrass with ISSR, SCoT and EST-SSR markers
... and EST-SSR primer pairs, resulting in an apparently separate cluster among the two ecotypes, confirming the genetic differences be- tween upland and lowland ...and EST-SSR to more ...
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Large-scale development, characterization, and cross-amplification of EST–SSR markers in Chinese chive
... database. SSR motifs were identified from the Chinese Chive ESTs using MISA ...of SSR in EST, the coding sequence (CDS) of each transcript was predicted by BLAST searching against NCBI non-redundant ...
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Assessment of EST SSR Markers for Evaluating Genetic Diversity in Watermelon Accessions from Zimbabwe
... EST-SSRs have recently been developed for water- melon by at least three research groups. Only 7 out of 31 loci produced polymorphism, and number of polymor- phic alleles at these loci was only one or two when ap- ...
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Genetic diversity and molecular analysis among cotton genotypes by EST-SSR markers.
... utilize EST- SSR markers for fibre quality traits to generate genetic diversity between tetraploid (Gossypium hirsutum) and diploid (Gossypium herbaceum and Gossypium arboreum) cotton species at molecular ...
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The Detection of Anaphalis spp. Genetic Diversity Based on Molecular Character (using ITS, ETS, and EST-SSR markers)
... the EST-SSR results was carried out by describing the pattern of the amplification ...with EST-SSR primers are called alleles, allele variations are the number and size of alleles ...
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Exploiting legume EST databases for the development of gene-derived SSR-markers in medicinal legume Mucuna pruriens L. (DC.)
... of EST-SSR markers and their polymorphism: From the SSR-containing ESTs, a total of 522 sequences were further shortlisted based on their presence in more than one species of which 98 were ...
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Construction of EST SSR Databases for Effective Cultivar Identification and Their Applicability to Complement for Lettuce (Lactuca sativa L ) Distinctness Test
... Cluster II consisted of cultivars with Crisphead, Romaine, Green leaf, and Butterhead, which split into two clus- ters with a mean similarity of 0.43. Cluster II-1 consisted of cultivars with Crisphead, Romaine, Green ...
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Confirmation of Pearl Millet Napiergrass Hybrids Using EST Derived Simple Sequence Repeat (SSR) Markers
... napiergrass-specific EST-SSRs were identi- fied for each PMN line, and four paternal-specific EST-SSRs conserved across all napiergrass accessions were selected to screen the putative PMN ...These ...
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Extrapolative microRNA precursor based SSR mining from tea EST database in respect to agronomic traits
... Molecular marker assisted technique in breeding programme for the selection or development of cul- tivars with desired trait from a large population is well established [5]. Among different markers used in crop ...
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An EST SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat
... However, EST-SSRs have received a lot of attention because they are derived from the transcribed region of genes responsible for the traits of ...and EST-SSRs can be rapidly developed from the in silico ...
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Comparative study of EST-SSR, SSR, RAPD, and ISSR and their transferability analysis in pea, chickpea and mungbean
... Around 20 genomic SSR and 10 EST-derived SSR primers were used for amplification study in pea. The amplification reactions were carried out in a 20 µL volume containing 1unit of Taq polymerase ...
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Effective Procedure for Development of EST SSR Markers Using cDNA Library
... Polymorphism analysis was conducted using the markers of EST-SSR primer sets developed from this study. Briefly, PCR was used to investigate genomic crane DNA using TaKaRaExTaq polymerase (TaKaRa, Shiga, ...
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Developmenrt of EST SSR and genomic SSR markers to assess genetic diversity in Jatropha Curcas L
... The EST-SSR markers comprised ...187 EST-SSRs resided in genes that are involved mainly in biological and metabolic ...Thirty-six EST-SSRs and 20 G-SSRs were chosen to analyze the genetic ...
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Assessment of EST SSR and RAPD markers for genetic analysis on Artemisia species
... five EST derived Simple Sequence Repeat (SSR) markers and 10 RAPD ...bands. SSR produced a total of 25 alleles with an average of 5 alleles per ...
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In silico search, characterization and validation of new EST SSR markers in the genus Prunus
... for EST-SSR sequences was performed using Primer3 with default parameters: optimum primer size = ...all SSR containing sequences using the ORF Finder available at NCBI using standard genetic ...the ...
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Characterization of variable EST SSR markers for Norway spruce (Picea abies L )
... Spruce EST sequences were extracted from the NCBI dbEST and screened for SSR sequences with the SciRoKo software program ...repeats). SSR motif containing sequences were subjected to clustering and ...
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