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Ex Vivo Expansion

Ex-Vivo Expansion of Fetal Cells Isolated from Maternal Circulation

Ex-Vivo Expansion of Fetal Cells Isolated from Maternal Circulation

... Prenatal diagnosis uses a variety of techniques to find out the health of the fetus. Without the information acquired by prenatal diagnosis, there could be an inappropriate result for the fetus or the mother or both. ...

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Impact of source tissue and ex vivo expansion on the characterization of goat mesenchymal stem cells

Impact of source tissue and ex vivo expansion on the characterization of goat mesenchymal stem cells

... Long-term ex vivo expansion of goat BM-MSCs may also have altered the proportion of MSC ...whether ex vivo expansion alters their amenability to genetic ...

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Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future

Platelet lysate as a substitute for animal serum for the ex-vivo expansion of mesenchymal stem/stromal cells: present and future

... the ex-vivo expansion of mesenchymal stem/stromal cells (MSCs) since platelet-derived growth factors can promote MSC ex-vivo ...

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Utilization of leukapheresis and CD4 positive selection in Treg isolation and the ex-vivo expansion for a clinical application in transplantation and autoimmune disorders

Utilization of leukapheresis and CD4 positive selection in Treg isolation and the ex-vivo expansion for a clinical application in transplantation and autoimmune disorders

... T regulatory cells (Tregs) have ability to regulate immune reaction and maintain self-tolerance attenuating autoimmunity, allergy, pathogen-induced immunopathology and over-exuberant immune responses [1–4]. Due to their ...

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Ex vivo expansion of Hematopoietic Stem cells for Therapeutic purposes: Lessons from development and the niche

Ex vivo expansion of Hematopoietic Stem cells for Therapeutic purposes: Lessons from development and the niche

... HSCs comprise a small heterogeneous pool of cells initially formed during embryogenesis to maintain the blood system through a regulated process termed hematopoiesis along the lifetime of an organism [1,2]. HSCs are ...

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Th17 cells are refractory to senescence and retain robust antitumor activity after long-term ex vivo expansion

Th17 cells are refractory to senescence and retain robust antitumor activity after long-term ex vivo expansion

... Th17 cells undergo polyfunctional conversion during expansion but do not change their interaction with host immune cells. We noticed that even without inflammatory signaling such as IL-12, which drives Th17 cell ...

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Isolation of Endothelial Progenitor Cells from Cord Blood and Induction of Differentiation by Ex Vivo Expansion

Isolation of Endothelial Progenitor Cells from Cord Blood and Induction of Differentiation by Ex Vivo Expansion

... optimal cytokine conditions required to induce the ex vivo expansion of EPCs. We found that the number of non-adherent cells was the greatest when the media supplemented with SCF, FL and TPO. ...

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Wharton’s jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells

Wharton’s jelly mesenchymal stem cell-based or umbilical vein endothelial cell-based serum-free coculture with cytokines supports the ex vivo expansion/maintenance of cord blood hematopoietic stem/progenitor cells

... HSPC expansion and interaction, such as G-CSF, GM-CSF, and CD117 ...can ex- press some common surface markers and transcrip- tion molecules, such as CD34, endomusin, VE- cadherin, CD31 (PECAM), Runx1, and ...

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Lycopene Prolongs the Lifespan and Enhances the Cytotoxicity of NK Cells after Ex Vivo Expansion

Lycopene Prolongs the Lifespan and Enhances the Cytotoxicity of NK Cells after Ex Vivo Expansion

... Abstract Lycopene is a nonprovitamin a carotenoid mainly found in fruits and vegetables, which has been reported to possess a variety of biological effects. The properties of lycopene on human natural killer (NK) cells ...

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Short term ex vivo expansion of circulating head and neck tumour cells

Short term ex vivo expansion of circulating head and neck tumour cells

... Isolated CTCs were cultured in the following formats: 96 well standard microplates, 96F non adherent microplates, spheroid microplates (Thermo Scientific, USA) and specialized microwell formats with a culture medium ...

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Biological Characteristics of Mesenchymal Stem Cells during Ex Vivo Expansion

Biological Characteristics of Mesenchymal Stem Cells during Ex Vivo Expansion

... Nevertheless, animal serum could be undesirable source of xenogeneic antigens and bear the risk of transmission of animal viruses, prions when MSC used in clinical settings. In addition, bovine proteins and peptides ...

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Original Article Ex-vivo expansion and function identification of CD4 CD25 CD127 dim regulatory T cells in severe aplastic anemia

Original Article Ex-vivo expansion and function identification of CD4 CD25 CD127 dim regulatory T cells in severe aplastic anemia

... etry-based analysis of FoxP3 expression in T cells became the gold standard for defining Treg cells. However, it then became evident that FoxP3 can also be expressed by effector T cells following activation [28], raising ...

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Ex-vivo expansion of nonhuman primate CD34+ cells by stem cell factor Sall4B

Ex-vivo expansion of nonhuman primate CD34+ cells by stem cell factor Sall4B

... 9 ex- panded primate CD34 + cells, day 9 GFP-control lentiviral transduced CD34 + cells, and day 9 GFP-Sall4B lentiviral transduced CD34 + cells, respectively ...in vivo. The cell population expanded by ...

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Hematopoietic development at high altitude: blood stem cells put to the test

Hematopoietic development at high altitude: blood stem cells put to the test

... HSCs ex vivo ...to ex vivo expansion of HSCs, Len Zon (Harvard Medical School, Boston, USA) presented work characterizing the role of prostaglandin E2 (PGE2) in hematopoietic ...

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Hematopoietic stem cells: ex-vivo expansion and therapeutic potential for myocardial ischemia

Hematopoietic stem cells: <em>ex-vivo</em> expansion and therapeutic potential for myocardial ischemia

... on ex-vivo expansion of HSCs isolated from human umbilical cord ...desired expansion volume, will the ex-vivo expanded HSCs be suitable for the treatment of IHD? Is HLA-matching ...

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Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells

Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells

... years, ex vivo expansion of HSC has been used as another approach for obtaining suf- ficient CBHSC from a single CBU in order to obtain ade- quate repopulating HSC from a single ...research, ...

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CCR7+ selected gene-modified T cells maintain a central memory phenotype and display enhanced persistence in peripheral blood in vivo

CCR7+ selected gene-modified T cells maintain a central memory phenotype and display enhanced persistence in peripheral blood in vivo

... vitro expansion, CCR7 selected post expansion or CD62L selected post expansion were incubated with target Mel-624 ...post expansion seemed to yield a slightly increased pro- portion of cells ...

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Effects of common anesthetic agents on [18F]flumazenil binding to the GABAA receptor

Effects of common anesthetic agents on [18F]flumazenil binding to the GABAA receptor

... the ex vivo groups, the average [ 18 F]flumazenil TACs in the two brain ROIs and for whole blood were obtained by averaging individually measured curves within the ...the ex vivo awake and dex ...

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Nanotoxicology of Dendrimers in the Mammalian Heart: ex vivo and in vivo Administration of G6 PAMAM Nanoparticles Impairs Recovery of Cardiac Function Following Ischemia-Reperfusion Injury

<p>Nanotoxicology of Dendrimers in the Mammalian Heart: ex vivo and in vivo Administration of G6 PAMAM Nanoparticles Impairs Recovery of Cardiac Function Following Ischemia-Reperfusion Injury</p>

... acute ex vivo or chronic in vivo treatment with naked G6 PAMAM dendrimer can signi fi cantly compromise recov- ery of non-diabetic hearts from I/R injury and can further negate the bene fi cial effects ...

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Efficient Expansion of Human Umbilical Cord Blood-
Derived NK Cells Ex Vivo without Requiring Feeder Layers

Efficient Expansion of Human Umbilical Cord Blood- Derived NK Cells Ex Vivo without Requiring Feeder Layers

... Furthermore, the establishment of UCB bank in the world now assists UCB being preserved for a long time. This leads to UCB can be used as a ready source for NK cell-based therapy. Nevertheless, although UCB contains ...

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