expressed sequence tags database
CoccoExpress: Database of Expressed Sequence Tags of Coccolithophorids
... We previously pointed out that most pages on CoccoExpress were dynamically built using data in back- end database. While planning and writing CoccoPediaLinker, we had to overcome the challenge of going through all ...
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Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger
... cDNA clones were selected randomly from the cDNA library and single-pass sequenced at the 5′ end on an ABI 3730 Genetic Analyzer (Applied Biosys- tems). A large-scale EST sequencing project for Sibe- rian tiger was ...
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Analysis of expressed sequence tags in developing secondary xylem and shoot of Acacia mangium
... In total, 10 752 independent colonies of the cDNA library were isolated and inoculated onto 384-well plates contain- ing culture medium. The inserts were amplifi ed by PCR, and the PCR products were used as templates for ...
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Generation, analysis and functional annotation of expressed sequence tags from the ectoparasitic mite Psoroptes ovis
... To identify signalling and metabolic pathways enriched within the P. ovis EST set, annotated sequences were mapped against the KEGG pathway database [52]. This analysis identified 78 enriched pathways within the ...
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Use of expressed sequence tags as an alternative approach for the identification of Taenia solium metacestode excretion/secretion proteins
... decoy database with 185,700 reversed sequences was cre- ated using ...final database. Database searching with X!Tandem ...dundant database (E-value < 10 −10 ) and for each rec- ognized EST, ...
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Expressed sequence tags from Atta laevigata and identification of candidate genes for the control of pest leaf cutting ants
... protein database; E-value ≤ 10 -5 ), Gene Ontology (GO) terms [34], InterProScan classification [35,36] including signal peptide [37] and transmembrane regions predictions, Kyoto Encyclopedia of Genes and Genomes ...
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In silico identification and target prediction of micrornas in Sesame (sesamum indicum L.) expressed sequence tags
... The prediction of targets for the identified miRNAs was expected to help us for clarification of the important function and regulation of these novel miRNAs in S. indicum. Most plant miRNAs are perfectly or near- ...
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Characterization of simple sequence repeats (SSRs) from Phlebotomus papatasi (Diptera: Psychodidae) expressed sequence tags (ESTs)
... Simple sequence repeats (SSRs), or microsatellites, are common in eukaryotic genomes and are short, repeated nucleotide sequence elements arrayed in tandem and flanked by non-repetitive ...of ...
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Analysis of Simple Sequence Repeats Information from Floral Expressed Sequence Tags Resources of Papaya (Carica papaya L )
... Complete papaya genome has been sequenced by Ming et al. [5], which gen- erated enormous amount of ESTs and other DNA sequences which are freely accessible at NCBI (http://www.ncbi.nlm.nih.gov) and the availability of ...
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Pilot survey of expressed sequence tags (ESTs) from the asexual blood stages of Plasmodium vivax in human patients
... both BLASTN and BLASTX was used to define a significant database match. Considering this E-value, 641 cluster events were identified by similarity, whereas 25 did not meet this criterion and were considered ...
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Chromosome Bin Map of Expressed Sequence Tags in Homoeologous Group 1 of Hexaploid Wheat and Homoeology With Rice and Arabidopsis
... pw.usda.gov/cgi-bin/westsql/map_locus_rev.cgi. An anomaly was defined as an EST having loci mapped to Data analysis: Only mapping data validated by three persons nonoverlapping bins on homoeologous chromosomes (Munk- ...
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A Comparative Analysis of Gene Expression Profile in Liver and Esophageal Cancer using Expressed Sequence Tags
... Methods: EST profiles of protein-coding genes in normal and cancerous hepatic and esophageal tissues were extracted from the UniGene database. Genes with > 1500 transcripts per million were selected as highly ...
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SpiroESTdb: a transcriptome database and online tool for sparganum expressed sequences tags
... online database of SpiroESTdb also provides com- prehensive information on the ESTs through the “Anno- tation” section, which consists of 11 categories including Statistics, Annotation Report, BLASTN, BLASTX, ...
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Expressed sequence tags related to nitrogen metabolism in maize inoculated with Azospirillum brasilense.
... NR in maize (ZmNR) F (forward) - 5'-GCCGAGTCCGACAATTACTAC-3' and R (reverse) - 5'-GCGTCGTTATCACCGAGTTTA-3', and of glutamine synthetase in maize (ZmGln1-3) F-5'-GTGGTATGGTATTGAGCAGGAG-3' and ...
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Generation and analysis of expressed sequence tags from a cDNA library of the fruiting body of Ganoderma lucidum
... showed sequence similarities to unchar- acterized genes encoding hypothetical proteins that were omitted from the ...significant sequence simila- rities to at least one sequence in the Nr and Nt ...
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Simple sequence repeats in zebra finch (Taeniopygia guttata) expressed sequence tags: a new resource for evolutionary genetic studies of passerines
... The most common motif among EST-SSRs ≥20 bp or ≥30 bp was the dinucleotide AT. Similar observations have been made in rice [36] and several species of pine [35], but this pattern is by no means consistent across species ...
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Large-scale development, characterization, and cross-amplification of EST–SSR markers in Chinese chive
... coding sequence (CDS) of each transcript was predicted by BLAST searching against NCBI non-redundant protein sequence and Swiss Prot protein databases, as well as by EST ...
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Gene discovery in Triatoma infestans
... protein sequence and protein domain databases, EC numbers assignation and GO term mapping were carried out using the Blast2GO software [49] (Additional file 2: Table ...
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Immunoglobulin E Reactivity of Recombinant Allergen Tyr p 13 from Tyrophagus putrescentiae Homologous to Fatty Acid Binding Protein
... Korea. Expressed sequence tags were analyzed to identify possible storage mite allergens, and the cDNA sequence encoding a protein homologous to fatty acid binding protein, a mite group 13 ...
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Differences in transcription between free-living and CO2-activated third-stage larvae of Haemonchus contortus
... Advances in genomic, proteomic and bioinformatic technologies [19-22] now provide opportunities for exploring the molecular basis of developmental switches in H. contortus and other nematodes. In particular, the combined ...
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