Expressing the recombinant protein
Differential humoral and cellular immunity induced by vaccination using plasmid DNA and protein recombinant expressing the NS3 protein of dengue virus type 3
... multifunctional protein playing a major role during viral ...served protein among the different dengue serotypes, which elicits a strong cellular immune response after viral infection in humans and animal ...
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Generation of a Recombinant Akabane Virus Expressing Enhanced Green Fluorescent Protein
... the recombinant virus, although this strategy does not guarantee the same expression levels of viral proteins as those of the parent ...of recombinant RVFVs expressing foreign genes, such as ...
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Attenuation of Recombinant Yellow Fever 17D Viruses Expressing Foreign Protein Epitopes at the Surface
... envelope protein and in the identification of insertion sites therein for the expression of heterologous peptide epitopes ...E protein carrying the nonapeptide CD8 ⫹ epitope SYVPSAEQI, derived from the CS ...
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Apical Budding of a Recombinant Influenza A Virus Expressing a Hemagglutinin Protein with a Basolateral Localization Signal
... M1 protein can interact with membranes (8, 16, 17, 25) and that this protein appears to provide the budding force required for virus particle formation (14, 26), the signals responsible for the transport of ...
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In Vitro and In Vivo Infection of Neural Cells by a Recombinant Measles Virus Expressing Enhanced Green Fluorescent Protein
... fluorescent protein (EGFP) (19) to infect human astrocytoma cells and observed spread from cell to cell via the interconnecting as- trocytic process ...The recombinant virus, MVeGFP, is based on the ...
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Identification of Infected B-Cell Populations by Using a Recombinant Murine Gammaherpesvirus 68 Expressing a Fluorescent Protein
... of recombinant viruses harboring the HCMV- driven YFP expression cassette with or without flanking insu- lator sequences have not shown any significant differences in the levels of YFP expression or the duration ...
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SLAM (CD150)-Independent Measles Virus Entry as Revealed by Recombinant Virus Expressing Green Fluorescent Protein
... and/or quality of MV envelope proteins because of their nature as a heterologous virus system. While IC323-EGFP produced large syncytia exhibiting green autofluorescence in SLAM-positive cells, it hardly spread from the ...
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A novel method for monitoring Mycobacterium bovis BCG trafficking with recombinant BCG expressing green fluorescent protein.
... marker protein, is attracting widespread interest because of its tremendous potential for in vivo gene expression and protein localization, cell lineage de- termination, and in situ monitoring of cell ...
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Development and Characterization of A Recombinant Orf Virus Vector Expressing the Spike Protein of Porcine Epidemic Diarrhea Virus
... the recombinant ORFV- PEDV-S protected pigs from clinical PED and reduced virus shedding following oral challenge- ...the recombinant ORFV-PEDV-S elicits secretory IgA (sIgA) responses at mucosal surfaces, ...
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Generation and characterization of a recombinant Newcastle disease virus expressing the red fluorescent protein for use in co infection studies
... the recombinant virus generated here (rLS-RFP) to determine the mechanism responsible for super infection exclusion in NDV and to as- sess the potential for recombination among different NDV ...
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Construction and Immunogenicity of Recombinant Adenovirus Vaccines Expressing the HMW1, HMW2, or Hia Adhesion Protein of Nontypeable Haemophilus influenzae
... high-titer recombinant adenoviruses expressing the HMW1, HMW2, or Hia ...purified recombinant adenoviruses for further investigation and ...purified recombinant adenoviral constructs were ...
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Evaluation of protective efficacy conferred by a recombinant Mycobacterium bovis BCG expressing a fusion protein of Ag85A-ESAT-6
... Discussion Previous research revealed that the rBCG-AE strain is able to express the Ag85A-ESAT-6 fusion protein, whereas the BCG strain could not secrete this protein. This suggests that the rBCG-AE strain ...
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Recombinant Mycobacterium smegmatis expressing Hsp65–hIL–2 fusion protein and its influence on lymphocyte function in mice
... shock protein 65 (Hsp 65 ) is a highly immunogenic protective antigen that is important in MTB ...fusion protein containing Objective: To construct a strain of recombinant Mycobacterium smegmatis ...
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Recombinant baculovirus expressing the FrC-OVA protein induces protective antitumor immunity in an EG7-OVA mouse model
... G protein (VSVG) and heterologous peptide/protein via the GP64 anchor are the most widely adopted methods for enhan- cing the in vitro and in vivo gene transduction efficiency of recombinant ...
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Viral liposomes released from insect cells infected with recombinant baculovirus expressing the matrix protein of vesicular stomatitis virus.
... 1A, vAcVSV-M recombinant baculovirus infection of SF9 cells expressed large amounts of a stainable protein that comigrated with virion M protein at -34 kDa; the amount of stainable M pro[r] ...
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Luxury at a Cost? Recombinant Mouse Hepatitis Viruses Expressing the Accessory Hemagglutinin Esterase Protein Display Reduced Fitness In Vitro
... We propose that the reduction of in vitro propagation effi- ciency associated with the expression of wild-type HE is related to the incorporation of this protein into virus particles. HE might exert this effect at ...
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Recombinant Avian Infectious Bronchitis Virus Expressing a Heterologous Spike Gene Demonstrates that the Spike Protein Is a Determinant of Cell Tropism
... Sequence analysis of the M41-CK S gene identified 72 nt differences from the Beau-R S gene sequence, of which 50 represented nonsynonymous substitutions and 22 represented synonymous substitutions, resulting in a total ...
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Observation of Measles Virus Cell-to-Cell Spread in Astrocytoma Cells by Using a Green Fluorescent Protein-Expressing Recombinant Virus
... the recombinant virus, MVeGFP, was used to infect GCCM cells in order to assess the distribution of EGFP and viral antigens with respect to the ...reporter protein to any particular area of the nucleus, or ...
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Compartmentalization of VP16 in Cells Infected with Recombinant Herpes Simplex Virus Expressing VP16-Green Fluorescent Protein Fusion Proteins
... multifunctional protein we re- port the construction and analysis of recombinant viruses ex- pressing the VP16 linked to the green fluorescent protein ...fusion protein into the ...
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A Recombinant Rhesus Cytomegalovirus Expressing Enhanced Green Fluorescent Protein Retains the Wild-Type Phenotype and Pathogenicity in Fetal Macaques
... the recombinant virus were compared to those of wild-type RhCMV to determine whether the insertion of the EGFP cassette into the RhCMV genome perturbed viral replication in ...
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