expression vectors
Development of expression vectors for Escherichia coli based on the pCR2 replicon
... the vectors were found to be 100% stable over a large number of generations in the absence of selection ...an expression plasmid ...the expression plasmid pCR2-IFNγ and using a dual plasmid heat ...
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Constitutive and regulated expression vectors to construct polyphosphate deficient bacteria
... of expression vectors with constitutive and regulated promoters that overexpress in bacteria the yeast PPX1 fused to a hexa histidine-tag (6 ...inducible expression of PPX1 removed almost all ...
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A novel pair of inducible expression vectors for use in Methylobacterium extorquens
... inducible expression vectors for use in ...these vectors lies in their use of two separate transcriptional repressors, TetR and CymR, along with a strong promoter from the rhizobial phage ...
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In vitro analysis of expression vectors for DNA vaccination of horses: the effect of a Kozak sequence
... mammalian expression vectors were com- pared for their ability to drive high levels of HSA protein expression in four different primary horse cells and COS- ...the vectors, G1 and H1 with the ...
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Development of expression vectors based on pepino mosaic virus
... TMV vectors [63], they had little impact in the case of PepMV: the hybrid vector suffered trans- gene loss at 7-10 dpi, similarly as with the duplicated SGP ...
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Recombination ready Sindbis replicon expression vectors for transgene expression
... for expression clone screening that looks for genes introducing a new phenotype into a cell line, such as producing resistance to viral infection in a previously susceptible cell ...cDNA expression ...
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Positive regulation of Shigella flexneri virulence genes by integration host factor
... constitutive expression of the virF or virB genes from expression vectors confirm that the binding of IHF to the virB promoter plays a direct transcriptional role in vivo and that its effect on ...
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Original Article CD13 overexpression promotes rectal cancer cell proliferation by increasing VEGF expression
... the expression level of cluster of differentiation 13 (CD13) in rectal cancer tissues and explore the effect of CD13 overexpression on the biological behavior of rectal cancer ...Methods: Expression of CD13 ...
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A versatile vector system for generating recombinant EGFP tagged proteins in yeast
... Construction of the recombinant plasmids pBS(GFP) was performed as followed. Five micrograms of the ret- roviral plasmid PINCO [25], carrying the EGFP cDNA, were digested with Hind III and Not I overnight. The EGFP cDNA ...
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Characterisation of PP2Cβ in regulating tumour suppressor pathways in cancer cell lines : A thesis presented to Massey University in partial fulfilment of the requirement for the degree of Masters in Genetics
... gene expression based on a modular relational database that integrated microarray results with a genome-wide search of p53 family member response ...gene expression. Mammalian expression ...
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Upregulation of SATB1 is associated with the development and progression of glioma
... the expression of SATB1 is correlated with MGMT promoter methylation which is a key prognostic factor and can predict treatment response in glioma ...SATB1 expression correlated with poor prognosis in ...
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Coronaviruses as Vectors: Stability of Foreign Gene Expression
... as expression vectors has also been studied to some ...as vectors are the absence of nonessential genes, the overlap of all adjacent ORFs, and the consequent embedding of each TRS within the ...
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Split-Intron Retroviral Vectors: Enhanced Expression with Improved Safety
... retrovirus-based expression vectors leads to a fraction of the resulting transcripts being ...these vectors to produce enhanced expression from near fully spliced (and thus packaging signal ...
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BglBrick vectors and datasheets: A synthetic biology platform for gene expression
... these vectors for further applica- tion, various additional biological parts can be designed with the BglBrick ...BglBrick vectors or to BglBrick plasmids already con- taining pathway genes to allow ...
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Effect of the E4 region on the persistence of transgene expression from adenovirus vectors.
... adenovirus vectors for gene therapy is limited by the transience of expression that has been observed in various in vivo ...transfer vectors based on Ad2E4ORF6 would have a reduced potential for ...
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Human immunodeficiency virus vectors for inducible expression of foreign genes.
... To determine whether H5 HA expression could be detected following infection with GB112, virus was produced via COS cell transfection as described above by using GB111 or GB112 as the vec[r] ...
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Design of retrovirus vectors for transfer and expression of the human beta-globin gene.
... Retrovirus vectors currently offer the best vehicle for gene transfer into marrow cells, and vectors carrying the P-globin gene have been used to transfer the ,-globin gene into murine e[r] ...
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Construction of Retroviral Vectors with Improved Safety, Gene Expression, and Versatility
... gene expression from several different types of retroviral vectors currently used in gene therapy clinical trials ...gene expression after transduction of a variety of target cells, consistent with ...
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Role of N Glycosylation of Hepatitis B Virus Envelope Proteins in Morphogenesis and Infectivity of Hepatitis Delta Virus
... In this study, we used a site-directed mutagenesis approach to eliminate N-linked glycosylation codons on the three HBV envelope proteins and we analyzed the effects of the removal of N glycans on (i) subviral HBV ...
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The Potyvirus Silencing Suppressor Protein VPg Mediates Degradation of SGS3 via Ubiquitination and Autophagy Pathways
... the expression of viral suppressors of RNA silenc- ing ...the expression of VPg alone, independent of viral infection, is sufficient to induce the degrada- tion of SGS3 and its intimate partner RNA-dependent ...
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