Extractable Nuclear Anti-Antigens
The MRZ reaction helps to distinguish rheumatologic disorders with central nervous involvement from multiple sclerosis
... measurements. Anti- nuclear antibody (ANA) staining pattern was assessed using indirect immunofluorescence (IIF) on 2100-Ro HEp-2000® cells (Fluorescent ANA-RoTest System, Immuno Concepts ...against ...
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Detection of Anti Extractable Nuclear Antigens in Patients with Systemic Rheumatic Disease via Fluorescence Enzyme Immunoassay and Its Clinical Utility
... of anti-RNP, the diagnoses and semi-quantitative antibody titers, described as positive or weak positive, varied among the cases: SLE (weak positive, n=2; positive, n=2), SjS (weak positive, n=2; positive, n=2), ...
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Elevated expression of TIGIT on CD3+CD4+ T cells correlates with disease activity in systemic lupus erythematosus
... DNA; Anti-SSA: anti-SSA antigen; Anti-SSB: anti-SSB antigen; APC: antigen-presenting cells; C3: complement 3; C4: complement 4; CD40L: CD40 ligand; CTLA-4: cytotoxic T-lymphocyte-associated ...
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Ultraviolet light induces binding of antibodies to selected nuclear antigens on cultured human keratinocytes
... of extractable nuclear antigens (SSA/Ro, RNP, and Sm) following irradiation was seen on injured keratinocytes whose cell membranes were intact, but not on dead ...monospecific anti-SSA/Ro sera ...
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Practical Evaluation of Methods for Detection and Specificity of Autoantibodies to Extractable Nuclear Antigens
... enzyme-linked immunoassay (ELISA) systems have been de- veloped to detect and determine the specificity of anti-ENA antibodies. ELISA systems permit more rapid processing of more specimens with a faster turnaround ...
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Scl 86, a marker antigen for diffuse scleroderma
... of anti-Scl-86 antibodies seemed to correlate with the diagnosis of diffuse scleroderma since they were found in 13 out of 22 diffuse scleroderma patients and in only one out of 11 patients with limited ...of ...
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Antibodies against specific extractable nuclear antigens (ENAs) as diagnostic and prognostic tools and inducers of a profibrotic phenotype in cultured human skin fibroblasts: are they functional?
... with anti-Topo-I antibodies (usually DcSSc patients) are linked with a higher prob- ability of interstitial lung disease (ILD), renal vascular damage, renal crisis, and heart fibrosis ...of ...
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Risk factors for ANA positivity in healthy persons
... The extractable nuclear antibody (ENA) panel for eight additional speci- ficities was a Luminex-based multiplex assay, and posi- tive results were as defined by the manufacturer ...auto- antigens and ...
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Decreased frequency and activated phenotype of blood CD27 IgD IgM B lymphocytes is a permanent abnormality in systemic lupus erythematosus patients
... Ab: anti- body; ANA: anti-nuclear antibodies; BCR: B cell receptor; DN: double negative; ENA: extractable nuclear antigens; FC: flow cytometry; FITC: fluorescein isothio- ...
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Detection of antibodies to extractable nuclear antigens by ELISA for the diagnosis of connective tissue disorders in ANA positive patients
... to antigens that are applied as distinct lines on a ...Specific nuclear antigens are applied to nitrocellulose strips at equal ...the antigens are traced by alkaline phosphatase conjugated ...
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Autoantibodies to Extractable Nuclear Antigens: Making Detection and Interpretation More Meaningful
... It is clear that no ideal test exists that is both highly sensitive and highly specific for anti-ENA antibodies. The European Consensus Workshops recommended that ENA testing be per- formed by two or more methods ...
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Predictive biological markers of systemic lupus erythematosus flares: a systematic literature review
... ANA: Anti-nuclear antibodies; Anti- dsDNA ab: Anti-double-stranded DNA antibodies; Anti-ENA: Antibodies against extractable nuclear antigens; Anti-RNP: ...
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Immune complexes containing scleroderma specific autoantibodies induce a profibrotic and proinflammatory phenotype in skin fibroblasts
... metalloproteinase (MMP)-2, tumor growth factor (TGF)- β 1 and Pro-CollagenI α 1 secretion, collagen (col)I α 1, mmp-1, toll-like receptor (tlr)2, tlr3, tlr4, tlr7, tlr8, tlr9, interferon (ifn)- α , ifn- β and ...
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Detection of Antibodies to Candida albicans Germ Tubes during Experimental Infections by Different CandidaSpecies
... the anti- genic composition of the fungus occur which may be useful for serodiagnosis of invasive ...with antigens expressed specifically or predominantly on the germ tube cell wall surface (6, 10, 25, 29, ...
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Cellular Compartmentalization of Herpesvirus Antigens During Viral Replication
... Three small nuclear granules, large nuclear granules, and an amorphous mass filling the nucleus contain antigens which react with a rabbit serum prepared against boiled infected cell deb[r] ...
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Monteith_unc_0153D_15868.pdf
... and nuclear antigens to leak into the cytosol and activate innate ...of nuclear antigens within the phagolysosome activates intracellular TLRs, we quantified IRAK1 levels in MFs 24 hours after ...
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Combined Fluorescent-Antibody and Electron Microscopy Study of Marek's Disease Virus-Infected Cell Culture
... Diffuse and granular cytoplasmic antigens are seen in round cells and fusiform cells inset.. The nuclear staining is not as bright as the cytoplasmic staining.[r] ...
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Immediate determination of ACPA and rheumatoid factor a novel point of care test for detection of anti MCV antibodies and rheumatoid factor using a lateral flow immunoassay
... for anti-MCV antibodies and ...test anti-MCV and RF-IgM, the only laboratory classification criterion for RA to ...of anti-MCV may be due to the fact that almost all patients have been under ...
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Epstein Barr Virus Antibodies in Kawasaki Disease
... concerning anti-VCA ...was anti-VCA IgM- positive in the acute stage (within 1-2 ...was anti-VCA IgM-positive at one week seroconverted to anti-VCA IgG- and anti- EADR-positive after ...
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The inclusion of N terminal pro brain natriuretic peptide in a sensitive screening strategy for systemic sclerosis related pulmonary arterial hypertension: a cohort study
... Abbreviations 6MWD: Six-minute walk distance; ANA: Anti-nuclear antibody; anti-cent: Anti-centromere antibody; anti-Scl70: Anti- topoisomerase-1 antibody; ASCS: Australian Scleroderma Co[r] ...
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