Fab fragment
Crystal structure of signal regulatory protein gamma (SIRPγ) in complex with an antibody Fab fragment
... the Fab fragment of a SIRP monoclonal antibody (OX117) that had previously been generated ...Both Fab frag- ments of OX117 and the extracellular region of SIRPγ were produced as recombinant proteins ...
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Generation of Fab Fragment-Like Molecular Recognition Proteins against Staphylococcal Enterotoxin B by Phage Display Technology
... A Fab-displayed phage library was constructed by using cDNA from the spleens of immunized ...SEB-reactive Fab-displaying phages were then screened. The Fab fragment was converted into scFv as ...
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Minute Virus of Mice, a Parvovirus, in Complex with the Fab Fragment of a Neutralizing Monoclonal Antibody
... neutralizing Fab fragment of the mouse monoclonal antibody (MAb) B7 was determined by cryo-electron microscopy to 7-Å ...The Fab molecule recognizes a conformational epitope at the vertex of a ...
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In vivo characterization of the novel CD44v6-targeting Fab fragment AbD15179 for molecular imaging of squamous cell carcinoma: a dual-isotope study
... a Fab fragment is an antibody structure of about 50 kDa that still binds to antigens but is monovalent with no Fc portion, whereas Fab 2 fragment antibodies have two ...
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Production and Characterization of a Human Recombinant Monoclonal Fab Fragment Specific for Influenza A Viruses
... Conclusions. In this work a human recombinant MAb has been described, which demonstrated a significant specificity only to FLU-A viral strains. It was obtained using combinato- rial phage display technology, which can be ...
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Double mutation of cell wall proteins CspB and PBP1a increases secretion of the antibody Fab fragment from Corynebacterium glutamicum
... antibody Fab fragment, a heterodimeric mo- lecule containing an intermolecular disulfide bond, using the CORYNEX® ...combinant Fab(H+L) was secreted into the culture medium by the industrial strain ...
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Current applications of antibody microarrays
... fragments; Fab: fragment antigen‑binding; CD: cluster of differentiation; ELISA: enzyme‑linked immunosorbent assays; DNA: deoxyribonucleic acid; MS: mass spectrometry; CB: calbindin‑D28 k; AD: Alzheimer’s ...
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Trastuzumab- and Fab′ fragment-modified curcumin PEG-PLGA nanoparticles: preparation and evaluation in vitro and in vivo
... that Fab ′ fragment, which was prepared by digesting the intact antibody, was modified to a nano-vector for drug delivery to overcome the defects of TMAB ...that Fab ′ -NPs exhibited enhanced ...
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A Similar Pattern of Interaction for Different Antibodies with a Major Antigenic Site of Foot-and-Mouth Disease Virus: Implications for Intratypic Antigenic Variation
... the Fab fragment of MAb ...The Fab fragment was obtained by digestion with soluble papain in phosphate-buffered saline ...The Fab moiety was purified by protein A- Sepharose ...
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Antibody-mediated targeting of iron oxide nanoparticles to the folate receptor alpha increases tumor cell association in vitro and in vivo
... a fab fragment of Trastuzumab, and it was determined then that the free heavy and light chains resulted from transient reduction of the intrachain disulfide bond, with no significant loss of binding ...
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Immunofluorescence Triple Lindy
... 2. Unconjugated Rabbit anti-mouse Fab fragment FOLLOWED BY anti-rabbit Alexa conjugated secondary.. Method Example: Beta Catenin.. Beta Catenin DAPI. Mouse antibody on mouse tissue: M.[r] ...
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Improvement of Fab expression by screening combinatorial synonymous signal sequence libraries
... of Fab fragment by harmonizing selected segments in the Fab fragment gene ...a Fab fragment on protein expression in the peri- ...with Fab fragments expressed from a ...
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Crumbs 3b promotes tight junctions in an ezrin dependent manner in mammalian cells
... Description Chain L, Structure Of Dengue Virus Diii In Complex With Fab 2h12 Chain L, Opg2 Fab Fragment keratin 1 [Homo sapiens] actin, cytoplasmic 1 [Homo sapiens] Ig kappa chain V regi[r] ...
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Fiedler, Luise (2019): Diagnostic and therapeutic applications of CA XII targeting 6A10 Fab: radiochemical and biological studies. Dissertation, LMU München: Medizinische Fakultät
... 6A10 Fab Fragment bindet spezifisch an CA XII, weshalb es als Trägermolekül eines radioaktiven Nuklides prädestiniert ...das Fab Fragment mit Kupfer- 64 markiert und damit erste in-vivo Daten ...
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High-Resolution X-Ray Structure and Functional Analysis of the Murine Norovirus 1 Capsid Protein Protruding Domain
... a Fab fragment and the crystal struc- tures of the P domain into the cryoelectron microscopy three- dimensional (3D) reconstruction of the Fab/MNV-1 complex indicated that the “closed” conformation ...
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Human Antibody Responses to Mature and Immature Forms of Viral Envelope in Respiratory Syncytial Virus Infection: Significance for Subunit Vaccines
... of Fab fragment RSV19 to inhibit the binding of RSV antibodies in human serum to RSV-infected cells was ...one Fab fragment (CM68) was biotinylated with a biotin labeling kit (Boehringer ...
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The cosmology of the Fab Four
... The layout is as follows: in section II we briefly recap the key Hamiltonian and scalar field equations of motion for the Fab-Four system arising from the original action of Horndeski [3] that is minimally coupled ...
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Structural Basis of Human Parechovirus Neutralization by Human Monoclonal Antibodies
... AM28 Fab arm appears to inter- act with epitopes from neighboring ...the Fab alone can neutralize the virus but not cause the aggregation seen with the MAb ...
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Effect of culture medium, host strain and oxygen transfer on recombinant Fab antibody fragment yield and leakage to medium in shaken E. coli cultures
... on Fab expres- sion has also been studied in ...highest Fab yields were obtained by induction with either a very low IPTG concentration or 2 g l -1 lac- tose using glycerol as the main carbon source ...
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A CD19/Fc fusion protein for detection of anti CD19 chimeric antigen receptors
... product was digested with EcoR1. The digested products were ligated into the Fc fragment-containing expression plasmid (pCMV-Fc), previously digested with NheI and EcoR1 to create either pCMV-CD19sIg1-3 or pCMV- ...
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