FACS, fluorescence-activated cell sorting
Maintaining RNA Integrity for Transcriptomic Profiling of Ex Vivo Cultured Limbal Epithelial Stem Cells after Fluorescence-Activated Cell Sorting (FACS)
... the sorting of heterogeneous cell populations, we rely on both extracellular and intracellular markers, and therefore need to fix and permeabilize the cells prior to staining and ...
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CD15 Expression Does Not Identify a Phenotypically or Genetically Distinct Glioblastoma Population.
... CD152 cell popu- lations exposed to the same environmental conditions in vivo to Figure ...by fluorescence-activated cell sorting (FACs) established a phenotypic equilibrium that ...
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A systematic review of CD14 and toll-like receptors in relation to asthma in Caucasian children
... Assay, FACS: Fluorescence-Activated Cell Sorting, FBAT: Family-Based Association Test, GEE: Generalized estimating equations, HWE: Hardy Weinberg Equilibrium, HPLC: High-Performance ...
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Spontaneous opticospinal encephalomyelitis in a double transgenic mouse model of autoimmune T cell/B cell cooperation
... The majority of these opticospinal EAE OSE mice had a paralytic disease caused by inflammatory demyelinating Nonstandard abbreviations used: FACS, fluorescence-activated cell sorting; Ig[r] ...
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Capturing Alzheimer's disease genomes with induced pluripotent stem cells: prospects and challenges
... identified cell surface molecular signatures that allow the purification of neural precursor cells (NPCs) and neurons from differentiated hESCs by fluorescenceactivated cell sorting ...stem ...
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Combination of BCL11A siRNA with vincristine increases the apoptosis of SUDHL6 cells
... a fluorescence microscope (Leica, ...to fluorescence-activated cell sorting (FACS) analysis (BD FACS Calibur, Franklin Lakes, New Jersey, ...
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A novel anti-EMMPRIN function-blocking antibody reduces T cell proliferation and neurotoxicity: relevance to multiple sclerosis
... Using fluorescence-activated cell sorting (FACS) analysis (A), mouse astrocytes identified by glial fibrillary acidic protein (GFAP) were examined for extracellular matrix ...
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Biological Signature Characteristics of Primary Isolates from Human Immunodeficiency Virus Type 1 Group O in Ex Vivo Human Tonsil Histocultures
... proteins. Fluorescence-activated cell sorting (FACS) analysis was used to measure the ratio of CD4 T cells to CD8 T cells in infected and uninfected cultures, ...(A) FACS dot ...
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The proliferation and differentiation of osteoblasts in co-culture with human umbilical vein endothelial cells: An improved analysis using fluorescence-activated cell sorting
... inhibits cell proliferation and promotes cell ...higher cell density. After 120 h, the total cell number of MG- 63 cells in the single culture was about twice as high as that in the ...the ...
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Functional characterisation of human pulmonary monocyte-like cells in lipopolysaccharide-mediated acute lung inflammation
... 2 Fluorescence activated cell sorting of cells in BAL fluid following LPS ...using FACS with >95% purity, and overlapped on SSC-A versus FSC-A dot plots are seen in ...
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Notochordal cell conditioned medium stimulates mesenchymal stem cell differentiation toward a young nucleus pulposus phenotype
... candidate cell type for tissue regeneration, to a phenotype with characteristics of the developing ...NC cell con- ditioned medium will promote differentiation of MSCs toward a generally chondrogenic ...
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Regulatory roles of interferon-inducible protein 204 on differentiation and vasculogenic activity of endothelial progenitor cells
... of cell growth and ...Using fluorescence-activated cell sorting, immunocytochemistry, and quantitative PCR, endothelial markers including CD31, VE-cadherin, and vWF were detected in the ...
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Girls homozygous for an IL 2–inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV associated lymphoproliferation
... In mice, NKT cells have been shown to provide a protective innate-type immune response to several microbes (27) includ- ing viruses, e.g., HSV1 and HSV2 (28, 29) and hepatitis B virus (30). Strong evidence for a ...
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Thesis
... Thickness of the PDMS arrays was varied for the 200 µm x 200 µm x100 µm templates by using the COC spacers as described above. Array thicknesses that were studied were 100 µm, 200 µm, 300 µm, 400 µm, and 1,000 µm. Three ...
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Invasive oral cancer stem cells display resistance to ionising radiation
... stem cell-like enriched populations of nasopharyngeal carcinoma cells, c-MYC positively regulates the expression of CHK1 and CHK2 to confer radioresistance [30], supporting the idea that an enhanced DDR may confer ...
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ATR maintains chromosomal integrity during postnatal cerebellar neurogenesis and is required for medulloblastoma formation
... We found that ATR-deficient CGNPs continued to proliferate despite accumulating DNA damage, which induced population- wide, p53-dependent apoptosis. Blocking apoptosis in ATR- deficient CGNPs through co-deletion of the ...
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Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
... A tetracycline-inducible vector (TetO-HHFG) was con- structed based on the TetO-FUW-eGFP plasmid where gene expression is controlled from seven copies of the tetracycline operator (7xTetO). To achieve coordinated ex- ...
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STIM1 at the plasma membrane as a new target in progressive chronic lymphocytic leukemia
... was related to mutated IgHV patients, a reduced level of cell surface (s) IgM, and a defective signalosome. In contrast, CLL cases with a worse clinical outcome show an elevated basal Ca 2+ level that can be ...
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4038.full.pdf
... For statistical analysis, we divided metaphases with varying degrees of chromosomal damage into four bins: 1-10 chromosome breaks/cell; >10 chromosome breaks/cell and <50% chromosomes fragmented ...
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Selection and screening strategies in directed evolution to improve protein stability
... display (Pan et al. 2012) and gas vesicle nanoparticles dis- play (DasSarma and DasSarma 2015). Spores of Bacillus subtilis are coated by two protective layers consisting of more than 70 kinds of distinct proteins (Pan ...
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